Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97469SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68136SS | |||
| Azide and BSA Free | NBP2-80973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1071SS | |||
| Azide and BSA Free | NBP2-80642 | |||
![Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Western-Blot-NBP1-97469-img0004.jpg "Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).")
![Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at 1:500 in PBST, 0.1% triton + 10% DS 1h RT. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunocytochemistry-Immunofluorescence-NBP1-97469-img0006.jpg)
![Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunohistochemistry-Paraffin-NBP1-97469-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunocytochemistry-Immunofluorescence-NBP1-97469-img0005.jpg)
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Intracellular-NB110-68136-img0010.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].")
![<b>Biological Strategies Validation. </b>Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-BSA-Free-Flow-Cytometry-NB110-68136-img0016.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0008.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0007.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0011.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0013.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0014.jpg)
![N/A Cystatin C [HRP]](http://images.novusbio.com/fullsize2/DATA_Cystatin_C_MSCTC0_ELISA_1030.jpg "N/A Cystatin C [HRP]")
![N/A Cystatin C [HRP]](http://images.novusbio.com/fullsize2/Cystatin_C_MSCTC0_ELISA_322.jpg)
![N/A Cystatin C [HRP]](http://images.novusbio.com/fullsize2/Cystatin_C_MSCTC0_ELISA_321.jpg)
![N/A IL-22 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL22_M2200_ELISA_927.jpg "N/A IL-22 [HRP]")
![N/A IL-22 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL22_M2200_ELISA_928.jpg)
![N/A IL-22 [HRP]](http://images.novusbio.com/fullsize2/IL-22_M2200_ELISA_231.jpg)
![N/A IL-22 [HRP]](http://images.novusbio.com/fullsize2/IL-22_M2200_ELISA_230.jpg)
![Western Blot: SPINK1 Antibody (4D4) [H00006690-M01] - Detection against Immunogen (31.9 kDa). Antibody reactive against recombinant protein.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Western-Blot-H00006690-M01-img0010.jpg "Western Blot: SPINK1 Antibody (4D4) [H00006690-M01] - Detection against Immunogen (31.9 kDa). Antibody reactive against recombinant protein.")
![Immunohistochemistry-Paraffin: SPINK1 Antibody (4D4) [H00006690-M01] - Analysis of SPINK1 antibody (4D4) on mouse pancreas tissue. Antibody concentration 1 ug/ml. Image from verified customer review.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunohistochemistry-Paraffin-H00006690-M01-img0016.jpg)
![Western Blot: SPINK1 Antibody (4D4) [H00006690-M01] - SPINK1 monoclonal antibody (M01), clone 4D4. Analysis of SPINK1 expression in human pancreas.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Western-Blot-H00006690-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: SPINK1 Antibody (4D4) [H00006690-M01] - Analysis of monoclonal antibody to SPINK1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunohistochemistry-Paraffin-H00006690-M01-img0006.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - Representative images for immunohistochemical staining of SPINK1in 22RV1 xenograft tumors excised from orchiectomized mice treated with enzalutamide (20mg/kg body weight) or vehicle control (n=5 each). Intact group represents non-castrated control mice (n=5). Scale bar represents 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14184-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunohistochemistry-H00006690-M01-img0012.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - Representative images showing H&E staining (x200 magnification) and immunostaining (x200 magnification) for AR, synaptophysin, and SPINK1 in tumor specimens obtained from NEPC patients', namely WCM12, WCM155 (an organoid), and WCM677. Scale bar represents 100um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14184-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunohistochemistry-H00006690-M01-img0013.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - IHC staining for SPINK1 and NPY of an adjacent section on the ST array. Nuclei are stained with DAPI (blue). Scale bar indicates 1mm. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04724-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunohistochemistry-H00006690-M01-img0014.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - ADT induced SPINK1 upregulation associates with NE-phenotype in mice and NEPC patients. Representative images of immunohistochemical staining for the same markers shown in a using VCaP xenograft tumors. Scale bar represents 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-14184-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunohistochemistry-H00006690-M01-img0015.jpg)
![Immunoprecipitation: SPINK1 Antibody (4D4) [H00006690-M01] - Analysis of SPINK1 transfected lysate using anti-SPINK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SPINK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-Immunoprecipitation-H00006690-M01-img0007.jpg)
