| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08445 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87039-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61685-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42206-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13159SS | |||
![Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NBP3-14670-img0001.jpg "Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0006.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0004.jpg)
![Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0005.jpg)
![Immunoprecipitation: FoxP3 Antibody (BLR034F) [NBP3-14670] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. FOXP3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated FOXP3, was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunoprecipitation-NBP3-14670-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Immunofluorescence Analysis of PFA-fixed K562 cells using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/RXR-beta-NR2B2-Antibody-PCRP-RXRB-2B6-Immunocytochemistry-Immunofluorescence-NBP3-07586-img0005.jpg "Immunocytochemistry/Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Immunofluorescence Analysis of PFA-fixed K562 cells using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Immunohistochemistry-Paraffin: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Formalin-fixed, paraffin-embedded human lymph node stained with RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6).](http://images.novusbio.com/fullsize/RXR-beta-NR2B2-Antibody-PCRP-RXRB-2B6-Immunohistochemistry-Paraffin-NBP3-07586-img0006.jpg)
![Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Flow Cytometric Analysis of PFA-fixed U87 cells. RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/RXR-beta-NR2B2-Antibody-PCRP-RXRB-2B6-Flow-Cytometry-NBP3-07586-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Immunofluorescence Analysis of PFA-fixed HeLa cells using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/RXR-beta-NR2B2-Antibody-PCRP-RXRB-2B6-Immunocytochemistry-Immunofluorescence-NBP3-07586-img0004.jpg)
![Protein Array: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6).](http://images.novusbio.com/fullsize/RXR-beta-NR2B2-Antibody-PCRP-RXRB-2B6-Protein-Array-NBP3-07586-img0001.jpg)
![Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Flow Cytometric Analysis of PFA-fixed HeLa cells. RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/nbp3-07586_mouse-monoclonal-rxr-beta-nr2b2-antibody-pcrp-rxrb-2b6-17720241003211.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane and cytosol. Antibody stainign is shown in green.](http://images.novusbio.com/fullsize/Sirtuin-2-SIRT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87039-img0014.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane and cytosol. Antibody stainign is shown in green.")
![Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Sirtuin-2-SIRT2-Antibody-Immunohistochemistry-Paraffin-NBP1-87039-img0012.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cerebellum shows moderate cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/Sirtuin-2-SIRT2-Antibody-Immunohistochemistry-Paraffin-NBP1-87039-img0010.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/Sirtuin-2-SIRT2-Antibody-Immunohistochemistry-Paraffin-NBP1-87039-img0011.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0006.jpg "Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0004.jpg)
![Flow Cytometry: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of K562 cells using ASH2L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Flow-Cytometry-NBP2-61685-img0002.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0005.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mouse mAb against K562 (1) and F9 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0007.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of colon cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0003.jpg)
![ELISA: ASH2L Antibody (6F6B9) [NBP2-61685] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-ELISA-NBP2-61685-img0001.jpg)
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0004.jpg "Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0002.jpg)
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 . Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0003.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Western-Blot-NBP2-59213-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Immunofluorescence-NBP2-59213-img0005.jpg)
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - ChIP assays were performed using human HeLa cells, the antibody against Pol II and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter and the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Chromatin-Immunoprecipitation-NBP2-59213-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-Western-Blot-NBP2-59213-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A Antibody (C.15200004) [NBP2-59213] - Cross reactivity of the antibody directed against Pol II. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol II. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows that the antibody recognizes the unphosphorylated Pol II as well as most phosphorylated forms.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-C-15200004-ELISA-NBP2-59213-img0003.jpg)
![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0010.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0009.jpg)
![Immunoprecipitation: PAF1 Antibody [NB600-274] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 Lot 2 used for IP at 3 ug/mg lysate. Paf1 was also immunoprecipitated by rabbit anti-Paf1 Lot 1 of the same product. For blotting immunoprecipitated Paf1, NB600-274 Lot 2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunoprecipitation-NB600-274-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PAF1 Antibody [NB600-274] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 (lot NB600-274-3) used for IP at 6 ug per reaction. Paf1 was also immunoprecipitated by a previous lot of this antibody (lot NB600-274-2) and rabbit anti-Paf1 antibody NB600-273. For blotting immunoprecipitated Paf1, NB600-274 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunoprecipitation-NB600-274-img0011.jpg)
![Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP2-13159-img0002.jpg "Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-NBP2-13159-img0001.jpg)
![Simple Western: ZEB1 Antibody [NBP2-13159] - Lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Simple-Western-NBP2-13159-img0003.jpg)
![Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Western-Blot-NB100-761-img0005.jpg "Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Western-Blot-NB100-761-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761] - Detection of human ZBTB7/FBI-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody NB100-761 (lot NB100-761-3) used for IP at 3 ug per reaction. ZBTB7/FBI-1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-761-2) and rabbit anti-ZBTB7/FBI-1 antibody NB100-762. For blotting immunoprecipitated ZBTB7/FBI-1, NB100-761 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Immunoprecipitation-NB100-761-img0006.jpg)
![Western Blot: ZHX1 Antibody [NB600-244] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-ZHX1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZHX1-Antibody-Western-Blot-NB600-244-img0010.jpg "Western Blot: ZHX1 Antibody [NB600-244] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-ZHX1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- ZHX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ZHX1-Antibody-Immunohistochemistry-Paraffin-NB600-244-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ZHX1 Antibody [NB600-244] - Detection of human ZHX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZHX1 antibody NB600-244 used for IP at 3 ug per reaction. ZHX1 was also immunoprecipitated by another rabbit anti-ZHX1 antibody. For blotting immunoprecipitated ZHX1, NB600-244 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/ZHX1-Antibody-Immunoprecipitation-NB600-244-img0011.jpg)
![Western Blot: OCT4 Antibody (735) [NBP2-15051] - A. 30 ug human ESC whole cell lysate/extract 10 % SDS-PAGE dilution: 1:1000](http://images.novusbio.com/fullsize/OCT4-Antibody-735-Western-Blot-NBP2-15051-img0005.jpg "Western Blot: OCT4 Antibody (735) [NBP2-15051] - A. 30 ug human ESC whole cell lysate/extract 10 % SDS-PAGE dilution: 1:1000")
![Flow Cytometry: OCT4 Antibody (735) [NBP2-15051] - Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [735] dilution: 1:50](http://images.novusbio.com/fullsize/OCT4-Antibody-735-Flow-Cytometry-NBP2-15051-img0004.jpg)
![Western Blot: OCT4 Antibody (GT735) [NBP2-15051] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-GT735-Western-Blot-NBP2-15051-img0001.jpg)
![Western Blot: OCT4 Antibody (GT735) [NBP2-15051] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: OCT 3/4 transfected HeLa lysates 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/OCT4-Antibody-GT735-Western-Blot-NBP2-15051-img0002.jpg)
![Western Blot: OCT4 Antibody (GT735) [NBP2-15051] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OCT4-Antibody-GT735-Western-Blot-NBP2-15051-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (735) [NBP2-15051] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15051_mouse-monoclonal-oct4-antibody-735-1610202419405787.jpg)
![Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NBP2-16286-img0001.jpg "Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Frozen-NBP2-16286-img0002.jpg)
![Western Blot: EGR2 Antibody [NBP2-16286] - Sample (30 ug of whole cell lysate) A: zebrafish brain 10% SDS PAGE NBP2-16286 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16286_rabbit-polyclonal-egr2-antibody-91020242063357.jpg)
![Immunohistochemistry: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EGR2 antibody (NBP2-16286) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16286_rabbit-polyclonal-egr2-antibody-91020242014619.jpg)