V5 Epitope Tag Antibody [Biotin]


Western Blot: V5 Epitope Tag Antibody [Biotin] [NB600-381B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP ...read more

Product Details

Reactivity Ec, NASpecies Glossary
Applications WB, ELISA
Please see the vial label for concentration. If unlisted please contact technical services.

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Available Conjugates
Catalog# & Conjugate Size Price

V5 Epitope Tag Antibody [Biotin] Summary

Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Biotin (A=N/A, E=N/A)
PBS and 0.2% BSA
0.05% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:10000-1:100000
  • ELISA 1:100-1:2000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 23785054)
Read Publication using NB600-381B.

Alternate Names for V5 Epitope Tag Antibody [Biotin]

  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 epitope tag


The V5 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized V5 epitope represents amino acid residues 95 to 108, GKPIPNPLLGLDST, of RNA polymerase alpha subunit of simian virus 5.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V5 Epitope Tag Antibody (NB600-381B)(1)

We have publications tested in 1 application: ELISA.

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies


Isotype Controls

Additional V5 Epitope Tag Antibody [Biotin] Products

V5 Epitope Tag NB600-381B

Research Areas for V5 Epitope Tag Antibody (NB600-381B)

Find related products by research area.

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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