Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-500AFP488 | |||
| Alexa Fluor Plus 555 | NB600-500AFP555 | |||
| Alexa Fluor Plus 594 | NB600-500AFP594 | |||
| Alexa Fluor Plus 647 | NB600-500AFP647 | |||
| Alexa Fluor Plus 680 | NB600-500AFP680 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB120-6652 | |||
| FITC | NBP1-97372 | |||
| HRP | NB120-6651 | |||
| PE | NBP1-97335 | |||
| Rhodamine | NBP1-97383-0.5mg | |||
| Texas Red | NB110-3647 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-938AFP488 | |||
| Alexa Fluor Plus 555 | NB600-938AFP555 | |||
| Alexa Fluor Plus 594 | NB600-938AFP594 | |||
| Alexa Fluor Plus 647 | NB600-938AFP647 | |||
| Alexa Fluor Plus 680 | NB600-938AFP680 | |||
| Biotin | NB600-939-0.1mg | |||
| HRP | NB120-7339-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26573-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26257-50ul | |||
![Western Blot: Histone H2Av [p Ser137] Antibody [NBP1-78103] - Shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection. Personal Communication. YikA/G Rong, NIH, CCR, Bethesda, MD.](http://images.novusbio.com/fullsize/Histone-H2Av-[p-Ser137]-Antibody-Western-Blot-NBP1-78103-img0002.jpg "Western Blot: Histone H2Av [p Ser137] Antibody [NBP1-78103] - Shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection. Personal Communication. YikA/G Rong, NIH, CCR, Bethesda, MD.")
![Immunocytochemistry/Immunofluorescence: Histone H2Av [p Ser137] Antibody [NBP1-78103]](http://images.novusbio.com/fullsize/Histone-H2Av-[p-Ser137]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78103-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H2Av [p Ser137] Antibody [NBP1-78103] - Human Bladder Cancer.](http://images.novusbio.com/fullsize/Histone-H2Av-[p-Ser137]-Antibody-Immunohistochemistry-Paraffin-NBP1-78103-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2Av [p Ser137] Antibody [NBP1-78103]](http://images.novusbio.com/fullsize/Histone-H2Av-[p-Ser137]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78103-img0004.jpg)
![Immunohistochemistry: SDPR Antibody [NBP1-44090] - Tissue: Spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated with 5 ug/mL for 1 h at RT.](http://images.novusbio.com/fullsize/SDPR-Antibody-Immunohistochemistry-NBP1-44090-img0001.jpg "Immunohistochemistry: SDPR Antibody [NBP1-44090] - Tissue: Spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated with 5 ug/mL for 1 h at RT.")
![Western Blot: Alkaline Phosphatase, Bacterial Antibody [Biotin] [NB600-500] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Bacterial-Antibody-[Biotin]-Western-Blot-NB600-500-img0003.jpg "Western Blot: Alkaline Phosphatase, Bacterial Antibody [Biotin] [NB600-500] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).")
![N/A BMP-10 [Biotin]](http://images.novusbio.com/fullsize2/DATA_BMP10_DY292605_ELISA_2417.jpg "N/A BMP-10 [Biotin]")
![Western Blot: HSP90 Antibody (4B5) [NBP3-15145] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissueAll lanes: HSP90 antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 85 KDa.](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Western-Blot-NBP3-15145-img0005.jpg "Western Blot: HSP90 Antibody (4B5) [NBP3-15145] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissueAll lanes: HSP90 antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 85 KDa.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4B5) [NBP3-15145] - Immunofluorescence staining of NIH/3T3 cells with at HSP90 antibody 1:26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunocytochemistry-Immunofluorescence-NBP3-15145-img0001.jpg)
![Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145] - IHC image of HSP90 antibody diluted at 1:80 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunohistochemistry-NBP3-15145-img0004.jpg)
![Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145] - IHC image of HSP90 antibody diluted at 1:80 and staining in paraffin-embedded human endometrial cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunohistochemistry-NBP3-15145-img0003.jpg)
![Immunoprecipitation: HSP90 Antibody (4B5) [NBP3-15145] - Immunoprecipitating HSP90 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of HSP90 antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: HSP90 antibody (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunoprecipitation-NBP3-15145-img0002.jpg)
![Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Laminin at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-Immunohistochemistry-NB120-6571-img0002.jpg "Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Laminin at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.")
![Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: Placenta. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-Immunohistochemistry-NB120-6571-img0001.jpg)
![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0005.jpg "Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.")
![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Fluorescein conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0004.jpg)
![ELISA: Biotin Antibody [NB120-6643] - Analysis using BIOTIN Antibody DyLight 488.](http://images.novusbio.com/fullsize/Biotin-Antibody-ELISA-NB120-6643-img0001.jpg)
![ELISA: Biotin Antibody [NB120-6643] - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 ug of Bovine Albumin Biotin Conjugated. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Biotin Antibody-ELISA-NB120-6643-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha 2-Macroglobulin Antibody-Western Blot-NB600-938-img0010.jpg "Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.")
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis using the Biotin conjugate of NB600-938. Detection of Lane 1: . Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0006.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0009.jpg)
![Western Blot: PIP5K2B Antibody [NBP1-44261] - Lane 1: Mouse Brain homogenate samples. Load: 10 ug per lane. Primary antibody: PIP5K2B antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.](http://images.novusbio.com/fullsize/PIP5K2B-Antibody-Western-Blot-NBP1-44261-img0003.jpg "Western Blot: PIP5K2B Antibody [NBP1-44261] - Lane 1: Mouse Brain homogenate samples. Load: 10 ug per lane. Primary antibody: PIP5K2B antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.")
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845324.jpg "Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845328.jpg)
![Western Blot: c-Fos Antibody (14C10) [NBP3-26573] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: c-Fos Antibody at 0.81ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41, 29, 37 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202411351611.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (14C10) [NBP3-26573] - Staining of HepG2 cells with c-Fos Antibody (14C10) at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-28220241249368.jpg)
![Flow Cytometry: c-Fos Antibody (14C10) [NBP3-26573] - Overlay histogram showing Hela cells stained with c-Fos Antibody (14C10) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202414183668.jpg)
![Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: liver. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY2-Antibody-Immunohistochemistry-NBP2-13158-img0003.jpg "Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: liver. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.")
![Immunohistochemistry-Paraffin: SPRY2 Antibody [NBP2-13158] - Immunohistochemistry-Paraffin: Sprouty 2 - Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.](http://images.novusbio.com/fullsize/SPRY2-Antibody-Immunohistochemistry-Paraffin-NBP2-13158-img0001.jpg)
![Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: kidney. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY2-Antibody-Immunohistochemistry-NBP2-13158-img0002.jpg)
![Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug). Lane 3: U251 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-26220241322156.jpg "Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug). Lane 3: U251 whole cell lysate (10ug).")
![Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-26220241535573.jpg)
![Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-27220248471918.jpg)
![Western Blot: p97/VCP Antibody (5H12) [NBP3-26257] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, Rat brain tissue, Rat liver tissue. All lanes: p97/VCP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 kDa Observed band size: 90 kDa](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-28220241144267.jpg)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5H12) [NBP3-26257] - Staining of SY5Y Cells with p97/VCP Antibody (5H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-282202412493642.jpg)