| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70129 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74318 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52424-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP1-96988 | |||
| Rhodamine | NBP1-96881 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47862-0.1mg | |||
| IHC-Prediluted | NBP2-48320 | |||
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0007.jpg "Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunocytochemistry-Immunofluorescence-NBP1-47754-img0006.jpg)
![Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-OTI1F10-Immunohistochemistry-NBP1-47754-img0008.jpg)
![Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Flow-Cytometry-NBP1-47754-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0002.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0004.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0005.jpg)
![Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] Analysis of extracts (35ug) from 9 different cell lines by using anti-AK3L1 monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Western-Blot-NBP2-00827-img0012.jpg "Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] Analysis of extracts (35ug) from 9 different cell lines by using anti-AK3L1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK3L1.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunocytochemistry-Immunofluorescence-NBP2-00827-img0011.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human liver tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0010.jpg)
![Flow Cytometry: AK3L1 Antibody (3B1) [NBP2-00827] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK3L1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Flow-Cytometry-NBP2-00827-img0001.jpg)
![Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3L1.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Western-Blot-NBP2-00827-img0002.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0003.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0004.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0005.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0006.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human colon tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0007.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human endometrium tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0008.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human Kidney tissue using anti-AK3L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK3L1-Antibody-3B1-Immunohistochemistry-Paraffin-NBP2-00827-img0009.jpg)
![Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Spermine synthase.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Western-Blot-NBP2-00900-img0021.jpg "Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Spermine synthase.")
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human tonsil using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0020.jpg)
![Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Spermine synthase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Flow-Cytometry-NBP2-00900-img0013.jpg)
![Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Spermine synthase monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Western-Blot-NBP2-00900-img0011.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0014.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0015.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0016.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0017.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human Kidney tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0018.jpg)
![Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human lymphoma tissue using anti-Spermine synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Immunohistochemistry-Paraffin-NBP2-00900-img0019.jpg)
![Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Analysis of Hela cells, using anti-Spermine synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Spermine-synthase-Antibody-OTI3C9-Flow-Cytometry-NBP2-00900-img0012.jpg)
![Western Blot: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of extracts of various cell lines, using CD9 antibody (NBP3-15593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/CD9-Antibody-10N2L6-Western-Blot-NBP3-15593-img0002.jpg "Western Blot: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of extracts of various cell lines, using CD9 antibody (NBP3-15593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593] - Human lung cancer using CD9 Rabbit mAb (NBP3-15593) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD9-Antibody-10N2L6-Immunohistochemistry-Paraffin-NBP3-15593-img0001.jpg)
![Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of 300ug extracts of THP-1 cells using 3ug CD9 antibody (NBP3-15593). Western blot was performed from the immunoprecipitate using CD9 antibody (NBP3-15593) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/CD9-Antibody-10N2L6-Immunoprecipitation-NBP3-15593-img0003.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0009.jpg "Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Immunocytochemistry-Immunofluorescence-NBP2-52424-img0006.jpg)
![Flow Cytometry: BMP-4 Antibody (3C11C7) [NBP2-52424] - Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Flow-Cytometry-NBP2-52424-img0010.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0004.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against human BMP4 recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Immunocytochemistry-Immunofluorescence-NBP2-52424-img0005.jpg)
![ELISA: BMP-4 Antibody (3C11C7) [NBP2-52424] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-ELISA-NBP2-52424-img0007.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NB100-93304-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-310202416284928.jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-31020241628498.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0018.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33498-img0009.jpg)
