| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70207 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23673SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89104-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88633-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44307SS | |||
| Azide and BSA Free | NBP2-80669 | |||
![Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Western-Blot-NBP2-02544-img0013.jpg "Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL .](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunocytochemistry-Immunofluorescence-NBP2-02544-img0014.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0012.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0003.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded colon tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0006.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded lung tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0007.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded pancreas tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0008.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded prostate tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0009.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0010.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded bladder tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0011.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0018.jpg "Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunocytochemistry-Immunofluorescence-NBP2-00723-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0016.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0012.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0015.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0011.jpg)
![Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Simple-Western-NBP2-00723-img0010.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NB100-81909-img0010.jpg "Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - ICC/IF showing estrogen receptor alpha (green) in human cervical stromal cells. Nuclei are stained with DAPI (blue). Antibody at 1:200 in 3% BSA. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81909-img0009.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-81909 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NB100-81909-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of rat-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241523500.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-2342024155515.jpg)
![Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of Hela cells.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414521010.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of lysates from 293 cells, using NB100-81909. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414462211.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Human-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241521260.jpg)
![Western Blot: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of Pokemon in MCF7 cell lysate. Lane 1 shows pre-immune sera and lane 2 shows Pokemon antibody tested on MCF7 at 1:1000 dilution.](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Western-Blot-NBP2-23673-img0005.jpg "Western Blot: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of Pokemon in MCF7 cell lysate. Lane 1 shows pre-immune sera and lane 2 shows Pokemon antibody tested on MCF7 at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of human skin using Pokemon antibody at 1:100.](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Immunohistochemistry-Paraffin-NBP2-23673-img0004.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89104-img0012.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human tonsil shows moderate to strong nuclear positivity in a subset lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human rectum shows moderate to strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0009.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human salivary gland shows moderate to strong nuclear positivity in ductal cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RUNX2/CBFA1 Antibody [NBP1-89104] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Knockdown-Validated-NBP1-89104-img0014.jpg)
![Immunohistochemistry-Frozen: TCF4 Antibody [NBP1-88633] - Frozen mouse heart sections. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TCF4-Antibody-Immunohistochemistry-NBP1-88633-img0005.jpg "Immunohistochemistry-Frozen: TCF4 Antibody [NBP1-88633] - Frozen mouse heart sections. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/antibody/nbp1-88633_rabbit-polyclonal-tcf4-antibody-immunohistochemistry-paraffin-271020239341..jpg)
![Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp1-88633_rabbit-polyclonal-tcf4-antibody-immunohistochemistry-paraffin-271020239446..jpg)
![Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88633_rabbit-polyclonal-tcf4-antibody-immunohistochemistry-paraffin-271020239556..jpg)
![Immunohistochemistry-Paraffin: TCF4 Antibody [NBP1-88633] -Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-88633_rabbit-polyclonal-tcf4-antibody-immunohistochemistry-paraffin-271020239656..jpg)
![Western Blot: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:5000 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB200-342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Carm1-Antibody-Knockdown-Validated-NB200-342-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:5000 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB200-342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB200-342-img0015.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. A300-421A lot 4) used at 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB200-342-img0016.jpg)
![Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (10 ug) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB200-342-img0014.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB200-342-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Carm1 Antibody [NB200-342] - Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody NB200-342 (lot NB200-342-4) used for IP at 6 ug per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (lot NB200-342-3) and another rabbit anti-CARM1 antibody. For blotting immunoprecipitated CARM1, NB200-342 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunoprecipitation-NB200-342-img0012.jpg)
![Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NB600-233-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-233-img0009.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 asdetected using anti-Sp1.](http://images.novusbio.com/fullsize/SP1-Antibody-Chromatin-Immunoprecipitation-NB600-233-img0014.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-233-img0011.jpg)
![Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used at 0.04 mcg/ml for WB.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunoprecipitation-NB600-233-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody NB600-233 (lot NB600-233-5) used for IP at 6 ug per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (NB600-233-4) and another rabbit anti-SP1 antibody. For blotting immunoprecipitated SP1, NB600-233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunoprecipitation-NB600-233-img0015.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Notch1 affects CREB phosphorylation and transcription. Immunoblot on whole hippocampal lysates shows a reduction in CREB phosphorylation in N1cKO as compared to WT (n = 3 independent experiments). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/Article/10.3389/fncel.2015.00447/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0026.jpg "Western Blot: CREB Antibody (LB9) [NB100-74393] - Notch1 affects CREB phosphorylation and transcription. Immunoblot on whole hippocampal lysates shows a reduction in CREB phosphorylation in N1cKO as compared to WT (n = 3 independent experiments). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/Article/10.3389/fncel.2015.00447/abstract) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0019.jpg)
![Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Staining of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:400 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0025.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0021.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Analysis of HepG2 cells](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0022.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Cancer biopsies of deparaffinized human bladder and rectal tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0018.jpg)
![Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0024.jpg)
![Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB was performed on untreated 293T cells. Antigen: antbody complex was formed by binding 500ug whole cell lysate with 2ug of mouse monoclonal antibody recognizing CREB overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:1000 overnight rotating at 4C. Membranes were then washed in TBST and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunoprecipitation-NB100-74393-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunocytochemistry-Immunofluorescence-NBP2-44307-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern in the cytoplasm of cancer cells. Some cells depicted nuclear positivity also while the stroma was largely negative for CHREBP expression.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0003.jpg)
![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern mainly in the cytoplasm (but in some nuclei also) of cuboidal epithelial cells of various ducts and tubules.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0002.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0013.jpg "Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunocytochemistry-Immunofluorescence-H00000860-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunohistochemistry-Paraffin-H00000860-M01-img0010.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in SJCRH30 ( Cat # L027V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0001.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0003.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - RUNX2 monoclonal antibody (M01), clone 1D8. Analysis of RUNX2 expression in Jurkat.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0012.jpg)
![Sandwich ELISA: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Sandwich-ELISA-H00000860-M01-img0011.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)