| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2089AF405 | |||
| Alexa Fluor 488 | NB21-2089AF488 | |||
| Alexa Fluor 647 | NB21-2089AF647 | |||
| Alexa Fluor 700 | NB21-2089AF700 | |||
| Biotin | NB21-2089B | |||
| DyLight 405 | NB21-2089V | |||
| DyLight 488 | NB21-2089G | |||
| DyLight 550 | NB21-2089R | |||
| DyLight 650 | NB21-2089C | |||
| FITC | NB21-2089F | |||
| HRP | NB21-2089H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-92717SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81927-0.025ml | |||
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0006.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2089-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NB21-2089-img0009.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Detection of H4K20me2 in HeLa histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0003.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Dot-Blot-NB21-2089-img0001.jpg)
![Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-NB21-2089-img0010.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0011.jpg "Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunocytochemistry-Immunofluorescence-NBP2-67353-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-elF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0013.jpg)
![Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Flow-Cytometry-NBP2-67353-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0006.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0012.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of STAT3 [p Ser727] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysates, 10 ug/lane. Lane 2: NIH-3T3 cells were starved 6h, whole cell lysates, 10 ug/lane. Lane 3/4: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, whole cell lysates, 10 ug/lane. Lane 5: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, and then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. The primary antibody Anti-STAT3 [p Ser727] (1/500) , Anti-STAT3 antibody ( 1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 88 kDa Observed band size: 88 kDa](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0010.jpg "Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of STAT3 [p Ser727] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysates, 10 ug/lane. Lane 2: NIH-3T3 cells were starved 6h, whole cell lysates, 10 ug/lane. Lane 3/4: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, whole cell lysates, 10 ug/lane. Lane 5: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, and then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. The primary antibody Anti-STAT3 [p Ser727] (1/500) , Anti-STAT3 antibody ( 1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 88 kDa Observed band size: 88 kDa")
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0014.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of Phospho-STAT3(S727) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0008.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Human U251 glioma cell line, whole cell lysates.Cells were treated with SP600125, DB07268, JNK-IN-8 and SB203580. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3 (S727) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0012.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Immunohistochemistry-NB100-2371-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.")
![Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti- Phospho-Aurora A (T288) (NB100-2371) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB7159) used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo")
![Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-NBP1-97715-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0005.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human colon](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0002.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human kidney epithelium](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0001.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytes](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0003.jpg)
![Western Blot: AKT1 [p Ser473] Antibody [NB600-590] - Western Blot of AKT11 [p Ser473] antibody. Lane 1: AKT11 Recombinant Protein. Lane 2: AKT11 Mutant Human Recombinant Protein.Lane 3: AKT11 (phosphatase treated) Human Recombinant Protein. Load: 50 ng per lane.Primary antibody: AKT1 pS473 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: ~56 kDa for AKT1pS473.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Western-Blot-NB600-590-img0015.jpg "Western Blot: AKT1 [p Ser473] Antibody [NB600-590] - Western Blot of AKT11 [p Ser473] antibody. Lane 1: AKT11 Recombinant Protein. Lane 2: AKT11 Mutant Human Recombinant Protein.Lane 3: AKT11 (phosphatase treated) Human Recombinant Protein. Load: 50 ng per lane.Primary antibody: AKT1 pS473 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: ~56 kDa for AKT1pS473.")
