| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-9287-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74022 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54571-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82243-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30091SS | |||
| Azide and BSA Free | NBP2-80771 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49546SS | |||
![Flow Cytometry: Cytokeratin 8 Antibody (AE3) [NB120-9287] - An intracellular stain was performed on A431 cells with Cytokeratin 8 Antibody [AE3] NB120-9287AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-AE3-Flow-Cytometry-NB120-9287-img0001.jpg "Flow Cytometry: Cytokeratin 8 Antibody (AE3) [NB120-9287] - An intracellular stain was performed on A431 cells with Cytokeratin 8 Antibody [AE3] NB120-9287AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Western-Blot-NB100-81896-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Immunohistochemistry-Paraffin-NB100-81896-img0011.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-23420241523258.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-103202523464519.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-1132025155593.jpg)
![Western Blot: SCL/Tal1 Antibody (5H1) [NBP2-01488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tal1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tal1.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Western-Blot-NBP2-01488-img0014.jpg "Western Blot: SCL/Tal1 Antibody (5H1) [NBP2-01488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tal1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tal1.")
![Immunocytochemistry/Immunofluorescence: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tal1.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Immunocytochemistry-Immunofluorescence-NBP2-01488-img0010.jpg)
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of paraffin-embedded Human prostate tissue using anti-Tal1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Immunohistochemistry-Paraffin-NBP2-01488-img0013.jpg)
![Flow Cytometry: SCL/Tal1 Antibody (5H1) [NBP2-01488] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Tal1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Flow-Cytometry-NBP2-01488-img0009.jpg)
![Western Blot: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Analysis of extracts (10ug) from 2 different cell lines by using anti-TAL1 monoclonal antibody at 1:200.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Western-Blot-NBP2-01488-img0008.jpg)
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Tal1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Immunohistochemistry-Paraffin-NBP2-01488-img0011.jpg)
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of paraffin-embedded Human colon tissue using anti-Tal1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-5H1-Immunohistochemistry-Paraffin-NBP2-01488-img0012.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunocytochemistry-Immunofluorescence-NBP2-67353-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-elF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0013.jpg)
![Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Flow-Cytometry-NBP2-67353-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0012.jpg)
![Western blot analysis on different lysates with NBP2-67353 at 1/2,000 dilution. Lane 1: HeLa whole cell lysate (15 µg/Lane) Lane 2: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate (15 µg/Lane) Lane 3: NIH/3T3 whole cell lysate (15 µg/Lane) Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (15 µg/Lane) Lane 5: C6 whole cell lysate (20 µg/Lane) Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: Lane 1-4: 2 minutes; Lane 5-6: 23 seconds； 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67353 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67353_-western-blot-639204586669420877.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western blot analysis on different lysates with NBP2-67471 at 1/2,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 5: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes, then treated with lambda pp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67471 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67471_-western-blot-639204586672256172.jpg)
![Western blot analysis on different lysates with NBP2-67471 at 1/500 dilution. Lane 1: A549 whole cell lysate Lane 2: A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67471 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67471_-western-blot-639204597490896795.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-fixed, paraffin-embedded human Tongue Carcinoma stained with CD44 Antibody (CD44v9/1459) - variant 9.](http://images.novusbio.com/fullsize/CD44-Antibody-CD44v9-1459-variant-9-Immunohistochemistry-Paraffin-NBP2-53204-img0004.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-fixed, paraffin-embedded human Tongue Carcinoma stained with CD44 Antibody (CD44v9/1459) - variant 9.")
![Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Cervical Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)](http://images.novusbio.com/fullsize/CD44-Antibody-CD44v9-1459-variant-9-Immunohistochemistry-Paraffin-NBP2-53204-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Prostate Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)](http://images.novusbio.com/fullsize/CD44-Antibody-CD44v9-1459-variant-9-Immunohistochemistry-Paraffin-NBP2-53204-img0002.jpg)
![Western Blot: Tau [p Ser396] Antibody [NB100-82243] - Extracts from mouse brain tissue using Tau(p Ser396) Antibody and same antibody preincubated with blocking peptide](http://images.novusbio.com/fullsize/Tau-[p-Ser396]-Antibody-Western-Blot-NB100-82243-img0003.jpg "Western Blot: Tau [p Ser396] Antibody [NB100-82243] - Extracts from mouse brain tissue using Tau(p Ser396) Antibody and same antibody preincubated with blocking peptide")
![Immunohistochemistry: Tau [p Ser396] Antibody [NB100-82243] - Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using NB100-82243.](http://images.novusbio.com/fullsize/nb100-82243_rabbit-polyclonal-tau-p-ser396-antibody-103202523321010.jpg)
![p70-S6-Kinase-S6K-[p-Thr389-412]-Antibody-Western-Blot-NB600-1049-img0005.jpg](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389-412]-Antibody-Western-Blot-NB600-1049-img0005.jpg "<b>Biological Strategies Validation. </b>p70-S6-Kinase-S6K-[p-Thr389-412]-Antibody-Western-Blot-NB600-1049-img0005.jpg")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1049-img0002.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Human liver cancer tissue sections](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Immunohistochemistry-NB600-1049-img0003.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of phospho-p70 S6 Kinase in human breast cancer cell lines (MDA-MB-231 and MDA-MB-435) using p70 S6 Kinase/S6K [p Thr390, p Thr412] Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Western-Blot-NB600-1049-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of p70 S6 Kinase phosphorylation expression in Insulin treatedJurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Western-Blot-NB600-1049-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89544-img0011.jpg "Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining in human fallopian tube and skeletal muscle tissues . Corresponding JUNB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0016.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0013.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0014.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0012.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0015.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Chromatin-Immunoprecipitation-NBP1-30091-img0001.jpg "Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0009.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Western-Blot-NBP1-30091-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0006.jpg)
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Dot-Blot-NBP1-30091-img0002.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Western-Blot-NBP1-49546-img0008.jpg "Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.")
![Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Western-Blot-NBP1-49546-img0006.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Western-Blot-NBP1-49546-img0007.jpg)
![Dot Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Thr4]-Antibody-Dot-Blot-NBP1-49546-img0005.jpg)