Alkaline Phosphatase, Bacterial Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Ec
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

Alkaline Phosphatase, Bacterial Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: Alkaline Phosphatase, Bacterial, Bacterial [E.coli] (Uniprot: P00634)
• Specificity: Cross reactivity against Alkaline Phosphatase, Bacterial from other tissues and species may occur but have not been specifically determined. Some minimal cross reactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: phoA
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Cross reactivity against Alkaline Phosphatase, Bacterial from other tissues and species may occur but have not been specifically determined. Some minimal cross reactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Multi-step

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alkaline Phosphatase, Bacterial Antibody [Alexa Fluor™ Plus 488]

• BAP
• ECK0378
• JW0374
• Pho A
• PhoA

Background

Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: phoA