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![Western Blot: PPP2CB Antibody [NBP2-94074] - Analysis of extracts of various cell lines, using PPP2CB at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Western-Blot-NBP2-94074-img0005.jpg "Western Blot: PPP2CB Antibody [NBP2-94074] - Analysis of extracts of various cell lines, using PPP2CB at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: PPP2CB Antibody [NBP2-94074] - Analysis of U-2 OS cells using PPP2CB at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94074-img0001.jpg)
![Immunohistochemistry-Paraffin: PPP2CB Antibody [NBP2-94074] - Paraffin-embedded rat kidney using PPP2CB .](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Immunohistochemistry-Paraffin-NBP2-94074-img0004.jpg)
![Immunohistochemistry-Paraffin: PPP2CB Antibody [NBP2-94074] - Paraffin-embedded human colon carcinoma using PPP2CB .](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Immunohistochemistry-Paraffin-NBP2-94074-img0002.jpg)
![Immunohistochemistry-Paraffin: PPP2CB Antibody [NBP2-94074] - Paraffin-embedded mouse kidney using PPP2CB .](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Immunohistochemistry-Paraffin-NBP2-94074-img0003.jpg)
![Western Blot: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Western blot analysis of various lysates, using Dishevelled-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp2-92847_rabbit-polyclonal-dishevelled-1-antibody-42202514251863.jpg "Western Blot: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Western blot analysis of various lysates, using Dishevelled-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Immunohistochemistry-Paraffin: Dishevelled-1 Antibody [NBP2-92847] - Paraffin-embedded rat testis using Dishevelled-1 .](http://images.novusbio.com/fullsize/Dishevelled-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92847-img0003.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-1 Antibody [NBP2-92847] - Paraffin-embedded mouse testis using Dishevelled-1 .](http://images.novusbio.com/fullsize/Dishevelled-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92847-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Immunofluorescence analysis of C6 cells using Dishevelled-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92847_rabbit-polyclonal-dishevelled-1-antibody-4220251430453.jpg)
![Immunocytochemistry/ Immunofluorescence: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Immunofluorescence analysis of NIH-3T3 cells using Dishevelled-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92847_rabbit-polyclonal-dishevelled-1-antibody-42202514264410.jpg)
![Western Blot: SIAH1 Antibody [NBP2-93864] - Analysis of extracts of mouse kidney, using SIAH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Western-Blot-NBP2-93864-img0005.jpg "Western Blot: SIAH1 Antibody [NBP2-93864] - Analysis of extracts of mouse kidney, using SIAH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NBP2-93864] - Immunofluorescence analysis of L929 cells using SIAH1 Rabbit pAb (NBP2-93864) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93864-img0006.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Rat brain using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0004.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Human colon carcinoma using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0002.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Mouse spleen using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0003.jpg)
![Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-298202414304156.jpg "Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunocytochemistry analysis of Neuro-2a cells labeling Cyclin D1 with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cyclin D1 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202410484833.jpg)
![Flow Cytometry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Flow cytometric analysis of MCF7 cells labeling Cyclin D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32236, red) at 1/5,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202416453255.jpg)
![Western Blot: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Western blot analysis of Cyclin D1 on different lysates with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/5,000 dilution.Lane 1: MCF7 cell lysateLane 2: K-562 cell lysate (negative)Lane 3: A431 cell lysateLane 4: Neuro-2a cell lysateLane 5: NIH/3T3 cell lysateLane 6: C6 cell lysateLane 7: SH-SY5Y cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 34 kDaObserved band size: 35 kDaExposure time: 20 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-3920248485020.jpg)
![Western Blot: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Western blot analysis of Dishevelled-2 on different lysates with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: NCI-H1299 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 90 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32269) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-2782024163169.jpg "Western Blot: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Western blot analysis of Dishevelled-2 on different lysates with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: NCI-H1299 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 90 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32269) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32269) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-298202414333677.jpg)
![Immunocytochemistry/ Immunofluorescence: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Immunocytochemistry analysis of HeLa cells labeling Dishevelled-2 with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-2920241192360.jpg)
![Flow Cytometry: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Flow cytometric analysis of HeLa cells labeling Dishevelled-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32269, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-392024884683.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Anti-FZD9 / Frizzled 9 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0006.jpg "Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Anti-FZD9 / Frizzled 9 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Analysis of anti-FZD9 / Frizzled 9 antibody with brain, Purkinje neurons at 13 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Analysis of anti-FZD9 / Frizzled 9 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0003.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Anti-FZD9 / Frizzled 9 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0005.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Anti-FZD9 / Frizzled 9 antibody IHC of human brain, Purkinje neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0007.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0008.jpg "Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with transfection control at 6 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with transfection control.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0001.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with transfected cells expressing Frizzled-6 at 6 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Analysis of anti-Frizzled-6 antibody with human pancreas.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0006.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4482] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NLS4482-img0007.jpg)
![Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-288202412442938.png "Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29820241426262.png)
![Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-2920241114810.png)
![Flow Cytometry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Flow cytometric analysis of MCF-7 cells labeling PPP3CB. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32848, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29202416372124.png)
![Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Lung, non small-cell carcinoma](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Immunohistochemistry-Paraffin-NLS4900-img0006.jpg "Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Lung, non small-cell carcinoma")
![Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Analysis of anti-FZD7 / Frizzled 7 antibody with human heart, cardiac myocytes.](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Immunohistochemistry-Paraffin-NLS4900-img0001.jpg)
![Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Analysis of anti-FZD7 / Frizzled 7 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Immunohistochemistry-Paraffin-NLS4900-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Immunohistochemistry-Paraffin-NLS4900-img0003.jpg)
![Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Immunohistochemistry-Paraffin-NLS4900-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-7 Antibody [NLS4900] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/Frizzled-7-Antibody-Immunohistochemistry-Paraffin-NLS4900-img0005.jpg)
![Western Blot: NLK Antibody [NBP1-32264] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NLK-Antibody-Western-Blot-NBP1-32264-img0006.jpg "Western Blot: NLK Antibody [NBP1-32264] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: NLK Antibody [NBP1-32264] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NLK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32264-img0002.jpg)
![Immunohistochemistry-Paraffin: NLK Antibody [NBP1-32264] - Paraffin-embedded mouse ovary. NLK antibody [N3C3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/NLK-Antibody-Immunohistochemistry-Paraffin-NBP1-32264-img0005.jpg)
![Western Blot: NLK Antibody [NBP1-32264] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NLK-Antibody-Western-Blot-NBP1-32264-img0003.jpg)
![Western Blot: NLK Antibody [NBP1-32264] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE NLK antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NLK-Antibody-Western-Blot-NBP1-32264-img0004.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-27147-img0002.jpg "Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-27147] - Immunohistochemical analysis of Rvb1 in formalin-fixed, paraffin-embedded mouse testis tissue using NBP2-27147 at 5 ug/ml.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-27147-img0003.jpg)