| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52424-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48305 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19344SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47917-0.1mg | |||
| IHC-Prediluted | NBP2-48306 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50048SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34413-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47698-0.1mg | |||
| IHC-Prediluted | NBP2-48132 | |||
![Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-BL-178-12C7-Western-Blot-NBP3-14668-img0013.jpg "Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody [BL-178-12C7](A700-012). Secondary: HRP-conjugated goat anti-rabbitIgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0006.jpg)
![Immunohistochemistry: CD45 Antibody [NBP3-14668] - Sample: FFPE section of human coloncarcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™.Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0007.jpg)
![Flow Cytometry: CD45 Antibody [NBP3-14668] - Sample: Jurkat cells were fixed in 4% formaldehyde andpermeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45recombinant monoclonal antibody (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP3-14668-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0004.jpg)
![Immunoprecipitation: CD45 Antibody [NBP3-14668] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from Jurkat cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-CD45recombinant monoclonal antibody used for IP at 20 ul/mg lysate. CD45 wasalso immunoprecipitated by a previous lot of this antibodyand rabbit anti-CD45 antibody. For blotting immunoprecipitated CD45,was used at 1:1000. Chemiluminescence withan exposure time of 250 milliseconds.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunoprecipitation-NBP3-14668-img0002.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0009.jpg "Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Immunocytochemistry-Immunofluorescence-NBP2-52424-img0006.jpg)
![Flow Cytometry: BMP-4 Antibody (3C11C7) [NBP2-52424] - Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Flow-Cytometry-NBP2-52424-img0010.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0004.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against human BMP4 recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Immunocytochemistry-Immunofluorescence-NBP2-52424-img0005.jpg)
![ELISA: BMP-4 Antibody (3C11C7) [NBP2-52424] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-ELISA-NBP2-52424-img0007.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0007.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunocytochemistry-Immunofluorescence-NBP2-22204-img0002.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Flow-Cytometry-NBP2-22204-img0001.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0006.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0003.jpg)
![ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-ELISA-NBP2-22204-img0005.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Comparison on osteogenic-related proteins (OCN, BMP2, COL1a1, DDk1, ALP) for osteogenic micro-tissue and digested cells by Western blot. MT, osteogenic micro-tissue; DC, digested cells Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1064-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Western-Blot-NB600-540-img0009.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Comparison on osteogenic-related proteins (OCN, BMP2, COL1a1, DDk1, ALP) for osteogenic micro-tissue and digested cells by Western blot. MT, osteogenic micro-tissue; DC, digested cells Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1064-1) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540] - Confocal fluorescent analysis of A431 using anti-TRA-2-49 Mouse monoclonal antibody (Red). Actin filaments have been labeled with AlexaFluor(R) 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive cytosol staining.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-540-img0011.jpg)
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Flow-Cytometry-NB600-540-img0008.jpg)
![Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-VIPL2-BSA-Free-Flow-Cytometry-NBP1-97563-img0007.jpg "Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.")
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0001.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0002.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0003.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0004.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PerCP.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0005.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) FITC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0006.jpg)
![Western Blot: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Western Blot Analysis of PC-3 cell lysate using Moesin antibody (MSN/492).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Western-Blot-NBP2-47916-img0007.jpg "Western Blot: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Western Blot Analysis of PC-3 cell lysate using Moesin antibody (MSN/492).")
![Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. Moesin Antibody (MSN/492) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47916-img0001.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47916-img0006.jpg)
![Flow Cytometry: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Flow Cytometric Analysis of K562 cells. Moesin Antibody (MSN/492) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47916-img0003.jpg)
![Western Blot: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Western Blot Analysis of human Jurkat cell lysate using Moesin Antibody (MSN/492).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Western-Blot-NBP2-47916-img0002.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47916-img0004.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47916-img0005.jpg)
![Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Western-Blot-NBP1-19344-img0005.jpg "Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.")
![Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-NBP1-19344-img0004.jpg)
![Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Simple-Western-NBP1-19344-img0006.jpg)
![Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44580) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Knockout-Validated-NBP2-44580-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44580) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Immunocytochemistry-Immunofluorescence-NBP2-44580-img0006.jpg)
![Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western Blot Analysis of PC3 cell lysate. Moesin antibody (MSN/493).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Western-Blot-NBP2-44580-img0010.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/493)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Immunohistochemistry-Paraffin-NBP2-44580-img0003.jpg)
![Flow Cytometry: Moesin Antibody (MSN/493) [NBP2-44580] - Flow Cytometric Analysis of paraformaldehyde-fixed K562 cells. Moesin Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Flow-Cytometry-NBP2-44580-img0005.jpg)
![Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western Blot Analysis of human Jurkat cell lysate. Moesin Antibody (MSN/493).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Western-Blot-NBP2-44580-img0007.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/493)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Immunohistochemistry-Paraffin-NBP2-44580-img0001.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/493)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Immunohistochemistry-Paraffin-NBP2-44580-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with anti-Moesin (NBP2-44580) antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining), and far-red (KO) channels was performed. Representative grayscale images of the red channel are shown, and WT and KO cells are outlined with a yellow and magenta dashed line, respectively. A schematic representation of the mosaic strategy used is shown on the right panel. Antibody dilution used: NBP2-44579 at 1/200. Bar = 10 um. Images and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-493-Knockout-Validated-NBP2-44580-img0009.jpg)
![Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Western-Blot-NBP2-50048-img0002.jpg "Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-50048-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of rat brain cerebellum section stained with mouse mAb to calbindin, NBP2-50048, dilution 1:1,000, in green, and costained with rabbit pAb to calretinin, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-50048-img0004.jpg)
![Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Western-Blot-NBP2-34365-img0004.jpg "Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM507) [NBP2-34365] - Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Immunohistochemistry-Paraffin-NBP2-34365-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunocytochemistry-Immunofluorescence-NBP2-44463-img0008.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Rat Lung stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Intracellular-NBP2-44463-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Angiosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Colon Carcinoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Leiomyosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0003.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HepG2 cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Cytometry-NBP2-44463-img0006.jpg)
![Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB110-93480-img0001.jpg "Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.")
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Showing specific labeling of GFAP (red) and vimentin (green) cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000, green), colabeled with anti-MeCP2 (1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000,green), colabeled with anti-calbindin (1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0005.jpg)