| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56145SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88301-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59723SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56173SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56179SS | |||
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0003.jpg "Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404] - Staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4)](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunocytochemistry-Immunofluorescence-NB100-404-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade diluted at 1:500.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - NB100-404 was used at 1:1000 dilution for Western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody (13B3) (NB100-147) was used as a loading control.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Western-Blot-NB100-404-img0004.jpg)
![Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404] - 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Immunoprecipitation-NB100-404-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - DAG-induced DNA damage is repaired by homologous recombination. A549 cells were transfected with either negative control (C) or three BRCA1-targeting siRNAs (B1, siBRCA1-2; B2, siBRCA1-15; or B3, siBRCA1-17) for 24 h. Cells were then seeded in 96-well culture plates and treated with different concentrations of VAL-083 (0, 100 nM, 500 nM, 1 uM, 1.5 uM, 2.5 uM, 5 uM, 10 uM, 25 uM, 50 uM, 100 uM, and 200 uM) for 5 days. Following the treatment, crystal violet assay was performed to detect the absorbance at 560 nm wavelength. Cell lysates from the control or BRCA1-knockdown cells were analyzed by western blot with antibody against BRCA1, and GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1069-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockdown-Validated-NB100-404-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody, diluted at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRCA1-Antibody-6B4-Knockout-Validated-NB100-404-img0015.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (NB100-404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nb100-404_mouse-monoclonal-brca1-antibody-6b4-91020242091412.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0011.jpg "Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.")
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0008.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0002.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0009.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0003.jpg)
![Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Sandwich-ELISA-H00055743-M01-img0010.jpg)
![Western Blot: Livin Antibody [NB100-56145] - Western blot analysis of livin isoforms expression in three of four paired samples of tumors (2 ACA and 1 ACC) and adjacent NAG that are showed also at mRNA levels. Whole cell lysate SK-MEL 28 was used as positive control and B-actin was used as internal standard. (D) Quantitative analysis of WB bands of all 15 paired samples of tumors and corresponding NAG (p < 0.0001 per trend). Each bar in the histograms represents the mean of the ratio livin A or livin B signal to B-actin signal. Statistical analysis by Kruskall-Wallis test and Mann-Whitney U test. N.s. = p not significant. Image collected and cropped by Citeab from the following publication (Livin/BIRC7 expression as malignancy marker in adrenocortical tumors. Oncotarget (2017) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Livin-Antibody-Western-Blot-NB100-56145-img0008.jpg "Western Blot: Livin Antibody [NB100-56145] - Western blot analysis of livin isoforms expression in three of four paired samples of tumors (2 ACA and 1 ACC) and adjacent NAG that are showed also at mRNA levels. Whole cell lysate SK-MEL 28 was used as positive control and B-actin was used as internal standard. (D) Quantitative analysis of WB bands of all 15 paired samples of tumors and corresponding NAG (p < 0.0001 per trend). Each bar in the histograms represents the mean of the ratio livin A or livin B signal to B-actin signal. Statistical analysis by Kruskall-Wallis test and Mann-Whitney U test. N.s. = p not significant. Image collected and cropped by Citeab from the following publication (Livin/BIRC7 expression as malignancy marker in adrenocortical tumors. Oncotarget (2017) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Livin Antibody [NB100-56145] - Livin staining in normal adrenal glandLivin staining in normal adrenal gland (A). It is possible to recognize the three different zone of the adrenal gland: the zona glomerulosa (ZG), the zona fasciculata (ZF) and the zona reticularis (ZR). The Figures (B) and (C) are 20A- enlarged and detailed images of the 10A- ZG, ZF and ZR, respectively. Scale bar: 100 i1/4m. Image collected and cropped by Citeab from the following publication (Livin/BIRC7 expression as malignancy marker in adrenocortical tumors. Oncotarget (2017) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Livin-Antibody-Immunohistochemistry-Paraffin-NB100-56145-img0007.jpg)
![Western Blot: Livin Antibody [NB100-56145] - Analysis of full-length, recombinant human Livin using this antibody.](http://images.novusbio.com/fullsize/Livin-Antibody-Western-Blot-NB100-56145-img0004.jpg)