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody [NB600-590] - Cardiomyocytes infected with adenovirus expressing wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Immunocytochemistry-Immunofluorescence-NB600-590-img0019.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody [NB600-590] - Immunohistochemistry of AKT11 [p Ser473] antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 1:100 for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Immunohistochemistry-NB600-590-img0018.jpg)
![Western Blot: AKT1 [p Ser473] Antibody [NB600-590] - Lane 1: nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 ug per lane. Primary antibody: AKT pS473 antibody at 1:200 dilution for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT pS473. Other band(s): unspecific.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Western-Blot-NB600-590-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser473] Antibody [NB600-590] - The PI3K activator peptide PTD4-PI3KAc activates AKT and induces phosphorylation of GSK3 at S9.A. SH-SY5Y cell cultures were serum starved for 16 hours and challenged afterwards with PTD4-PI3KAc 21 uM, EGF 10 ng/mL or PTD4 (control peptide at a concentration of 21 uM). Cells were lysated at the indicated times and membranes incubated with anti-p-S473AKT and anti-p-S9GSK3beta. Levels of phosphorylation were normalized to AKT and GSK3beta (lower band), respectively. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0118475), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Western-Blot-NB600-590-img0012.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody [NB600-590] - Immunohistochemistry of AKT1 [p Ser473] antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 100 dilution for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Immunohistochemistry-NB600-590-img0016.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody [NB600-590] - Immunohistochemistry at higher magnification of AKT1 [p Ser473] antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 100 dilution for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Immunohistochemistry-NB600-590-img0017.jpg)
![Dot Blot: AKT1 [p Ser473] Antibody [NB600-590]](http://images.novusbio.com/fullsize/AKT1-[p-Ser473]-Antibody-Dot-Blot-NB600-590-img0013.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.Lane 1: ACTN4 transfected lysate(104.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0021.jpg "Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.Lane 1: ACTN4 transfected lysate(104.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Immunocytochemistry-Immunofluorescence-H00000081-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human lung adenocarcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Immunohistochemistry-Paraffin-H00000081-M01-img0014.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in PC-12.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0016.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10 Analysis of ACTN4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0017.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0018.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in MCF-7.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0019.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0020.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Immunohistochemistry-Paraffin-H00000081-M01-img0012.jpg)
![ELISA: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Detection limit for recombinant GST tagged ACTN4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-ELISA-H00000081-M01-img0015.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human colon carcinoma, using Glucocorticoid receptorantibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Immunohistochemistry-Paraffin-NBP2-42221-img0006.jpg "Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human colon carcinoma, using Glucocorticoid receptorantibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunoprecipitation: GR/NR3C1 Antibody [NBP2-42221] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid Receptor beta protein by 2. 5 ug of Glucocorticoid Receptor beta antibody 7. 5% SDS-PAGE The immunoprecipitated Glucocorticoid Receptor beta protein was detected by Glucocorticoid Receptor beta antibody diluted at 1:1000](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Immunoprecipitation-NBP2-42221-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody [NBP2-42221] - GR/NR3C1 antibody detects GR/NR3C1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GR/NR3C1 stained by GR/NR3C1 antibody (NBP2-42221) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-42221_rabbit-polyclonal-gr-nr3c1-antibody-91020242075943.jpg)
![Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Western-Blot-NB100-2312-img0016.jpg "Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXO1a antibody NB100-2312 (lot NB100-2312-3) used for IP at 6 ug per reaction. FOXO1a was also immunoprecipitated by a previous lot of this antibody (lot NB100-2312-2). For blotting immunoprecipitated FOXO1a, NB100-2312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunoprecipitation-NB100-2312-img0015.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S2p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Chromatin-Immunoprecipitation-NBP2-59215-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Cross reactivity of the antibody directed against Pol IIS2p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS2p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S2 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-ELISA-NBP2-59215-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Immunofluorescence-NBP2-59215-img0005.jpg)
![Western Blot: Survivin [p Ser20] Antibody [NB110-92717] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using [NB110-92717]. Band detected at slightly higher than the predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-[p-Ser20]-Antibody-Western-Blot-NB110-92717-img0002.jpg "Western Blot: Survivin [p Ser20] Antibody [NB110-92717] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using [NB110-92717]. Band detected at slightly higher than the predicted molecular weight of 16 kDa.")
![Immunocytochemistry/Immunofluorescence: Survivin [p Ser20] Antibody [NB110-92717] - Survivin [p Ser20] Antibody (NB110-92717) was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Survivin-[p-Ser20]-Antibody-Immunocytochemistry-Immunofluorescence-NB110-92717-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-2342024155517.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.")
![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunofluorescent staining in MCF7 cells.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-234202415232510.jpg)