| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35170-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38578-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92968-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38464-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47921-0.1mg | |||
| IHC-Prediluted | NBP2-48127 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21529-50ul | |||
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202415143627.jpg "Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Human esophagus using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202414163033.jpg)
![Western Blot: RAC2 Antibody [NBP3-35170] - Western blot analysis of various lysates, using RAC2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-91220241519516.jpg)
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Mouse stomach using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202415121394.jpg)
![Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Western-Blot-NBP3-15627-img0009.jpg "Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Knockout-Validated-NBP3-15627-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100 x.](http://images.novusbio.com/fullsize/nbp3-15627_rabbit-cyclin-d1-mab-7c8z4-immunocytochemistry-immunofluorescence-2092023105125.jpg)
![Immunocytochemistry/ Immunofluorescence: CACYBP Antibody [NBP3-38578] - Immunofluorescence analysis of HeLa cells using [KO Validated] CACYBP Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38578_rabbit-cacybp-pab-101220241243944.jpg "Immunocytochemistry/ Immunofluorescence: CACYBP Antibody [NBP3-38578] - Immunofluorescence analysis of HeLa cells using [KO Validated] CACYBP Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: CACYBP Antibody [NBP3-38578] - Western blot analysis of various lysates, using [KO Validated] CACYBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38578_rabbit-cacybp-pab-101220241214482.jpg)
![Immunocytochemistry/ Immunofluorescence: CACYBP Antibody [NBP3-38578] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] CACYBP Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38578_rabbit-cacybp-pab-10122024120676.jpg)
![Western Blot: CACYBP Antibody [NBP3-38578] - Western blot analysis of lysates from wild type(WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38578_rabbit-cacybp-pab-101220241252859.jpg)
![Western Blot: CACYBP Antibody [NBP3-38578] - Western blot analysis of various lysates using [KO Validated] CACYBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38578_rabbit-cacybp-pab-101220241233899.jpg)
![Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Western-Blot-NBP2-92968-img0005.jpg "Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% non](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Knockout-Validated-NBP2-92968-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968] - Immunofluorescence analysis of HeLa cells using [KO Validated] Casein Kinase 1 epsilon Rabbit pAb . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92968_rabbit-polyclonal-casein-kinase-1-epsilon-antibody-422025143182.jpg%3Ft=1709606037)
![Immunocytochemistry/ Immunofluorescence: FBXW11 Antibody [NBP3-38464] - Immunofluorescence analysis of A-431 cells using FBXW11 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38464_rabbit-fbxw11-pab-10122024120664.jpg "Immunocytochemistry/ Immunofluorescence: FBXW11 Antibody [NBP3-38464] - Immunofluorescence analysis of A-431 cells using FBXW11 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: FBXW11 Antibody [NBP3-38464] - Western blot analysis of lysates from A-431 cells, using FBXW11 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38464_rabbit-fbxw11-pab-101220241214439.jpg)
![Immunocytochemistry/ Immunofluorescence: FBXW11 Antibody [NBP3-38464] - Immunofluorescence analysis of PC-12 cells using FBXW11 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38464_rabbit-fbxw11-pab-101220241252818.jpg)
![Immunocytochemistry/ Immunofluorescence: FBXW11 Antibody [NBP3-38464] - Immunofluorescence analysis of HeLa cells using FBXW11 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38464_rabbit-fbxw11-pab-1012202411552819.jpg)
![Immunocytochemistry/ Immunofluorescence: FBXW11 Antibody [NBP3-38464] - Immunofluorescence analysis of NIH/3T3 cells using FBXW11 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38464_rabbit-fbxw11-pab-101220241243952.jpg)
![Immunoprecipitation: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32107 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32107 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32107 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32107 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-2682024158264.jpg "Immunoprecipitation: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32107 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32107 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32107 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32107 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL")
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Immunocytochemistry analysis of HCT 116 cells labeling Casein Kinase 1 alpha with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-29202410484857.jpg)
