Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-506SS | |||
| Azide and BSA Free | NBP2-80571 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25158-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-56726 | |||
| BSA Free | NB100-56722SS | |||
| Azide and BSA Free | NB100-56726SS | |||
![N/A PLGF [HRP]](http://images.novusbio.com/fullsize2/DATA_PlGF_DPG00_ELISA_788.jpg "N/A PLGF [HRP]")
![N/A PLGF [HRP]](http://images.novusbio.com/fullsize2/DATA_PlGF_DPG00_ELISA_789.jpg)
![N/A PLGF [HRP]](http://images.novusbio.com/fullsize2/PlGF_DPG00_ELISA_158.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0013.jpg "Western Blot: V5 Epitope Tag Antibody [NB600-381] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - ATG2A-V5 overexpression reduces intracellular neutral lipids in THP-1 cells. Scale bars, 10 um. Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection. Nat Commun (2017)](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-381-img0009.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0002.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 ug/mL (1:5000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0001.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - Analysis using the HRP conjugate of NB600-381. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/mL (1:5,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - HEK293 cells transiently expressing V5-tagged mutant PFN1 stained with V5 Epitope Tag antibody (red). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-381-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green. pAb dilution of 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunohistochemistry-NB600-381-img0008.jpg)
![Immunoprecipitation: V5 Epitope Tag Antibody [NB600-381] - HEK293T were transfected with V5-KU86 plasmid, followed by V5 antibody IP then Western blot with v5 antibody (NB600-381). Primary antibody at 1:1000, 4C, overnight. IP/WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunoprecipitation-NB600-381-img0012.jpg)
![Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] - (a) Light & fluorescent micrographs of stable HN33.11 cells expressing (A)-GFP or (B)-GFP, respectively. Results from two stable clones of each transfection pool are shown. Cells are treated with vehicle (Dox−) or 1 μg/mL Dox (Dox+). (b) Western blot of homogenates of stable clones showing endogenous production of full-length p75NTR (FLp75), Dox-induced, but “leaky” production of (A) & (B) & of the V5 tag. Control values for each clone do not differ significantly between GFP(−) & GFP(+) cells (Student's t-test). ***P < 0.001 relative to GFP(−) cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21904642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-381_rabbit-polyclonal-v5-epitope-tag-antibody-31020241539253.jpg)
![Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] - Mapping the PRDX2 & PRDX4 binding domains of HIF-1 alpha A. & B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector & WCL was incubated with purified GST or GST-HIF-1 alpha fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-381_rabbit-polyclonal-v5-epitope-tag-antibody-310202415304231.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0021.jpg "Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - NIH3T3 cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-NB600-506-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - Rat FR cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0023.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0015.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunohistochemistry-Paraffin-NB600-506-img0005.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0017.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0019.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0002.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Western-Blot-NB600-506-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Immunocytochemistry-Immunofluorescence-NB600-506-img0013.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0012.jpg)
