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![Immunohistochemistry: MMP-7 Antibody [NBP1-99123] - MMP7 antibody was tested in human breast cancer using DAB with hematoxylin counterstain. The image on the right had the antibody blocked by preincubation with immunizing peptide.](http://images.novusbio.com/fullsize/MMP-7-Antibody-Immunohistochemistry-NBP1-99123-img0002.jpg "Immunohistochemistry: MMP-7 Antibody [NBP1-99123] - MMP7 antibody was tested in human breast cancer using DAB with hematoxylin counterstain. The image on the right had the antibody blocked by preincubation with immunizing peptide.")
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody detects GSK-3 beta protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK-3 beta stained by GSK-3 beta antibody (NBP3-13679) diluted at 1:500.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunocytochemistry-Immunofluorescence-NBP3-13679-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0003.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0001.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0002.jpg)
![Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Wnt9a stained by Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25757_rabbit-wnt-9a-mab-hl2422-azide-and-bsa-free-2120249374984.jpg "Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Wnt9a stained by Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25757_rabbit-wnt-9a-mab-hl2422-azide-and-bsa-free-2120249393529.jpg)
![Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25757_rabbit-wnt-9a-mab-hl2422-azide-and-bsa-free-2120249413324.jpg)
![Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Wnt9a stained by Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25757_rabbit-wnt-9a-mab-hl2422-azide-and-bsa-free-2120249355577.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Various whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25757_rabbit-wnt-9a-mab-hl2422-azide-and-bsa-free-2120249362794.jpg)
![Wnt-9a antibody [HL2422] detects Wnt-9a protein by immunofluorescent analysis.Sample: T-47D cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Wnt-9a stained by Wnt-9a antibody [HL2422] (NBP3-25757) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25757_rabbit-wnt-9a-mab-hl2422-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518434421.jpg)
![Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.](http://images.novusbio.com/fullsize/GRG-Groucho-homolog-Antibody-Western-Blot-NBP1-76645-img0005.jpg "Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunofluorescence of GRG (Groucho homolog) in Hela cells with GRG (Groucho homolog) antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76645_rabbit-polyclonal-grg-groucho-homolog-antibody-2711202419475890.jpg)
![Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunocytochemistry of GRG (Groucho homolog) in HeLa cells with GRG (Groucho homolog) antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76645_rabbit-polyclonal-grg-groucho-homolog-antibody-271120242022923.jpg)
![Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NB100-41374-img0001.jpg "Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0003.jpg)
![Flow Cytometry: LEF1 Antibody [NB100-41374] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-Flow-Cytometry-NB100-41374-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0002.jpg)
![Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0007.jpg "Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate")
![Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-3A6-Immunohistochemistry-Paraffin-NBP1-19050-img0004.jpg)
![Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-7Z6Q1-Immunocytochemistry-Immunofluorescence-NBP3-16352-img0002.jpg "Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunohistochemistry of paraffin-embedded human colon using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-7Z6Q1-Immunohistochemistry-Paraffin-NBP3-16352-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of U-2 OS cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-5220251215490.jpg)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of NIH/3T3 cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-5220251242529.jpg)
![Western Blot: sFRP-4 Antibody (7Z6Q1) [sFRP-4] - Western blot analysis of various lysates using sFRP-4 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-52202517192946.jpg)
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-35369] - Immunofluorescence analysis of C6 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411381284.jpg "Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-35369] - Immunofluorescence analysis of C6 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PKC beta Antibody [NBP3-35369] - Western blot analysis of various lysates using PKC beta Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411334057.jpg)
![Immunohistochemistry: PKC beta Antibody [NBP3-35369] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411433423.jpg)
![Immunohistochemistry: PKC beta Antibody [NBP3-35369] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411381212.jpg)
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-35369] - Immunofluorescence analysis of NIH-3T3 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-3112025173159.jpg)
![Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of various lysates, using Smad3 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15672_rabbit-smad3-mab-1t1q9-52202517311042.jpg "Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of various lysates, using Smad3 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunohistochemistry: Smad3 Antibody (1T1Q9) [Smad3] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] Smad3 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15672_rabbit-smad3-mab-1t1q9-5220251733554.jpg)
![Immunohistochemistry: Smad3 Antibody (1T1Q9) [Smad3] - Immunohistochemistry analysis of paraffin-embedded Human testis using [KO Validated] Smad3 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15672_rabbit-smad3-mab-1t1q9-52202517304924.jpg)
![Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of lysates from Rat testis, using Smad3 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15672_rabbit-smad3-mab-1t1q9-52202517352711.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of lysates from wild type(WT) and Smad3 Rabbit mAb knockout (KO) HCT116(KO) cells, using Smad3 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15672_rabbit-smad3-mab-1t1q9-52202517315550.jpg)
![Western Blot: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Western blot analysis of extracts of various cell lines, using RUVBL1 Rabbit mAb (NBP3-16583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-7Q2U8-Western-Blot-NBP3-16583-img0002.jpg "Western Blot: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Western blot analysis of extracts of various cell lines, using RUVBL1 Rabbit mAb (NBP3-16583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Immunohistochemistry of paraffin-embedded mouse testis using RUVBL1 Rabbit mAb (NBP3-16583) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-7Q2U8-Immunohistochemistry-Paraffin-NBP3-16583-img0001.jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [NBP3-16583] -Analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-chromatin-immunoprecipitation-78202411528..jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-522025126537.jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [RUVBL1] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-52202517162323.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0004.jpg "Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad2 Rabbit mAb (NBP3-15671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunocytochemistry-Immunofluorescence-NBP3-15671-img0002.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of various cell lines, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0003.jpg)
![Immunoprecipitation: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Smad2 antibody (NBP3-15671). Western blot was performed from the immunoprecipitate using Smad2 antibody (NBP3-15671) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunoprecipitation-NBP3-15671-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody (2E3D8) [Smad2] - Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells using [KD Validated] Smad2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-52202517304995.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (2E3D8) [Smad2] - Confocal imaging of HeLa cells using [KD Validated] Smad2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-522025173455.jpg)
![Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Western-Blot-NBP3-16536-img0005.jpg "Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunohistochemistry-Paraffin-NBP3-16536-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of NIH-3T3 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0003.jpg)