![ELISA: SPINK1 Antibody (4D4) [H00006690-M01] - Detection limit for recombinant GST tagged SPINK1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SPINK1-Antibody-4D4-ELISA-H00006690-M01-img0011.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - ADT induced SPINK1 upregulation associates with NE-phenotype in mice & NEPC patients.a Box plots depicting relative expression of SPINK1, SYP, CHGA, TUBB3, & VIM transcripts (read counts) in VCaP tumors implanted orthotopically in orchiectomized mice & subjected to vehicle (n = 4) or anti-androgens [enzalutamide (n = 4) or ARN-509 (n = 4)] treatment for 4 weeks (GSE95413). b Representative images of immunohistochemical staining for the same markers shown in a using VCaP xenograft tumors as described in a. Scale bar represents 100 μm. c Box plots depicting quantification of the immunohistochemical staining in VCaP xenografts for the markers shown in b. d Representative images for immunohistochemical staining of SPINK1, SYP, CHGA, & TUBB3 in 22RV1 xenograft tumors excised from orchiectomized mice treated with enzalutamide (20 mg/kg body weight) or vehicle control (n = 5 each). Intact group represents non-castrated control mice (n = 5). Scale bar represents 50 μm. e Box plots depicting quantification of the immunohistochemical staining in 22RV1 xenografts for the markers shown in d. f Representative images showing H&E staining (×200 magnification) & immunostaining (×200 magnification) for AR, synaptophysin, & SPINK1 in tumor specimens obtained from NEPC patients’, namely WCM12, WCM155 (an organoid), & WCM677. Scale bar represents 100 µm. Data are presented as box-and-whisker plots with median, where the box extends from 25th–75th percentile, & whiskers ranges from minimum & maximum values. For panels a, c, e one-way ANOVA, Dunnett’s multiple-comparisons test was applied. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006690-m01_mouse-monoclonal-spink1-antibody-4d4-31020241548221.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - SPINK1 is negatively correlated with AR in PCa patients.a Heatmap depicting AR & SPINK1 expression in TCGA-PRAD cohort (n = 180). Shades of yellow & blue represents expression values in log2 (RPM+1). b Representative micrographs depicting PCa tissue microarray (TMA) cores (n = 237), immunostained for SPINK1 & AR expression by immunohistochemistry (IHC). Top panel shows representative IHC for SPINK1 in SPINK1-negative (SPINK1−) & SPINK1-positive (SPINK1+) patients. Bottom panel represents IHC for AR expression in the tumor core from same patients. Scale bar represents 500 µm & 100 µm for the entire core & the inset images, respectively. c Bar plot showing percentage of IHC scoring for AR in the SPINK1+ & SPINK1− patients’ specimens. P-value for the Chi-Square test is indicated. d Contingency table for the AR & SPINK1 status. Patients showing high or medium expression of AR were grouped as AR-(Hi/Med), while patients with low or null AR expression were indicated as AR-(Low/Neg). P-value for Fisher’s exact test is indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006690-m01_mouse-monoclonal-spink1-antibody-4d4-310202415485141.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - Spatial gene expression heterogeneity within the 1.2 cancer tissue sample. a Factor activity maps for selected factors corresponding to epithelial, stromal, cancerous, PIN, or inflamed regions. Remaining factors’ activity maps in Supplementary Figure 2 & Supplementary Data 1. b Annotated brightfield image of H&E-stained tissue section. c Heatmap of the 20 most variable genes between cancer, PIN & normal gland regions, using spot sets from Supplementary Fig. 4b. Centered rlog: difference of rlog (variance-stabilized transform of ST expression data) & gene-wise mean rlog. Arrows highlight genes of interest validated by immunohistochemistry (IHC). d First two principal components of spot sets from c separate cancer, PIN & normal regions. e Array dot plots for SPINK1 & NPY. Circle size in array dot plots indicates normalized ST counts. f IHC staining for SPINK1 & NPY of an adjacent section on the ST array. Nuclei are stained with DAPI (blue). Scale bar indicates 1 mm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29925878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006690-m01_mouse-monoclonal-spink1-antibody-4d4-310202415483612.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - ADT induced SPINK1 upregulation associates with NE-phenotype in mice & NEPC patients.a Box plots depicting relative expression of SPINK1, SYP, CHGA, TUBB3, & VIM transcripts (read counts) in VCaP tumors implanted orthotopically in orchiectomized mice & subjected to vehicle (n = 4) or anti-androgens [enzalutamide (n = 4) or ARN-509 (n = 4)] treatment for 4 weeks (GSE95413). b Representative images of immunohistochemical staining for the same markers shown in a using VCaP xenograft tumors as described in a. Scale bar represents 100 μm. c Box plots depicting quantification of the immunohistochemical staining in VCaP xenografts for the markers shown in b. d Representative images for immunohistochemical staining of SPINK1, SYP, CHGA, & TUBB3 in 22RV1 xenograft tumors excised from orchiectomized mice treated with enzalutamide (20 mg/kg body weight) or vehicle control (n = 5 each). Intact group represents non-castrated control mice (n = 5). Scale bar represents 50 μm. e Box plots depicting quantification of the immunohistochemical staining in 22RV1 xenografts for the markers shown in d. f Representative images showing H&E staining (×200 magnification) & immunostaining (×200 magnification) for AR, synaptophysin, & SPINK1 in tumor specimens obtained from NEPC patients’, namely WCM12, WCM155 (an organoid), & WCM677. Scale bar represents 100 µm. Data are presented as box-and-whisker plots with median, where the box extends from 25th–75th percentile, & whiskers ranges from minimum & maximum values. For panels a, c, e one-way ANOVA, Dunnett’s multiple-comparisons test was applied. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006690-m01_mouse-monoclonal-spink1-antibody-4d4-31020241548366.jpg)
