| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21480-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56159SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0340A488-100 | |||
| Alexa Fluor 546 | DDX0340A546-100 | |||
| Alexa Fluor 647 | DDX0340A647 | |||
![Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486] - Analysis of TCR Beta F1 in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/TCR-beta-Antibody-8A3-Flow-Cytometry-NBP2-22486-img0001.jpg "Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486] - Analysis of TCR Beta F1 in Jurkat cells (green) compared to an isotype control (blue).")
![Western Blot: PKC gamma Antibody [NBP1-58916] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Western-Blot-NBP1-58916-img0004.jpg "Western Blot: PKC gamma Antibody [NBP1-58916] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.")
![Chromatin Immunoprecipitation: PKC gamma Antibody [NBP1-58916] - Quiescent human colon carcinoma cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-58916-img0005.jpg)
![Western Blot: PKC gamma Antibody [NBP1-58916] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Western-Blot-NBP1-58916-img0002.jpg)
![ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-244202310354.jpg "ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-2442023103658.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517344223.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517311071.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-5220251733559.jpg)
![Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Western-Blot-NB500-141-img0003.jpg "Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Immunohistochemistry-NB500-141-img0004.jpg)
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-168202375610..jpg "Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.")
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-16820237586..jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-168202375944.jpg)
![<b>Biological Strategies Validation. </b>Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238120.jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238442..jpg)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-136202422345072.png)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-23520251628115.png)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0006.jpg "Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Analysis of anti-TNIK antibody with kidney at 2 ug/ml.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0002.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Analysis of anti-TNIK antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0003.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0004.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0005.jpg)
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0001.jpg "Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-26112025182908.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251843456.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251850424.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-15553-img0007.jpg "Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunohistochemistry-Paraffin-NBP2-15553-img0003.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-15553-img0002.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] (NBP2-15553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15553_rabbit-polyclonal-bckdk-antibody-910202420104442.jpg)
![Western Blot: PERK Antibody [NBP1-78017] - PERK in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipitated with 15ul anti-PERK, followed by western blotIgG with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2, 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.](http://images.novusbio.com/fullsize/PERK Antibody-Western Blot-NBP1-78017-img0011.jpg "Western Blot: PERK Antibody [NBP1-78017] - PERK in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipitated with 15ul anti-PERK, followed by western blotIgG with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2, 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: PERK Antibody [NBP1-78017] - Analysis of mouse mammary gland samples from lactating mice (L10) with anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-NBP1-78017-img0010.jpg)
![Western Blot: IRAK4 Antibody (6F8) [DDX0340P-100] - Western Blot with 6F8 (Innateplateform, CLARA, Lyon)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Western-Blot-DDX0340P-100-img0011.jpg "Western Blot: IRAK4 Antibody (6F8) [DDX0340P-100] - Western Blot with 6F8 (Innateplateform, CLARA, Lyon)")
![Immunohistochemistry: IRAK4 Antibody (6F8) [DDX0340P-100] - IHC staining of human FFPE tonsil section(Vermi William courtesy)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Immunohistochemistry-DDX0340P-100-img0009.jpg)
![Flow Cytometry: IRAK4 Antibody (6F8) [DDX0340P-100] - Intracellular FACS Staining of canine cell line with 6F8 (Bonnefont C, personal comm, 2011).](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Flow-Cytometry-DDX0340P-100-img0003.jpg)
![Immunohistochemistry: IRAK4 Antibody (6F8) [DDX0340P-100] - Analysis of human tonsil with 6F8 (DDX0340)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Immunohistochemistry-DDX0340P-100-img0008.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NB100-293-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")