Histone H2Av [p Ser137] Antibody - BSA Free

Western Blot: Histone H2Av [p Ser137] Antibody [NBP1-78103] - Shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection. Personal Communication. YikA/G Rong, NIH, CCR, Bethesda, MD.

Immunocytochemistry/Immunofluorescence: Histone H2Av [p Ser137] Antibody [NBP1-78103]

Immunohistochemistry-Paraffin: Histone H2Av [p Ser137] Antibody [NBP1-78103] - Human Bladder Cancer.

Immunocytochemistry/Immunofluorescence: Histone H2Av [p Ser137] Antibody [NBP1-78103]

• Reactivity: Dr
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2Av [p Ser137] Antibody - BSA Free Summary

• Description: Affinity purified Anti-Histone H2AvD [p Ser137] Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the [p Ser137] residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Histone H2Av [p Ser137] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein. (Uniprot: P08985)
• Modification: p Ser137
• Specificity: Affinity purified Anti-Histone Histone H2Av pS137 Antibody is directed against the phosphorylated form of Drosophila Histone H2Av protein at the pS137 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila Histone H2Av pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila Histone H2Av is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with Histone H2Av proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: His2Av
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:29000-1:30000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1:400-1:1600
• Application Notes: This product is tested in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.
  
  Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27198229).
  
  Use in IHC-Fr reported in scientific literature (PMID:33485066)

Reactivity Notes

A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Histone H2Av [p Ser137] Antibody - BSA Free

• CG5499 gene product from transcript CG5499-RA
• gamma-His2Av
• H2AX
• 5499
• CG5499
• CG5499-PA
• gamma-H2Av
• gamma-HIS2AV
• H2A.F/Z
• H2a.V
• H2A.Z
• H2av
• H2AV_DROM
• h2AvD
• H2AZ
• his
• His2
• HIS2AV
• His2AvD
• HisH2Av
• Hist
• Hist2av
• Histone H2A variant
• l(3)05146
• l(3)810
• l(3)97Dd
• l(3)L1602

Background

Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H2Av Antibody (NBP1-78103)(1)

Publication using NBP1-78103

• Huang F, Saraf A, Florens L et al. The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition. Genes Dev. 2016-05-15 [PMID: 27198229] (ICC/IF, Drosophila)

FAQs for Histone H2Av Antibody (NBP1-78103). (Showing 1 - 1 of 1 FAQ).

1. I am interested in antibody against Drosophila H2Av protein (NBP1-78103). According to the description this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) Histone H2Av protein. But according to Fly base there are only 101 aa in this protein. I am confused. Can you clarify this for me?
   • The immunogen is based on the protein P08985 found on UniProt (https://www.uniprot.org/uniprotkb/P08985/entry). It contains 141 amino acids.

Bioinformatics

• Gene Symbol: His2Av
• Uniprot:
  • P08985(Drosophila)