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Flow-Cytometry-NB600-506-img0016.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Tissue levels of VEGF & PCNA.VEGF levels were estimated by (A) western blot & (B) ELISA. (C) PCNA levels estimated by western blot. Data were normalized with tubulin. (D) Representative images of PCNA immunohistochemistry staining in the nucleus is shown for CS-exposed mice (CSE) & CS-exposed & LGF-treated mice (CSE+LGF). Scale bars = 50 µm. LGF promoted an increase of PCNA+ cells as showed in the bar graph. ** P<0.01 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-310202415304283.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - LGF ameliorates oxidative stress.Bars represent (A) 3NT & (B) Nrf2 levels estimated by western blot. Data were normalized with tubulin. * P<0.05 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241539740.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Tissue levels of VEGF & PCNA.VEGF levels were estimated by (A) western blot & (B) ELISA. (C) PCNA levels estimated by western blot. Data were normalized with tubulin. (D) Representative images of PCNA immunohistochemistry staining in the nucleus is shown for CS-exposed mice (CSE) & CS-exposed & LGF-treated mice (CSE+LGF). Scale bars = 50 µm. LGF promoted an increase of PCNA+ cells as showed in the bar graph. ** P<0.01 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241534356.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - LGF ameliorates oxidative stress.Bars represent (A) 3NT & (B) Nrf2 levels estimated by western blot. Data were normalized with tubulin. * P<0.05 vs. air-exposed mice; ¥ P<0.05 vs. CS-exposed mice (n = 5 per group). Data are presented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25401951), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-310202415284516.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Role of cell-type-specific cyclooxygenase-2 (Cox-2) expression in Ras/p53-mediated tumor formation. a Experimental scheme. b Microscopic phenotype & histology of three-dimensional (3D) organoids with/without celecoxib treatment. c Relative 3D organoid formation was determined by the number of organoids larger than 100 µm, n = 3 independent experiments, 25 fields at high power field (HPF) per sample. Veh, vehicle control; C25, 25 µM; C50, 50 µM celecoxib. d Experimental scheme. e Ras/p53-mediated tumor incidence with/without condition Cox-2 knockout. Ptgs2wt/wt, n = 15 animals; Ptgs2flox/flox, n = 14 animals. f, g Histological phenotypes. h–k Immunoblotting of Keratin5 (Krt5), Krt6 & Loricrin (Lor) demonstrated decreased tumor susceptibility but increased differentiation status, n = 4 independent experiments. l Summary of the contribution of physiological stress factors in the susceptibility of tumor development from tumor-competent Krt15+ progenitors. Data with bar graphs are represented as mean ± SEM. Statistical significance was determined by pair-wise comparison using t test; ns = not significant, *p < 0.05, **p < 0.005. Scale bars, 100 µm. Ptgs2 prostaglandin-endoperoxide synthase 2 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31110179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241616019.jpg)
![Western Blot: alpha Tubulin Antibody (YL1/2) - BSA Free [NB600-506] - Microenvironmental acidic stressors & tumor susceptibility. a Experimental scheme. Epithelial cells isolated from the regions adjacent or distant from squamocolumnar junction (SCJ). b Microscopic phenotype of three-dimensional (3D) organoids from control & Krt15-CrePR; LSL-KrasG12D; p53flox/flox mice. Histology of 3D organoids demonstrated that oncogenic Ras/p53 increased abnormal growth features. c–e Quantification of relative organoid formation (n = 3 independent experiments, 25 fields at high power field (HPF) per sample) & size distribution (n ≥ 150) from control & experimental mice expressing oncogenic Ras/p53 combination. f Experimental scheme. Phosphate-buffered saline (PBS) (vehicle) or PPI were daily treated by intraperitoneal (i.p.) injections (5 consecutive days per week). g Tumor incidence from Krt15-CrePR; LSL-KrasG12D; p53flox/flox mice, with/without PPI treatment, n = 9 animals per each. Statistical significance was determined by Fisher’s exact test; *p < 0.05. h–j Immunoblotting of Keratin5 (Krt5) & Krt6 demonstrated relatively less tumor formation in the PPI treatment group, n = 5 independent experiments. k–m Immunostaining of Krt5, Krt6, & phospho-histone H3 (ph-H3), & histology demonstrated suppressed tumor formation by daily PPI treatment in Krt15-CrePR; LSL-KrasG12D; p53flox/flox mice. PPI, proton pump inhibitor. Data with bar graphs are represented as mean ± SEM. Statistical significance was determined by pair-wise comparison using t test; *p < 0.05, **p < 0.005. Scale bars, 100 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31110179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-506_rat-monoclonal-alpha-tubulin-antibody-yl1-2-31020241618944.jpg)
![N/A CCL3/MIP-1 alpha [HRP]](http://images.novusbio.com/fullsize2/DATA_CCL3_MMA00_ELISA_999.jpg "N/A CCL3/MIP-1 alpha [HRP]")
![N/A CCL3/MIP-1 alpha [HRP]](http://images.novusbio.com/fullsize2/CCL3_MMA00_ELISA_291.jpg)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25158-img0005.jpg "Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.")