![Immunohistochemistry-Paraffin: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Mouse cervix. PSME3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunohistochemistry-Paraffin-NBP1-33498-img0016.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0013.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: PC-12 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0014.jpg)
![Immunohistochemistry-Paraffin: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - CA922 xenograft, using PSME3 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunohistochemistry-Paraffin-NBP1-33498-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: MTHFD2 Antibody [NBP1-33200] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTHFD2 stained by MTHFD2 antibody [N1C3] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/MTHFD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33200-img0016.jpg "Immunocytochemistry/Immunofluorescence: MTHFD2 Antibody [NBP1-33200] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTHFD2 stained by MTHFD2 antibody [N1C3] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human MCF7 xenograft. MTHFD2 antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MTHFD2-Antibody-Immunohistochemistry-Paraffin-NBP1-33200-img0011.jpg)
![Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MTHFD2-Antibody-Immunohistochemistry-Paraffin-NBP1-33200-img0006.jpg)
![Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human breast carcinoma diluted at 1:500.](http://images.novusbio.com/fullsize/MTHFD2-Antibody-Immunohistochemistry-Paraffin-NBP1-33200-img0008.jpg)
![Immunoprecipitation: MTHFD2 Antibody [NBP1-33200] - Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 ug of MTHFD2 antibody [N1C3] Western blot analysis was performed using MTHFD2 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MTHFD2-Antibody-Immunoprecipitation-NBP1-33200-img0010.jpg)
![Western Blot: MTHFD2 Antibody [NBP1-33200] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (NBP1-33200) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33200_rabbit-polyclonal-mthfd2-antibody-91020242012202.jpg)
![Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.MTHFD2 stained by MTHFD2 antibody [N1C3] (NBP1-33200) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33200_rabbit-polyclonal-mthfd2-antibody-9102024205650.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MTHFD2 Antibody [NBP1-33200] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33200_rabbit-polyclonal-mthfd2-antibody-1610202419405764.jpg)
![SDS-Page: Albumin Native Protein [NBP1-97049] - SDS PAGE of Human Albumin. Lane 1: Non-Reduced Human Albumin. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Human Albumin. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 63 kDa/Observed at 55 kDa; Reduced at 63 kDa. Non-reduced migrates farther on gel due to native albumin's capacity to bind ions, increasing its charge and, therefore, ability to migrate farther down gel.](http://images.novusbio.com/fullsize/Albumin-Native-Protein-SDS-Page-NBP1-97049-img0002.jpg "SDS-Page: Albumin Native Protein [NBP1-97049] - SDS PAGE of Human Albumin. Lane 1: Non-Reduced Human Albumin. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Human Albumin. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 63 kDa/Observed at 55 kDa; Reduced at 63 kDa. Non-reduced migrates farther on gel due to native albumin's capacity to bind ions, increasing its charge and, therefore, ability to migrate farther down gel.")
![Dot Blot: Albumin Native Protein [NBP1-97049] - Human Albumin Rhodamine Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 HR at RT.. Using the Rhodamine format of this antibody.](http://images.novusbio.com/fullsize/Albumin-Native-Protein-Dot-Blot-NBP1-97049-img0004.jpg)
![Dot Blot: Albumin Native Protein [NBP1-97049] - Human Albumin Rhodamine Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 HR at RT.. Using the Rhodamine format of this antibody.](http://images.novusbio.com/fullsize/Albumin-Native-Protein-Dot-Blot-NBP1-97049-img0005.jpg)
![SDS-Page: Albumin Native Protein [NBP1-97049] - SDS-PAGE results of Human Albumin. Lane 1: reduced human albumin. Lane 2: opal prestained molecular weight ladder. Lane 3: non-reduced human albumin. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.](http://images.novusbio.com/fullsize/Albumin Native Protein-SDS-Page-NBP1-97049-img0006.jpg)
![Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16382-img0005.jpg)
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0010.jpg)
![Western Blot: ETFA Antibody [NBP2-16382] - 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0001.jpg)
![Western Blot: ETFA Antibody [NBP2-16382] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0002.jpg)
![Western Blot: ETFA Antibody [NBP2-16382] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0008.jpg)
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0004.jpg)
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0007.jpg)
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0009.jpg)
![Immunoprecipitation: ETFA Antibody [NBP2-16382] - Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 ug of ETFA antibody. Western blot analysis was performed using ETFA antibody.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunoprecipitation-NBP2-16382-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44613-img0009.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0010.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Flow-Cytometry-NBP2-44613-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44613-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Uterus stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0006.jpg)