![Flow Cytometry: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Flow cytometric analysis of HCT 116 cells labeling Casein Kinase 1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32107, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-29202416372134.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (PSH04-46) [NBP3-32107] - Western blot analysis of Casein Kinase 1 alpha on different lysates with Rabbit anti-Casein Kinase 1 alpha antibody (NBP3-32107) at 1/2,000 dilution.Lane 1: HCT 116 cell lysate (20 µg/Lane)Lane 2: HeLa cell lysate (20 µg/Lane)Lane 3: HEK-293 cell lysate (20 µg/Lane)Lane 4: MCF7 cell lysate (20 µg/Lane)Lane 5: Jurkat cell lysate (20 µg/Lane)Lane 6: NIH/3T3 cell lysate (20 µg/Lane)Lane 7: RAW264.7 cell lysate (20 µg/Lane)Lane 8: PC-12 cell lysate (20 µg/Lane)Lane 9: Mouse brain tissue lysate (40 µg/Lane)Lane 10: Rat brain tissue lysate (40 µg/Lane)Predicted band size: 39 kDaObserved band size: 34 kDaExposure time: 15 seconds; ECL;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32107_rabbit-casein-kinase-1-alpha-mab-psh04-46-3920248453528.jpg)
![Immunohistochemistry: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of colona carcinoma](http://images.novusbio.com/fullsize/Wnt-8a-Antibody-Immunohistochemistry-NBP3-14523-img0006.jpg "Immunohistochemistry: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of colona carcinoma")
![Immunohistochemistry-Paraffin: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin, melanoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Wnt-8a-Antibody-Immunohistochemistry-Paraffin-NBP3-14523-img0001.jpg)
![Immunohistochemistry-Paraffin: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of formalin-fixed, paraffin-embedded human breast after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Wnt-8a-Antibody-Immunohistochemistry-Paraffin-NBP3-14523-img0002.jpg)
![Immunohistochemistry-Paraffin: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Wnt-8a-Antibody-Immunohistochemistry-Paraffin-NBP3-14523-img0003.jpg)
![Immunohistochemistry-Paraffin: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, non-small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Wnt-8a-Antibody-Immunohistochemistry-Paraffin-NBP3-14523-img0004.jpg)
![Immunohistochemistry: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of brain, caudate, neurons and glia](http://images.novusbio.com/fullsize/Wnt-8a-Antibody-Immunohistochemistry-NBP3-14523-img0005.jpg)
![Immunohistochemistry: Wnt-8a Antibody [NBP3-14523] - Immunohistochemistry of thalamus, neurons and glia](http://images.novusbio.com/fullsize/Wnt-8a Antibody-Immunohistochemistry-NBP3-14523-img0007.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-278202416331227.jpg "Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32350) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29820241435263.jpg)
![Immunocytochemistry/ Immunofluorescence: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunocytochemistry analysis of U-87 MG cells labeling FRA-1/FOSL1 with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202410484854.jpg)
![Flow Cytometry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Flow cytometric analysis of U-87 MG cells labeling FRA-1/FOSL1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32350, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202416453260.jpg)
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - Mouse fore brain. RAC2 antibody [N1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-33449-img0004.jpg "Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - Mouse fore brain. RAC2 antibody [N1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - NCI-N87 xenograft, using RAC2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-33449-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: RAC2 Antibody [NBP1-33449] - RAC2 antibody [N1C3] detects RAC2 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAC2 stained by RAC2 antibody [N1C3] (NBP1-33449) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33449_rabbit-polyclonal-rac2-antibody-910202420122071.jpg)
![Immunohistochemistry-Paraffin: RAC2 Antibody [NBP1-33449] - RAC2 antibody [N1C3] detects RAC2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.RAC2 stained by RAC2 antibody [N1C3] (NBP1-33449) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33449_rabbit-polyclonal-rac2-antibody-91020242011452.jpg)
![Western Blot: RAC2 Antibody [NBP1-33449] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAC2 antibody [N1C3] (NBP1-33449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33449_rabbit-polyclonal-rac2-antibody-910202420104415.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0022.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0016.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Mantle Cell Lymphoma stained with Cyclin D1 Monoclonal Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Immunohistochemistry-Paraffin-NBP2-44586-img0003.jpg "Immunohistochemistry-Paraffin: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Mantle Cell Lymphoma stained with Cyclin D1 Monoclonal Antibody (CCND1/809).")
![Flow Cytometry: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Cyclin D1 Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Flow-Cytometry-NBP2-44586-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Bladder Carcinoma stained with Cyclin D1 Monoclonal Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Immunohistochemistry-Paraffin-NBP2-44586-img0002.jpg)
![Flow Cytometry: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Cyclin D1 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Cyclin D1 Monoclonal Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Flow-Cytometry-NBP2-44586-img0001.jpg)
![Flow Cytometry: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Flow Cytometric analysis of human Cyclin D1 on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Flow-Cytometry-NBP2-44586-img0005.jpg)
![Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-572023156479.jpg "Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.")
![Immunohistochemistry: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-5720231558484.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-6720231051264.jpg)