V5 Epitope Tag Antibody [Biotin]

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Western Blot: V5 Epitope Tag Antibody [Biotin] [NB600-381B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP ...read more

Product Details

Summary
Reactivity Ec, NASpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

V5 Epitope Tag Antibody [Biotin] Summary

Immunogen
Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.
Epitope
GKPIPNPLLGLDST
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:100000
  • ELISA 1:100-1:2000
Readout System
Publications
Read Publication using NB600-381B.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and 0.2% BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for V5 Epitope Tag Antibody [Biotin]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Background

The V5 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized V5 epitope represents amino acid residues 95 to 108, GKPIPNPLLGLDST, of RNA polymerase alpha subunit of simian virus 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V5 Epitope Tag Antibody (NB600-381B)(1)

We have publications tested in 1 application: ELISA.


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Secondary Antibodies

 

Isotype Controls

Additional V5 Epitope Tag Products

V5 Epitope Tag NB600-381B

Research Areas for V5 Epitope Tag Antibody (NB600-381B)

Find related products by research area.

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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