![Western Blot: Livin Antibody [NB100-56145] - Effect of livin overexpression on adrenocortical cell line NCI-H295R in vitroRelative livin mRNA levels. WB analysis showed clearly higher livin i+- and i2 protein levels and a light decrease of cleaved caspase 3 protein levels at both 48 and 72 hours. Image collected and cropped by Citeab from the following publication (Livin/BIRC7 expression as malignancy marker in adrenocortical tumors. Oncotarget (2017) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Livin-Antibody-Western-Blot-NB100-56145-img0006.jpg)
![Immunohistochemistry: Livin Antibody [NB100-56145] - Immunohistochemical analysis of Livin in formalin-fixed, paraffin embedded human tonsil (secondary nodule) using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Livin-Antibody-Immunohistochemistry-NB100-56145-img0002.jpg)
![Immunohistochemistry-Paraffin: Livin Antibody [NB100-56145] - Livin Antibody [NB100-56145] - Analysis of Livin in Bouin's-fixed, paraffin-embedded E13.5 mouse embryo (13.5 days post coitum) Bouin's using this antibody at 1:2000. The mother was perfused prior to embryo harvesting. A) brain overview B) brain C) spinal cord. D) liver and abdominal wall. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Livin-Antibody-Immunohistochemistry-Paraffin-NB100-56145-img0005.jpg)
![Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Western-Blot-NBP1-41185-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.")
![<b>Genetic Strategies Validation. </b>Chromatin Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Chromatin-Immunoprecipitation-NBP1-41185-img0009.jpg)
![Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Western-Blot-NBP1-41185-img0012.jpg)
![Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Immunoprecipitation-NBP1-41185-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-41185_mouse-monoclonal-brca1-antibody-17f8-1610202419414553.jpg)
![Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-97371-img0001.jpg "Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97371-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97371-img0002.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0025.jpg "Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Immunofluorescence Microscopy of RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue.Fixation: 4% PFA. Antigen retrieval: Heat.Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT.Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT.Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0019.jpg)
![Immunohistochemistry: RFP Antibody [NBP1-69962] - Immunohistochemistry of RFP antibody.Tissue: Mouse gut tissue in tomato transgenic mice.Fixation: formalin fixed paraffin embedded. Antigen retrieval: heat 5 min at high temp in 1X rodent decloaker. Primary antibody: RFP antibody at 1:200 for 1 h 30 min at RT.Secondary Antibody: HRP anti-rabbit.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunohistochemistry-NBP1-69962-img0026.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye 800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0008.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: RFP. Lane 2: Human IgG. Lane 3: Goat IgG. Lane 4: Mouse IgG. Load: 50ng per lane.Primary antibody: RFP antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase conjugated rabbit secondary antibody at 1:40,000 for 30 min at RT.Block Blocking Buffer for 30 min at RT.Predicted/Observed size: 27 kDa, 30 kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0021.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Marker.Lane 2: 50ng of RFP. Primary Antibody: Anti-RFP at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30mins at RT. Block: BlockOut Universal blocking buffer. Expect ~27kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0022.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Protein. Lane 11: Green Fluorescent Protein. Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein. Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: RFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 30 kDa for RFP.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0023.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: RFP/HEK293T WCL [0.05/10ug] [+]. Lane 3: HEK293T Whole Cell Lysate [-]. Lane 4: RFP/NIH/3T3 Whole Cell Lysate [0.05/10ug] [+]. Lane 5: NIH/3T3 Whole Cell Lysate [-]. Lane 6: RFP/PC-12 Whole Cell Lysate [0.05/10ug] [+]. Lane 7: PC-12 Whole Cell Lysate [-]. Primary Antibody: Anti-RFP at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase at 1:70,000 at RT for 30 mins. Predicted/Observed MW: ~27kDa. Exposure: 2 sec.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: HopERCre/+; R26Tom/+ mice. Fixation: 0.5% PFA. Antigen retrieval: Tamoxifen. Primary antibody: RFP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: Hop-derived cells in the hair follicle, labeled in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse lung tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: SPC+ cells are RFP positive in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0003.jpg)
![CyTOF-ready: RFP Antibody [NBP1-69962] - Analysis of RFP antibody on human tissue. RFP-expressing cells (red), nuclei (blue). Scale, 50 um. Image from verified customer review](http://images.novusbio.com/fullsize/RFP-Antibody-CyTOF-ready-NBP1-69962-img0016.jpg)
