| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33200 | |||
| IHC-Prediluted | NBP2-48225 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2756SS | |||
| Azide and BSA Free | NBP2-80688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98734-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87956-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49378-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08474 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08475 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26957 | |||
.png "Pan-Cytokeratin was detected in immersion fixed paraffin-embedded sections of human Breast Tumor using Mouse Anti-Human Cytokeratin, pan Monoclonal Antibody (Catalog # NBP2-29429) at 1:500 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.")
.png)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the AF647 version of this antibody.](http://images.novusbio.com/fullsize/nbp2-29429_mouse-monoclonal-cytokeratin-pan-antibody-ae-1-ae-3-imgenex-nbo-135a-20ug-17720249362612.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin [AE1 + AE3] Antibody NBP2-33200AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image from the AF488 version of this antibody.](http://images.novusbio.com/fullsize/nbp2-29429_mouse-monoclonal-cytokeratin-pan-antibody-ae-1-ae-3-imgenex-nbo-135a-20ug-17720241003217.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/nbp2-29429_mouse-monoclonal-cytokeratin-pan-antibody-ae-1-ae-3-imgenex-nbo-135a-20ug-1772024944230.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor cores showed a decreased staining and tumor stroma was mostly negative for Cytokeratin 1.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunohistochemistry-Paraffin-NB100-2756-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor cores showed a decreased staining and tumor stroma was mostly negative for Cytokeratin 1.")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor stroma was mostly negative for Cytokeratin 1. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunohistochemistry-Paraffin-NB100-2756-img0008.jpg)
![Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Western-Blot-NB100-2756-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Cytokeratin 1 antibody (LHK1) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunocytochemistry-Immunofluorescence-NB100-2756-img0005.jpg)
![Western Blot: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis using CK1 mouse mAb against truncated CK1 recombinant protein.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-3C10F7-Western-Blot-NB110-89450-img0004.jpg "Western Blot: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis using CK1 mouse mAb against truncated CK1 recombinant protein.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Cytokeratin 1 (3C10F7) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-3C10F7-Immunocytochemistry-Immunofluorescence-NB110-89450-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis of human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization using CK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-3C10F7-Immunohistochemistry-Paraffin-NB110-89450-img0005.jpg)
![Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Western-Blot-NBP2-67681-img0003.jpg "Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0006.jpg)
![Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Western-Blot-NBP2-67681-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (red) at 1/200 dilution and Vimentin (green) at 1/200 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0005.jpg)
![Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Western-Blot-NBP3-16883-img0007.jpg "Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Western-Blot-NBP3-16883-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-Paraffin-NBP3-16883-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-Paraffin-NBP3-16883-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded mouse skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-Paraffin-NBP3-16883-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in rat skin with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98734-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in rat skin with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in human hepatoma with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98734-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in human malignant melanoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98734-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in human skin with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98734-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in human stomach with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98734-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP2-98734] - Immunochemical staining Cytokeratin 1 in mouse skin with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98734-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP1-87956] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87956-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP1-87956] - Staining of human skin shows high expression.")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP1-87956] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87956-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody [NBP1-87956] - Staining in human skin and kidney tissues using anti-KRT1 antibody. Corresponding KRT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87956-img0006.jpg)
![Western Blot: Cytokeratin 1 Antibody [NBP2-49378] - Analysis in human skin tissue.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Western-Blot-NBP2-49378-img0002.jpg "Western Blot: Cytokeratin 1 Antibody [NBP2-49378] - Analysis in human skin tissue.")
![Immunohistochemistry: Cytokeratin 1 Antibody [NBP2-49378] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-Immunohistochemistry-NBP2-49378-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NBP3-07775] - Formalin-fixed, paraffin-embedded human Cervix stained with Cytokeratin 1 Mouse Monoclonal Antibody (LHK1).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunohistochemistry-Paraffin-NBP3-07775-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NBP3-07775] - Formalin-fixed, paraffin-embedded human Cervix stained with Cytokeratin 1 Mouse Monoclonal Antibody (LHK1).")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NBP3-07775] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 1 Mouse Monoclonal Antibody (LHK1).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunohistochemistry-Paraffin-NBP3-07775-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) [NBP3-07776] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-KRT1-1840-Immunohistochemistry-Paraffin-NBP3-07776-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) [NBP3-07776] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) [NBP3-07776] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-KRT1-1840-Immunohistochemistry-Paraffin-NBP3-07776-img0001.jpg)
![SDS-Page: Recombinant Human Cytokeratin 1 Protein [H00003848-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Cytokeratin-1-Protein-SDS-Page-H00003848-Q01-img0002.jpg "SDS-Page: Recombinant Human Cytokeratin 1 Protein [H00003848-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
