HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

795-P4) was serially diluted and captured by Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 795-P4) was serially diluted and captured by Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of mouse platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

595-P4) was serially diluted and captured by Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 595-P4) was serially diluted and captured by Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF005). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of mouse platelets. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

595-P4) was serially diluted and captured by Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 595-P4) was serially diluted and captured by Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

795-P4) was serially diluted and captured by Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 795-P4) was serially diluted and captured by Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human CXCL4/PF4 (Catalog # 795-P4) was serially diluted and captured by Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />