694-GL) was serially diluted and captured by Mouse Anti-Human GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB6942) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GITR Ligand/TNFSF18 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 694-GL) was serially diluted and captured by Mouse Anti-Human GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB6942) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GITR Ligand/TNFSF18 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human GITR Ligand/TNFSF18 (Catalog # 694-GL) was serially diluted and captured by Mouse Anti-Human GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB6942) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GITR Ligand/TNFSF18 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4⁺T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox"> MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4⁺T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND₅₀ is typically 2-8 µg/mL." alt="In the presence of Mouse CD3e Monoclonal Anti-body (0.5 µg/mL, Catalog # MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4⁺T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

Immunoblot Buffer Group 1." class="big_lightbox"> Immunoblot Buffer Group 1." alt="Western blot shows lysates of A20 mouse B cell lymphoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB21772) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for GITR Ligand/TNFSF18 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). View our protocol for Staining Membrane-associated Proteins." alt="NS0 cell line transfected with either mouse GITR Ligand/TNFRSF18 (filled histogram) or irrelevant protein (open histogram) was stained with Rat Anti-Mouse GITR Ligand/TNFRSF18 Monoclonal Antibody (Catalog # MAB21773) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB4841) and a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (10 µg/mL, MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (2177-GL) stimulates proliferation in mouse CD4+T cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB21773). The ND₅₀ is typically 1.2-12 µg/mL." class="big_lightbox"> MAB4841) and a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (10 µg/mL, MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (2177-GL) stimulates proliferation in mouse CD4+T cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB21773). The ND₅₀ is typically 1.2-12 µg/mL." class="big_thumb" />

BAM6943) were stained with the indicated concentrations of Recombinant Human GITRL HA-tag Alexa Fluor® 488 (Catalog # AFG6987). " class="big_lightbox"> BAM6943) were stained with the indicated concentrations of Recombinant Human GITRL HA-tag Alexa Fluor® 488 (Catalog # AFG6987). " alt="Streptavidin coated beads conjugated to biotinylated Anti Human GITRL (BAM6943) were stained with the indicated concentrations of Recombinant Human GITRL HA-tag Alexa Fluor® 488 (Catalog # AFG6987). " class="big_thumb" />

F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). View our protocol for Staining Membrane-associated Proteins." alt="NS0 cell line transfected with either mouse GITR Ligand/TNFRSF18 (filled histogram) or irrelevant protein (open histogram) was stained with Rat Anti-Mouse GITR Ligand/TNFRSF18 Monoclonal Antibody (Catalog # MAB21774) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />