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HAF017). A specific band was detected for Sonic Hedgehog/Shh at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (Catalog # 464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue). " class="big_lightbox" target="_blank">
1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively), and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (Catalog # 464-SH). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (MAB7566). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (MAB1195) followed by the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green). Cells were counterstained with DAPI (5748; blue)." class="big_lightbox" target="_blank">
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/images/antibody/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-1092025142349.jpg)
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Sonic_Hedgehog_MSHH00_ELISA_1032.jpg)
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize/elisa/Sonic_Hedgehog_DSHH00_ELISA_1118.jpg)
![N/A Sonic Hedgehog/Shh [Biotin]](http://images.novusbio.com/fullsize/dy1314-05_human-sonic-hedgehog-shh-n-terminus-duoset-elisa-5-plate-2782021132348.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/1845-sh_recombinant-human-sonic-hedgehog-shh-c24ii-n-terminus-sec-mals-1332024115815..jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_461-SH_474.jpg)
![Immunohistochemistry Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/images/antibody/Sonic_Hedgehog_AF464_Immunohistochemistry_17582.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-121220258452120.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-1212202582234.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-1212202582414.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-121220258141113.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-12122025846103.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-121220258463419.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-1212202581254.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-12122025932590.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-12122025932592.jpg)
![Western Blot Sonic Hedgehog/Shh Antibody [Unconjugated] - N-Terminus](http://images.novusbio.com/fullsize/af464_human-mouse-sonic-hedgehog-shh-n-term-affinity-purified-pab-western-blot-121220259321520.jpg)
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize/elisa/Sonic_Hedgehog_MSHH00_ELISA_323.jpg)
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize/elisa/Sonic_Hedgehog_DSHH00_ELISA_1116.jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-Page Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_464-SH_341.jpg)
![Cell Culture Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-cell-culture-105202415719..jpg)
![Cell Culture Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-cell-culture-105202415924..jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_1845-SH_52.jpg)
![SDS-Page Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_1845-SH_49.jpg)
![Mass Spectrometry Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_1845-SH_51.jpg)
![SDS-Page Sonic Hedgehog/Shh [Unconjugated]](http://images.novusbio.com/fullsize/protein/Sonic_Hedgehog_461-SH_463.jpg)
