| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41306-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37964-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35347-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89621-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82009-0.025ml | |||
![Western Blot: Wnt-5b Antibody [NBP2-41306] - Western blot analysis of WNT-5b in Jurkat cell lysate with WNT-5b antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Wnt-5b-Antibody-Western-Blot-NBP2-41306-img0001.jpg "Western Blot: Wnt-5b Antibody [NBP2-41306] - Western blot analysis of WNT-5b in Jurkat cell lysate with WNT-5b antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: Wnt-5b Antibody - BSA Free [NBP2-41306] - Immunohistochemistry of Wnt-5b in rat colon tissue with Wnt-5b antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41306_rabbit-polyclonal-wnt-5b-antibody-2711202420105810.jpg)
![Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Western-Blot-NB100-93388-img0003.jpg "Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93388-img0005.jpg)
![Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388] - Staining of paraffin embedded human tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Immunohistochemistry-Paraffin-NB100-93388-img0002.jpg)
![Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Flow-Cytometry-NB100-93388-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93388-img0004.jpg)
![Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Rat heart using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411404029.jpg "Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Rat heart using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Human stomach using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411364668.jpg)
![Western Blot: CaM Kinase II gamma Antibody [NBP3-37964] - Western blot analysis of various lysates using CaM Kinase II gamma Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411364615.jpg)
![Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411404069.jpg)
![Immunocytochemistry/ Immunofluorescence: CaM Kinase II gamma Antibody [NBP3-37964] - Immunofluorescence analysis of paraffin-embedded Mouse brain using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-31120251704767.jpg)
![Immunocytochemistry/ Immunofluorescence: CaM Kinase II gamma Antibody [NBP3-37964] - Immunofluorescence analysis of paraffin-embedded Rat brain using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-3112025177014.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35347] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241512133.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35347] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202414213764.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunofluorescence analysis of NIH-3T3 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202415171197.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202414163074.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241418158.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Rat lung using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241514367.jpg)
![Western Blot: TCF7L2 Antibody (1W10T6) [NBP3-15768] - Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody (NBP3-15768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TCF7L2-Antibody-1W10T6-Western-Blot-NBP3-15768-img0004.jpg "Western Blot: TCF7L2 Antibody (1W10T6) [NBP3-15768] - Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody (NBP3-15768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: TCF7L2 Antibody (1W10T6) [NBP3-15768] - Immunohistochemistry of paraffin-embedded rat testis using TCF4/TCF7L2 antibody (NBP3-15768) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TCF7L2-Antibody-1W10T6-Immunohistochemistry-Paraffin-NBP3-15768-img0002.jpg)
![Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15768_rabbit-tcf7l2-mab-1w10t6-52202517361287.jpg)
![Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15768_rabbit-tcf7l2-mab-1w10t6-52202517344294.jpg)
![Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15768_rabbit-tcf7l2-mab-1w10t6-52202517303112.jpg)
![Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15768_rabbit-tcf7l2-mab-1w10t6-5220251733510.jpg)
![Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15768_rabbit-tcf7l2-mab-1w10t6-52202517333851.jpg)
![Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-298202414304156.jpg "Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunocytochemistry analysis of Neuro-2a cells labeling Cyclin D1 with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cyclin D1 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202410484833.jpg)
![Flow Cytometry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Flow cytometric analysis of MCF7 cells labeling Cyclin D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32236, red) at 1/5,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202416453255.jpg)
![Western Blot: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Western blot analysis of Cyclin D1 on different lysates with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/5,000 dilution.Lane 1: MCF7 cell lysateLane 2: K-562 cell lysate (negative)Lane 3: A431 cell lysateLane 4: Neuro-2a cell lysateLane 5: NIH/3T3 cell lysateLane 6: C6 cell lysateLane 7: SH-SY5Y cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 34 kDaObserved band size: 35 kDaExposure time: 20 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-3920248485020.jpg)
![Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Western-Blot-NBP2-89621-img0004.jpg "Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunocytochemistry-Immunofluorescence-NBP2-89621-img0001.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human brain with rabbit mnoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0002.jpg)
![Immunoprecipitation: JNK2 Antibody (011) [NBP2-89621] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate Lane D:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MAPK9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MAPK9 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunoprecipitation-NBP2-89621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89621 antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-89621_rabbit-monoclonal-jnk2-antibody-011-23520251544322.png)
![Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse brain tissue lysate<br/> Lane 2: Jurkat cell lysate <br/> Lane 3: Rat brain tissue lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 59 kDa<br/> Observed band size: 59 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-288202412442938.png "Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse brain tissue lysate<br/> Lane 2: Jurkat cell lysate <br/> Lane 3: Rat brain tissue lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 59 kDa<br/> Observed band size: 59 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29820241426262.png)
![Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-2920241114810.png)
![Flow Cytometry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Flow cytometric analysis of MCF-7 cells labeling PPP3CB.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32848, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29202416372124.png)
![Western blot analysis on different lysates with NBP3-32848 at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32848, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa Observed band size: 59 kDa](http://images.novusbio.com/fullsize/nbp3-32848_-western-blot-639204594553835837.jpg)
![Western Blot: JNK2 Antibody [NBP1-30905] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK2-Antibody-Western-Blot-NBP1-30905-img0007.jpg "Western Blot: JNK2 Antibody [NBP1-30905] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP1-30905] - Analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30905-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP1-30905] - Paraffin-embedded N87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP1-30905-img0003.jpg)
![Western Blot: JNK2 Antibody [NBP1-30905] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/JNK2-Antibody-Western-Blot-NBP1-30905-img0004.jpg)
![Western Blot: JNK2 Antibody [NBP1-30905] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK2-Antibody-Western-Blot-NBP1-30905-img0006.jpg)
![Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0001.jpg "Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).")
![<b>Biological Strategies Validation. </b>Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0003.jpg)
![Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82009](http://images.novusbio.com/fullsize/nb100-82009_rabbit-polyclonal-jnk1-p-thr183-antibody-103202523464547.jpg)
![Western Blot: TBLR1 Antibody (3G7) [H00079718-M01] - TBL1XR1 monoclonal antibody (M01), clone 3G7 Analysis of TBL1XR1 expression in HeLa.](http://images.novusbio.com/fullsize/TBLR1-Antibody-3G7-Western-Blot-H00079718-M01-img0010.jpg "Western Blot: TBLR1 Antibody (3G7) [H00079718-M01] - TBL1XR1 monoclonal antibody (M01), clone 3G7 Analysis of TBL1XR1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: TBLR1 Antibody (3G7) [H00079718-M01] - Analysis of monoclonal antibody to TBL1XR1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TBLR1-Antibody-3G7-Immunocytochemistry-Immunofluorescence-H00079718-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: TBLR1 Antibody (3G7) [H00079718-M01] - Analysis of monoclonal antibody to TBL1XR1 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TBLR1-Antibody-3G7-Immunohistochemistry-Paraffin-H00079718-M01-img0008.jpg)
![ELISA: TBLR1 Antibody (3G7) [H00079718-M01] - Detection limit for recombinant GST tagged TBL1XR1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TBLR1-Antibody-3G7-ELISA-H00079718-M01-img0011.jpg)
![Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of PPP3CA expression in transfected 293T cell line by PPP3CA monoclonal antibody (M03), clone 2G8. Lane 1: PPP3CA transfected lysate (Predicted MW: 57.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-2G8-Western-Blot-H00005530-M03-img0006.jpg "Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of PPP3CA expression in transfected 293T cell line by PPP3CA monoclonal antibody (M03), clone 2G8. Lane 1: PPP3CA transfected lysate (Predicted MW: 57.7 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of monoclonal antibody to PPP3CA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-2G8-Immunocytochemistry-Immunofluorescence-H00005530-M03-img0003.jpg)
![Immunohistochemistry-Paraffin: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of monoclonal antibody to PPP3CA on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-2G8-Immunohistochemistry-Paraffin-H00005530-M03-img0004.jpg)
![Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of PPP3CA expression in rat brain.](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-2G8-Western-Blot-H00005530-M03-img0005.jpg)