Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-602AFP488 | |||
| Alexa Fluor Plus 555 | NB600-602AFP555 | |||
| Alexa Fluor Plus 594 | NB600-602AFP594 | |||
| Alexa Fluor Plus 647 | NB600-602AFP647 | |||
| Alexa Fluor Plus 680 | NB600-602AFP680 | |||
| HRP | NB120-7635-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | PDB7H30 | |||
| Carrier Free | SB7H30 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54574-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB9626P-100UG | |||
| Carrier Free | MAB9626-SP | |||
| Carrier Free | FAB9626V-100UG | |||
| Carrier Free | FAB9626U-100UG | |||
| Carrier Free | FAB9626T-100UG | |||
| Carrier Free | FAB9626S-100UG | |||
| Carrier Free | FAB9626R-100UG | |||
| Carrier Free | FAB9626N-100UG | |||
| Carrier Free | FAB9626G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51541SS | |||
![N/A IL-17C [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17C_DY1234_ELISA_1607.jpg "N/A IL-17C [Biotin]")
![N/A Cathepsin L [Biotin]](http://images.novusbio.com/fullsize2/DATA_Cathepsin_L_DY952_ELISA_2273.jpg "N/A Cathepsin L [Biotin]")
![N/A IL-36 beta/IL-1F8 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL36_beta_DY109905_ELISA_2340.jpg "N/A IL-36 beta/IL-1F8 [Biotin]")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0014.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0010.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0011.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0012.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0013.jpg)
![N/A B7-H3/CD276 [HRP]](http://images.novusbio.com/fullsize2/DATA_B7H3_DB7H30_ELISA_583.jpg "N/A B7-H3/CD276 [HRP]")
![N/A B7-H3/CD276 [HRP]](http://images.novusbio.com/fullsize2/B7-H3_DB7H30_ELISA_34.jpg)
![Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Western-Blot-NBP2-53295-img0006.jpg "Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1386).")
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with Ferritin Light Chain Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Immunohistochemistry-Paraffin-NBP2-53295-img0009.jpg)
![Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Western Blot of A431, HeLa, Liver and Testis lysate using Ferritin Light Chain Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Western-Blot-NBP2-53295-img0005.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Immunohistochemistry-Paraffin-NBP2-53295-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Immunohistochemistry-Paraffin-NBP2-53295-img0002.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded human kidney stained with Biotinylated Ferritin Light Chain Mouse Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Immunohistochemistry-Paraffin-NBP2-53295-img0007.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with Ferritin Light Chain Mouse Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Immunohistochemistry-Paraffin-NBP2-53295-img0008.jpg)
![Protein Array: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ferritin Light Chain Antibody (FTL/1386) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Protein-Array-NBP2-53295-img0004.jpg)
![N/A Atrial Natriuretic Peptide/ANP [HRP]](http://images.novusbio.com/fullsize2/Atrial_Natriuretic_Peptide_DANP00_ELISA_1155.jpg "N/A Atrial Natriuretic Peptide/ANP [HRP]")
![N/A Atrial Natriuretic Peptide/ANP [HRP]](http://images.novusbio.com/fullsize2/Atrial_Natriuretic_Peptide_DANP00_ELISA_1154.jpg)
![N/A beta 2-Microglobulin [Biotin]](http://images.novusbio.com/fullsize2/beta_2Microglobulin_KGE019_ELISA_1153.jpg "N/A beta 2-Microglobulin [Biotin]")
![N/A beta 2-Microglobulin [Biotin]](http://images.novusbio.com/fullsize2/beta_2Microglobulin_KGE019_ELISA_1152.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize2/DATA_CXCL8_IL8_CA8000_ELISA_521.jpg "N/A CXCL8/IL-8 [HRP]")
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize2/CXCL8_CA8000_ELISA_5.jpg)
![Western Blot: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis against truncated Prostate Specific Antigen recombinant protein.](http://images.novusbio.com/fullsize/Kallikrein-3-PSA-Antibody-5A11E9-Western-Blot-NBP1-51541-img0012.jpg "Western Blot: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis against truncated Prostate Specific Antigen recombinant protein.")
![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using Prostate Specific Antigen mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Kallikrein-3-PSA-Antibody-5A11E9-Immunohistochemistry-Paraffin-NBP1-51541-img0011.jpg)
![Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Flow cytometric analysis of PC-3 cells using Prostate Specific Antigen mouse mAb (right) and negative control (left).](http://images.novusbio.com/fullsize/Kallikrein-3-PSA-Antibody-5A11E9-Flow-Cytometry-NBP1-51541-img0009.jpg)
![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate tissues using Prostate Specific Antigen mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Kallikrein-3-PSA-Antibody-5A11E9-Immunohistochemistry-Paraffin-NBP1-51541-img0010.jpg)