| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37964-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37292-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61707-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89621-50ul | |||
![Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Rat heart using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411404029.jpg "Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Rat heart using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Human stomach using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411364668.jpg)
![Western Blot: CaM Kinase II gamma Antibody [NBP3-37964] - Western blot analysis of various lysates using CaM Kinase II gamma Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411364615.jpg)
![Immunohistochemistry: CaM Kinase II gamma Antibody [NBP3-37964] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-1012202411404069.jpg)
![Immunocytochemistry/ Immunofluorescence: CaM Kinase II gamma Antibody [NBP3-37964] - Immunofluorescence analysis of paraffin-embedded Mouse brain using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-31120251704767.jpg)
![Immunocytochemistry/ Immunofluorescence: CaM Kinase II gamma Antibody [NBP3-37964] - Immunofluorescence analysis of paraffin-embedded Rat brain using CaM Kinase II gamma Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37964_rabbit-cam-kinase-ii-gamma-pab-3112025177014.jpg)
![Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-5220251734544.jpg "Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.")
![Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517315598.jpg)
![Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human skin using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517344236.jpg)
![Immunocytochemistry/ Immunofluorescence: CD44 Antibody (9W6E7) [CD44] - Confocal imaging of HeLa cells using CD44 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517352722.jpg)
![Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and HeLa cells were surface-stained with CD44 Rabbit mAb or ABflo 647 Rabbit IgG isotype control , followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-5220251735270.jpg)
![Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo 647 Rabbit IgG isotype control or CD44 Rabbit mAb .](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517304969.jpg)
![Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9B9-Western-Blot-NBP2-37292-img0013.jpg "Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of HepG2 cells using CSF1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9B9-Immunocytochemistry-Immunofluorescence-NBP2-37292-img0009.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of esophageal tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9B9-Immunohistochemistry-NBP2-37292-img0011.jpg)
![Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against human CSF1R (AA: 344-497) recombinant protein. (Expected MW is 43.3 kDa)](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9B9-Western-Blot-NBP2-37292-img0012.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of pancreas tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9B9-Immunohistochemistry-NBP2-37292-img0010.jpg)
![ELISA: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9B9-ELISA-NBP2-37292-img0008.jpg)
![Western Blot: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)](http://images.novusbio.com/fullsize/MKK7-MEK7-Antibody-4E5-Western-Blot-NBP2-37542-img0002.jpg "Western Blot: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)")
![Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunofluorescence analysis of Hela cells using MAP2K7 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/MKK7-MEK7-Antibody-4E5-Immunocytochemistry-Immunofluorescence-NBP2-37542-img0003.jpg)
![Immunohistochemistry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MKK7-MEK7-Antibody-4E5-Immunohistochemistry-NBP2-37542-img0006.jpg)
![Flow Cytometry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Flow cytometric analysis of Hela cells using MAP2K7 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MKK7-MEK7-Antibody-4E5-Flow-Cytometry-NBP2-37542-img0004.jpg)
![Immunohistochemistry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MAP2K7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MKK7-MEK7-Antibody-4E5-Immunohistochemistry-NBP2-37542-img0005.jpg)
![ELISA: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MKK7-MEK7-Antibody-4E5-ELISA-NBP2-37542-img0001.jpg)
![Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mAb against HEK293 (1) and BCR (AA: 139-280)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BCR-Antibody-1E11G12-Western-Blot-NBP2-61707-img0004.jpg "Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mAb against HEK293 (1) and BCR (AA: 139-280)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: BCR Antibody (1E11G12) [NBP2-61707] - Analysis of K562 cells using BCR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BCR-Antibody-1E11G12-Flow-Cytometry-NBP2-61707-img0002.jpg)
![Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mAb against human BCR (AA: 139-280) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/BCR-Antibody-1E11G12-Western-Blot-NBP2-61707-img0003.jpg)
![Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mouse mAb against Jurkat (1), Hela (2), and Ramos (3) cell lysate.](http://images.novusbio.com/fullsize/BCR-Antibody-1E11G12-Western-Blot-NBP2-61707-img0005.jpg)
![ELISA: BCR Antibody (1E11G12) [NBP2-61707] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BCR-Antibody-1E11G12-ELISA-NBP2-61707-img0001.jpg)
![Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-644-img0002.jpg "Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.")
![Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NB100-644-img0003.jpg)
![Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Western-Blot-NBP2-89621-img0004.jpg "Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunocytochemistry-Immunofluorescence-NBP2-89621-img0001.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human brain with rabbit mnoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0002.jpg)
![Immunoprecipitation: JNK2 Antibody (011) [NBP2-89621] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate Lane D:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MAPK9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MAPK9 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunoprecipitation-NBP2-89621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89621 antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-89621_rabbit-monoclonal-jnk2-antibody-011-23520251544322.png)
![Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-16A1-Flow-Cell-Surface-NBP1-44581-img0005.jpg)
![Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-16A1-Flow-Cytometry-NBP1-44581-img0001.jpg)
![Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-16A1-Flow-Cytometry-NBP1-44581-img0002.jpg)
![Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) FITC.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-16A1-Flow-Cell-Surface-NBP1-44581-img0003.jpg)
![Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-16A1-Flow-Cell-Surface-NBP1-44581-img0004.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![<b>Biological Strategies Validation. </b>ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67373-img0006.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0004.jpg)