Mouse CXCL4/PF4 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse CXCL4/PF4 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Pf4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CXCL4/PF4 DuoSet ELISA, 15 Plate

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

CXCL4/PF4 is a secreted chemokine that is expressed by megakaryocytes and restrains the coagulation cascade. It is released by activated platelets and regulates Thrombin/Thrombomodulin complexes, activated Protein C (APC), Coagulation Factor Xa, and Fibrin. The anticoagulant heparin neutralizes CXCL4 procoagulant effects. Complexes of heparin and CXCL4 trigger antibody formation that causes the pathological syndrome HITT. Immunogenic complexes of CXCL4 with Apolipoprotein H contribute to antiphospholipid syndrome (APS). CXCL4 interferes with the proliferative and angiogenic functions of FGF-2 and VEGF. However, it can also exert proinflammatory and pro-atherogenic effects on monocytes, macrophages, and endothelial cells. CXCL4 may signal through CXCR3B in humans.

Publications for CXCL4/PF4 (DY595)(14)

Publications using DY595

• Becker, IC;Barrachina, MN;Lykins, J;Camacho, V;Stone, AP;Chua, BA;Signer, RAJ;Machlus, KR;Whiteheart, SW;Roweth, HG;Italiano, JE; Inhibition of RhoA-mediated secretory autophagy in megakaryocytes mitigates myelofibrosis in mice bioRxiv : the preprint server for biology 2024-12-05 [PMID: 39677616] (Mouse)
• M Salzmann, WC Schrottmai, JB Kral-Point, M Mussbacher, J Volz, B Hoesel, B Moser, S Bleichert, S Morava, B Nieswandt, JA Schmid, A Assinger Genetic platelet depletion is superior in platelet transfusion compared to current models Haematologica, 2019-09-19;0(0):. 2019-09-19 [PMID: 31537686] (Mouse)
• S Tariket, H Hamzeh-Cog, S Laradi, CA Arthaud, MA Eyraud, T Bourlet, P Berthelot, O Garraud, F Cognasse Evidence of CD40L/CD40 pathway involvement in experimental transfusion-related acute lung injury Sci Rep, 2019-08-29;9(1):12536. 2019-08-29 [PMID: 31467410] (Mouse)
• T Barwari, S Eminaga, U Mayr, R Lu, PC Armstrong, MV Chan, M Sahraei, M Fernández-, T Moreau, J Barallobre, M Lynch, X Yin, C Schulte, F Baig, R Pechlaner, SR Langley, A Zampetaki, P Santer, M Weger, R Plasenzott, M Schosserer, J Grillari, S Kiechl, J Willeit, AM Shah, C Ghevaert, TD Warner, C Fernández-, Y Suárez, M Mayr Inhibition of profibrotic microRNA-21 affects platelets and their releasate JCI Insight, 2018-11-02;3(21):. 2018-11-02 [PMID: 30385722] (Mouse)
• M Rami, R Guillamat-, P Rinne, M Salvermose, L Ring, M Bianchini, X Blanchet, RTA Megens, Y Döring, B Walzog, O Soehnlein, C Weber, A Faussner, S Steffens Chronic Intake of the Selective Serotonin Reuptake Inhibitor Fluoxetine Enhances Atherosclerosis Arterioscler. Thromb. Vasc. Biol., 2018-03-22;0(0):. 2018-03-22 [PMID: 29567680] (Mouse)
• C Daly, X Qian, C Castanaro, E Pasnikowsk, X Jiang, BR Thomson, SE Quaggin, N Papadopoul, Y Wei, JS Rudge, G Thurston, GD Yancopoulo, S Davis Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor Sci Rep, 2018-01-11;8(1):505. 2018-01-11 [PMID: 29323190] (Mouse)
• Dupuis-Maurin V, Brinza L, Baguet J, Plantamura E, Schicklin S, Chambion S, Macari C, Tomkowiak M, Deniaud E, Leverrier Y, Marvel J, Michallet M Overexpression of the transcription factor Sp1 activates the OAS-RNAse L-RIG-I pathway. PLoS ONE, 2015-03-04;10(3):e0118551. 2015-03-04 [PMID: 25738304] (Mouse)
• Lapchak PH, Ioannou A, Rani P The role of platelet factor 4 in local and remote tissue damage in a mouse model of mesenteric ischemia/reperfusion injury. PLoS ONE, 2012-07-06;7(7):e39934. 2012-07-06 [PMID: 22792197] (Mouse)
• Wang Y, Meng R, Hayes V, Fuentes R, Yu X, Abrams CS, Heijnen HF, Blobel GA, Marks MS, Poncz M Pleiotropic platelet defects in mice with disrupted FOG1-NuRD interaction. Blood, 2011-10-11;118(23):6183-91. 2011-10-11 [PMID: 21989988] (Mouse)
• Kelly-Spratt KS, Pitteri SJ, Gurley KE, Liggitt D, Chin A, Kennedy J, Wong CH, Zhang Q, Buson TB, Wang H, Hanash SM, Kemp CJ Plasma proteome profiles associated with inflammation, angiogenesis, and cancer. PLoS ONE, 2011-05-12;6(5):e19721. 2011-05-12 [PMID: 21589862] (Mouse)
• Morrell CN, Murata K, Swaim AM, Mason E, Martin TV, Thompson LE, Ballard M, Fox-Talbot K, Wasowska B, Baldwin WM In vivo platelet-endothelial cell interactions in response to major histocompatibility complex alloantibody. Circ. Res., 2008-02-22;102(7):777-85. 2008-02-22 [PMID: 18296616] (Mouse)
• de Jong EK, de Haas AH, Brouwer N, van Weering HR, Hensens M, Bechmann I, Pratley P, Wesseling E, Boddeke HW, Biber K Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3. J. Neurochem., 2008-02-01;105(5):1726-36. 2008-02-01 [PMID: 18248618] (Mouse)
• Woulfe DS, Lilliendahl JK, August S, Rauova L, Kowalska MA, Abrink M, Pejler G, White JG, Schick BP Serglycin proteoglycan deletion induces defects in platelet aggregation and thrombus formation in mice. Blood, 2007-12-19;111(7):3458-67. 2007-12-19 [PMID: 18094327] (Mouse)
• Schraw TD, Rutledge TW, Crawford GL, Bernstein AM, Kalen AL, Pessin JE, Whiteheart SW Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. Blood, 2003-05-08;102(5):1716-22. 2003-05-08 [PMID: 12738662] (Mouse)

FAQs for CXCL4/PF4 (DY595). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Pf4