![Immunohistochemistry: SPINK1 Antibody (4D4) [H00006690-M01] - ADT induced SPINK1 upregulation associates with NE-phenotype in mice & NEPC patients.a Box plots depicting relative expression of SPINK1, SYP, CHGA, TUBB3, & VIM transcripts (read counts) in VCaP tumors implanted orthotopically in orchiectomized mice & subjected to vehicle (n = 4) or anti-androgens [enzalutamide (n = 4) or ARN-509 (n = 4)] treatment for 4 weeks (GSE95413). b Representative images of immunohistochemical staining for the same markers shown in a using VCaP xenograft tumors as described in a. Scale bar represents 100 μm. c Box plots depicting quantification of the immunohistochemical staining in VCaP xenografts for the markers shown in b. d Representative images for immunohistochemical staining of SPINK1, SYP, CHGA, & TUBB3 in 22RV1 xenograft tumors excised from orchiectomized mice treated with enzalutamide (20 mg/kg body weight) or vehicle control (n = 5 each). Intact group represents non-castrated control mice (n = 5). Scale bar represents 50 μm. e Box plots depicting quantification of the immunohistochemical staining in 22RV1 xenografts for the markers shown in d. f Representative images showing H&E staining (×200 magnification) & immunostaining (×200 magnification) for AR, synaptophysin, & SPINK1 in tumor specimens obtained from NEPC patients’, namely WCM12, WCM155 (an organoid), & WCM677. Scale bar represents 100 µm. Data are presented as box-and-whisker plots with median, where the box extends from 25th–75th percentile, & whiskers ranges from minimum & maximum values. For panels a, c, e one-way ANOVA, Dunnett’s multiple-comparisons test was applied. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006690-m01_mouse-monoclonal-spink1-antibody-4d4-310202415483636.jpg)
![N/A CCL17/TARC [HRP]](http://images.novusbio.com/fullsize2/DATA_CCL17_DDN00_ELISA_651.jpg "N/A CCL17/TARC [HRP]")
![N/A CCL17/TARC [HRP]](http://images.novusbio.com/fullsize2/DATA_CCL17_DDN00_ELISA_652.jpg)
![N/A CCL17/TARC [HRP]](http://images.novusbio.com/fullsize2/CCL17_DDN00_ELISA_78.jpg)
![N/A Galectin-3 [HRP]](http://images.novusbio.com/fullsize2/DATA_Galectin3_DGAL30_ELISA_688.jpg "N/A Galectin-3 [HRP]")
![N/A Galectin-3 [HRP]](http://images.novusbio.com/fullsize2/DATA_Galectin3_DGAL30_ELISA_689.jpg)
![N/A Galectin-3 [HRP]](http://images.novusbio.com/fullsize2/Galectin-3_DGAL30_ELISA_101.jpg)
![N/A MMP-9 [HRP]](http://images.novusbio.com/fullsize2/DATA_MMP9_MMPT90_ELISA_1006.jpg "N/A MMP-9 [HRP]")
![N/A MMP-9 [HRP]](http://images.novusbio.com/fullsize2/MMP-9_MMPT90_ELISA_296.jpg)
![N/A Chitinase 3-like 1/YKL-40 [HRP]](http://images.novusbio.com/fullsize2/DATA_Chitinase_3like_1_DC3L10_ELISA_601.jpg "N/A Chitinase 3-like 1/YKL-40 [HRP]")
![N/A Chitinase 3-like 1/YKL-40 [HRP]](http://images.novusbio.com/fullsize2/Chitinase_3-like_1_DC3L10_ELISA_45.jpg)
![N/A Endoglin/CD105 [HRP]](http://images.novusbio.com/fullsize2/DATA_Endoglin_DNDG00_ELISA_774.jpg "N/A Endoglin/CD105 [HRP]")
![N/A Endoglin/CD105 [HRP]](http://images.novusbio.com/fullsize2/Endoglin_DNDG00_ELISA_455.jpg)
![N/A beta-NGF [Biotin]](http://images.novusbio.com/fullsize2/DATA_betaNGF_DY256_ELISA_1847.jpg "N/A beta-NGF [Biotin]")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Flow-Cytometry-NB600-1071-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor vasculature which are originating from the host (mouse).](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which is an expected outcome for this clone. This section was included in NB600-1071's IHC validation testing as a negative control.](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin. Notably, the antibody specifically stained the endothelial cells of the tumor vasculature which is originating from the host animal cells (mouse). This observation was verified by including a negative control in this validation testing (see the IHC image of human renal cancer tissue section with negative signal for CD34).](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The staining was detected using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin. Note that this murine CD34 specific antibody stained only the endothelial cells of the tumor vasculature which is originating from the host animal cells (mouse). This observation was verified by including a negative control in this validation testing (see the IHC image of human renal cancer tissue section with negative signal for CD34).](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the colon.](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0012.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the heart tissue.](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Immunohistochemistry-Paraffin-NB600-1071-img0013.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/fullsize/CD34-Antibody-MEC-14-7-Flow-Cytometry-NB600-1071-img0015.jpg)