![ELISA: RFP Antibody [NBP1-69962] - ELISA Results of RFP antibody tested against purified RFP protein. Each well was coated in duplicate with 1.0 ug of RFP [red], Human IgG [green], Mouse IgG [blue], Rat IgG [purple]. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% Fish Gel in PBS Blocking Buffer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate. Results show RFP titer >75,000, Human, Mouse, Rat IgG titers <100.](http://images.novusbio.com/fullsize/RFP-Antibody-ELISA-NBP1-69962-img0027.jpg)
![Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB110-59723-img0002.jpg "Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-EGR2 Antibody NB110-59723 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-59723-img0007.jpg)
![Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of EGR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Paraffin-NB110-59723-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunofluorescence-NB110-59723-img0006.jpg)
![Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of RNF111 expression in transfected 293T cell line by RNF111 monoclonal antibody (M05), clone 1C4. Lane 1: RNF111 transfected lysatE (107.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RNF111-Antibody-1C4-Western-Blot-H00054778-M05-img0005.jpg "Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of RNF111 expression in transfected 293T cell line by RNF111 monoclonal antibody (M05), clone 1C4. Lane 1: RNF111 transfected lysatE (107.8 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of monoclonal antibody to RNF111 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RNF111-Antibody-1C4-Immunocytochemistry-Immunofluorescence-H00054778-M05-img0003.jpg)
![Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Western blot analysis of RNF111 over-expressed 293 cell line, cotransfected with RNF111 Validated Chimera RNAi or non-transfected control. Blot probed with H00054778-M05. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/RNF111-Antibody-1C4-Western-Blot-H00054778-M05-img0002.jpg)
![ELISA: RNF111 Antibody (1C4) [H00054778-M05] - Detection limit for recombinant GST tagged RNF111 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RNF111-Antibody-1C4-ELISA-H00054778-M05-img0006.jpg)
![Western Blot: TRAF-2 Antibody [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NB100-56173-img0008.jpg "Western Blot: TRAF-2 Antibody [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NB100-56173] - Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfectionMCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies. Image collected and cropped by Citeab from the following publication (A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget (2011) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56173-img0009.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NB100-56173-img0007.jpg)
![Western Blot: TRAF-2 Antibody [NB100-56173] - Detection of TRAF-2 protein in various tumor cell lines using this antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NB100-56173-img0005.jpg)
![Immunohistochemistry: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human melanoma tissue section stained for TRAF2 at 1:2000. A1 is a higher magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-NB100-56173-img0006.jpg)
![Western Blot: TRAF-2 Antibody [NB100-56173] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated & HN30 cells transfected with pcDNA 3.0 NSP 5a3a & pcDNA 3.1/CT-GFP for 3 daysNT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a, & f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56173_rabbit-polyclonal-traf-2-antibody-31020241552526.jpg)
![Western Blot: TRAF-6 Antibody [NB100-56179] - Analysis of TRAF-6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-6 degradation fragments.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NB100-56179-img0011.jpg "Western Blot: TRAF-6 Antibody [NB100-56179] - Analysis of TRAF-6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-6 degradation fragments.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NB100-56179] - Stained for TRAF6 using this antibody at 1:2000. Hematoxylin-eosin counterstain. A, mouse spleen. B, mouse thymus.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NB100-56179-img0008.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NB100-56179] - 1:2000. Analysis Hematoxylin-eosin counterstain. A, mouse lymph node. B, mouse bone marrow.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NB100-56179-img0007.jpg)
![Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TRIM32-Antibody-2E5-Western-Blot-H00022954-M09-img0006.jpg "Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of monoclonal antibody to TRIM32 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/TRIM32-Antibody-2E5-Immunocytochemistry-Immunofluorescence-H00022954-M09-img0004.jpg)
![Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi or non-transfected control. Blot probed with H00022954-M09. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/TRIM32-Antibody-2E5-Western-Blot-H00022954-M09-img0002.jpg)
![ELISA: TRIM32 Antibody (2E5) [H00022954-M09] - Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRIM32-Antibody-2E5-Sandwich-ELISA-H00022954-M09-img0005.jpg)