| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70399 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74356 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71688 | |||
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-91020242041215.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-91020242075965.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-910202420115040.jpg)
![GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-immunohistochemistry-paraffin-141120251842513.jpg)
![GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-immunohistochemistry-paraffin-141120251825205.jpg)
![GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at mitochondria in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-immunohistochemistry-paraffin-1411202518352221.jpg)
![Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NFYB-Antibody-Immunohistochemistry-Paraffin-NBP2-19535-img0005.jpg "Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Mouse intestine. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NFYB-Antibody-Immunohistochemistry-Paraffin-NBP2-19535-img0004.jpg)
![Western Blot: NFYB Antibody [NBP2-19535] - Various tissue extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-19535_rabbit-polyclonal-nfyb-antibody-91020242063327.jpg)
![Immunohistochemistry: NFYB Antibody [NBP2-19535] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (NBP2-19535) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-19535_rabbit-polyclonal-nfyb-antibody-9102024202196.jpg)
![Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19535_rabbit-polyclonal-nfyb-antibody-91020242041218.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16054-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.")
![Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054] - Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 ug of Cyclin D1 antibody [N1C3]. Western blot analysis was performed using Cyclin D1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunoprecipitation-NBP2-16054-img0006.jpg)
![ELISA: Cyclin D1 Antibody [NBP2-16054] - Detection of recombinant full-length Cyclin D1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-ELISA-NBP2-16054-img0014.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-910202420122096.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-16054] - Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Cyclin D1 stained by Cyclin D1 antibody (NBP2-16054) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-910202420104464.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-91020242063359.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-91020242075916.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody [NBP2-16054] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-1610202419414560.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16054_rabbit-polyclonal-cyclin-d1-antibody-western-blot-26112025182002.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0012.jpg "Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP1-31324] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31324-img0008.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324] - Paraffin-embedded gastric tissue, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31324-img0003.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0004.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP1-31324] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3].](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0007.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0010.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31324 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-910202420134090.jpg)
![Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Enolase 1 antibody [N3C3] (NBP1-31324) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-91020242041252.jpg)
![Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Enolase 1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Enolase 1 antibody [N3C3] (NBP1-31324) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-910202420134054.jpg)
![Western Blot: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UNG-Antibody-Knockdown-Validated-NBP1-49985-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody which recognizes an upstream epitope. For blotting immunoprecipitated UNG was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP1-49985-img0002.jpg)
![Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0003.jpg "Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.")
![Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0002.jpg)
![Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Western-Blot-NBP2-01245-img0003.jpg "Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.")
![Immunocytochemistry/Immunofluorescence: CDKN3 Antibody (2E11) [NBP2-01245] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunocytochemistry-Immunofluorescence-NBP2-01245-img0009.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human prostate tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0008.jpg)
![Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Flow-Cytometry-NBP2-01245-img0002.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0004.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0005.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0006.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human Kidney tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0007.jpg)
![Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - Analysis of Jurkat cells, using anti-CDKN3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Flow-Cytometry-NBP2-01245-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0007.jpg "Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunocytochemistry-Immunofluorescence-NBP1-47754-img0006.jpg)
![Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-OTI1F10-Immunohistochemistry-NBP1-47754-img0008.jpg)
![Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Flow-Cytometry-NBP1-47754-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0002.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0004.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0005.jpg)
![Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Western-Blot-NBP1-48002-img0015.jpg "Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunocytochemistry-Immunofluorescence-NBP1-48002-img0013.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0012.jpg)
![Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Western-Blot-NBP1-48002-img0001.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0002.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0003.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0004.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0005.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0006.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0007.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0008.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0009.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0010.jpg)
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0011.jpg)
![Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunoprecipitation-NBP1-48002-img0016.jpg)
![SSB Antibody (OTI2C8)[NBP1-48002]Simple Western� analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp1-48002_mouse-ssb-mab-oti2c8-simple-western-131120251236912.jpg%253fstore=america&image-type=image)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0020.jpg "Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) [NBP1-47695] - Chk2 Antibody (5C4) [NBP1-47695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunocytochemistry-Immunofluorescence-NBP1-47695-img0013.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0019.jpg)
![Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Flow-Cytometry-NBP1-47695-img0012.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0011.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0014.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0015.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0016.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0017.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0018.jpg)
![Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Western-Blot-NBP2-22481-img0001.jpg "Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 in NCCIT and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a LIN28 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the cytoplasm of human seminoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0015.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0013.jpg)
![Chromatin Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay of LIN28 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2-3 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the rat Egr-1 locus is shown; oxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Chromatin-Immunoprecipitation-ChIP-NBP2-22481-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the nucleus and cytoplasm of mouse testis tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0014.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0012.jpg)
![Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 was performed. Antigen-antibody complexes were formed by incubating 700ug of lysate with 5 ug of an LIN28 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Agarose was loaded as a positive control for detection. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a LIN28 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunoprecipitation-NBP2-22481-img0002.jpg)