![Western Blot: mCherry Antibody [NBP2-25158] - Analysis of NBP2-25158. Lane 1. Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector. There is a strong clean band at about 29kDa corresponding to mCherry (predicted molecular weight ~27kDa). Lane 2, lysate of untransfected HEK293 cells show no protein bands. The NBP2-25158 antibody was used at 1:2,000 dilution.](http://images.novusbio.com/fullsize/mCherry-Antibody-NBP2-25158-img0004.jpg)
![Western Blot: mCherry Antibody [NBP2-25158] - Analysis of HEK293 cell lysates, and recombinant protein solutions using chicken mCherry pAb, dilution 1:2000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with an mCherry-HA constract, [4] pure recombinant mCherry protein (predicted molecular weight ~ 27kDa), [5] pure recombinant GFP protein and [6] HEK293 transfected with a GFP construct. The major band at about 30kDa corresponds to mCherry-HA protein and the slightly larger recombinant form runs at about 33kDa due to presence of a His tag and other vector derived sequence. The mCherry antibody reacts strongly with both mCherry constructs and does not react with GFP protein. The blot was simultaneously probed with mouse HSP60 mAb, dilution 1:10000 (Red), which reveals a band at 60kDa only in the cell lysates.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-25158-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - Dox-inducible DN Lats2-IRES-mCherry transgenic embryos were imaged before Dox treatment (top panel) and the same embryo was imaged following 24 hr of Dox live (middle panel) and fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, immunofluorescence stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos are circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 um. n indicates number of transgenic embryos analyzed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28226240/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25158-img0007.jpg)
![Western blot analysis of HEK293 cell lysates using chicken pAb to mCherry, NBP2-25158, dilution 1:2,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein molecular weight standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. NBP2-25158 recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.](http://images.novusbio.com/fullsize/nbp2-25158_chicken-polyclonal-mcherry-antibody-western-blot-264202414221..jpg)
![N/A TNF RII/TNFRSF1B [HRP]](http://images.novusbio.com/fullsize2/DATA_TNF_RII_DRT200_ELISA_817.jpg "N/A TNF RII/TNFRSF1B [HRP]")
![N/A TNF RII/TNFRSF1B [HRP]](http://images.novusbio.com/fullsize2/DATA_TNF_RII_DRT200_ELISA_818.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](http://images.novusbio.com/fullsize2/TNF_RII_DRT200_ELISA_176.jpg)
![N/A EGF [HRP]](http://images.novusbio.com/fullsize2/DATA_EGF_DEG00_ELISA_653.jpg "N/A EGF [HRP]")
![N/A EGF [HRP]](http://images.novusbio.com/fullsize2/DATA_EGF_DEG00_ELISA_654.jpg)
![N/A EGF [HRP]](http://images.novusbio.com/fullsize2/EGF_DEG00_ELISA_79.jpg)
![N/A Osteopontin/OPN [HRP]](http://images.novusbio.com/fullsize2/DATA_Osteopontin_DOST00_ELISA_783.jpg "N/A Osteopontin/OPN [HRP]")
![N/A Osteopontin/OPN [HRP]](http://images.novusbio.com/fullsize2/Osteopontin_DOST00_ELISA_155.jpg)
![N/A VEGF [HRP]](http://images.novusbio.com/fullsize2/DATA_VEGF_RRV00_ELISA_1097.jpg "N/A VEGF [HRP]")
![N/A VEGF [HRP]](http://images.novusbio.com/fullsize2/DATA_VEGF_RRV00_ELISA_1098.jpg)
![N/A VEGF [HRP]](http://images.novusbio.com/fullsize2/VEGF_RRV00_ELISA_364.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize2/DATA_IL12_p70_D1200_ELISA_531.jpg "N/A IL-12 p70/IL-12A [HRP]")
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize2/DATA_IL12_p70_D1200_ELISA_532.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize2/IL-12_D1200_ELISA_11.jpg)
![N/A MMP-9 [Biotin]](http://images.novusbio.com/fullsize2/DATA_MMP9_DY911_ELISA_2261.jpg "N/A MMP-9 [Biotin]")
![Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Flow-Cytometry-NB100-56722-img0005.jpg "Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.")
![Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Western-Blot-NB100-56722-img0015.jpg)
![Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TLR2 (NB100-56722) in 5% BSA-TBST and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Western-Blot-NB100-56722-img0016.jpg)
![Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Monitoring of TLR2 expression in normal B cells and primary MCL cells cultured for 24h in the absence/presence of CD40L, IL-4, and TLR ligands. Filled curves refer to isotypic control, empty curves to the TLR2 antibody (NB100-56722) of untreated samples. Dotted histograms represent CD40L+IL4 activated cells, dashed curves indicate CD40L+IL4 activated cells further treated with TLR ligands. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0153823) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Flow-Cytometry-NB100-56722-img0013.jpg)
![Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green: isotype control. Red: anti-TLR2 antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Flow-Cell-Surface-NB100-56722-img0010.jpg)
![Block/Neutralize: TLR2 Antibody (TL2.1) [NB100-56722] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR2 antibody (NB100-56722) for 1 h. Afterwards, DCs were infected with H. pylori G27 (MOI 5) and the levels of the costimulatory molecule CD86 and the maturation marker CD83 were analyzed by FACS on CD11c+ cells 24 h post-infection. Data are presented as mean +/- SD of five independent experiments. *p<=0.05, **p<=0.005, ***p<=0.0005. Asterisks on top of bars indicate significance relative to non-neutralized, H. pylori-primed control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104804) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Block-Neutralize-NB100-56722-img0014.jpg)
![Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722] - Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control; purple represents anti-TLR2 antibody.](http://images.novusbio.com/fullsize/nb100-56722_mouse-monoclonal-tlr2-antibody-tl2-1-17720241003212.jpg)