| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-61100-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97475-0.2mg | |||
| BSA Free | NBP1-97475-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2011AF405 | |||
| Alexa Fluor 488 | NB21-2011AF488 | |||
| Alexa Fluor 647 | NB21-2011AF647 | |||
| Alexa Fluor 700 | NB21-2011AF700 | |||
| Biotin | NB21-2011B | |||
| DyLight 405 | NB21-2011V | |||
| DyLight 488 | NB21-2011G | |||
| DyLight 550 | NB21-2011R | |||
| DyLight 650 | NB21-2011C | |||
| FITC | NB21-2011F | |||
| HRP | NB21-2011H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2011SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72631 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64808-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73181 | |||
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Western Blot: c-Myb Antibody [NBP1-80306] - WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/c-Myb-Antibody-Western-Blot-NBP1-80306-img0005.jpg "Western Blot: c-Myb Antibody [NBP1-80306] - WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate")
![Immunohistochemistry-Paraffin: c-Myb Antibody [NBP1-80306] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/c-Myb-Antibody-Immunohistochemistry-Paraffin-NBP1-80306-img0003.jpg)
![Western Blot: c-Myb Antibody [NBP1-80306] - c-Myb in MOLT-4 cells transfected with siRNA. Image from verified customer review.](http://images.novusbio.com/fullsize/c-Myb-Antibody-Western-Blot-NBP1-80306-img0004.jpg)
![Western Blot: c-Myb Antibody [NBP1-80306] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/c-Myb-Antibody-Western-Blot-NBP1-80306-img0001.jpg)
![Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/Aquaporin-2-[p-Ser261]-Antibody-Western-Blot-NB100-61100-img0001.jpg "Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-97475-img0002.jpg "Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.")
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S5p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter and the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Chromatin-Immunoprecipitation-NBP2-59219-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Cross reactivity of the antibody directed against Pol IIS5p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS5p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S5 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-ELISA-NBP2-59219-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Immunofluorescence-NBP2-59219-img0005.jpg)
![Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-Western-Blot-NB21-2011-img0002.jpg "Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2011-img0005.jpg)
![Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-NB21-2011-img0006.jpg)
![Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Western-Blot-NBP1-32213-img0030.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NBP1-32213] - Staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunohistochemistry-Frozen-NBP1-32213-img0011.jpg)
![Immunoprecipitation: p27/Kip1 Antibody [NBP1-32213] - Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 ug of p27 Kip1 antibody. Western blot analysis was performed using p27 Kip1 antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunoprecipitation-NBP1-32213-img0023.jpg)
![Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (NBP1-32213) diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp1-32213_rabbit-polyclonal-p27-kip1-antibody-western-blot-512024112649..jpg)
![Western Blot: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2D.](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Western-Blot-NBP2-45812-img0015.jpg "Western Blot: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2D.")
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0014.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0001.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0002.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0003.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0004.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0005.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0006.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0007.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0008.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0009.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0010.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0011.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0012.jpg)
![Immunohistochemistry: MEF2D Antibody (1E11) [NBP2-45812] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MEF2D-Antibody-1E11-Immunohistochemistry-NBP2-45812-img0013.jpg)
![ELISA: MEF2D Antibody (OTI1E11) [NBP2-45812] - Lysates from NIH3T3 and SVG-A cells assessed by ELISA, loading the indicated mass of total protein per well. Antibody was diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.](http://images.novusbio.com/fullsize/MEF2D-Antibody-OTI1E11-ELISA-NBP2-45812-img0017.jpg)
![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. High power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0004.jpg "Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. High power.")
![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Low power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0001.jpg)
![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0002.jpg)
![Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Medium power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunohistochemistry-NB100-64808-img0003.jpg)
![Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunofluorescence-NB100-64808-img0005.jpg)
![Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunofluorescence-NB100-64808-img0006.jpg)
![Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2, red in A and Mouse anti Human CD21, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.](http://images.novusbio.com/fullsize/LRP-1-Antibody-A2Mr-alpha-2-Immunofluorescence-NB100-64808-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Reduced EHD1 recruitment to endosomes upon EHD4 knock-down.A-F, Representative micrographs & insets depicting EHD1-GFP recruitment to endosomes in untreated (A & inset in D), mock-treated (B & inset in E), & EHD4 knock-down (C & inset in F) cells. CRISPR/Cas9 gene-edited NIH3T3 cells expressing endogenous levels of EHD1 with GFP fused to the C-terminus (EHD1-GFP) were either untreated, mock-treated with transfection reagent, or transfected with EHD4 siRNA for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed, & imaged via confocal microscopy. G, Immunoblot showing reduced EHD4 (but not EHD1-GFP) expression in EHD1-GFP cells, with actin used as a loading control. The nitrocellulose filter paper was then stripped & immunoblotted with anti-GFP to show EHD1-GFP expression upon EHD4 loss. H, Graph depicting the mean count of EHD1-labeled endosomes in untreated, mock-treated & EHD4-depleted cells. Individual experiments were performed 3 times. Error bars denote standard deviation & p-values were determined by one-way ANOVA for individual experiments using a post-hoc Tukey HSD calculator to determine significance. A consensus p-value was then derived as described in the Materials & methods to assess significant differences between samples from the 3 experiments. Micrographs are representative orthogonal projects from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment Bar, 10 μm. n.s., not significant (consensus p > 0.5). Consensus p-values from Tukey HSD: *p < 0.00001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-64808_mouse-monoclonal-lrp-1-antibody-a2mr-alpha-2-310202415541917.jpg)
![Immunocytochemistry/ Immunofluorescence: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Increased sorting endosome size & decreased EHD1 recruitment upon Rabenosyn-5 knock-down.A-L, Representative micrographs & insets depicting EEA1-labeled endosomes & EHD1-GFP in mock-treated & Rabenosyn-5 knock-down cells. CRISPR/Cas9 gene-edited NIH3T3 EHD1-GFP cells were either mock-treated with transfection reagent (A-F) or treated with Rabenosyn-5 siRNA (G-L) for 72 h. Cells were then incubated with anti-LRP1 antibody (30 min on ice, 30 min at 37°C), fixed & immunostained using anti-EEA1, & imaged by confocal microscopy. M, Immunoblot showing reduced Rabenosyn-5 expression in EHD1-GFP NIH3T3 cells. N, Graph depicting mean endosome size of mock-treated & Rabenosyn-5 knock-down cells. O, Graph depicting EHD1 recruitment to endosomes in mock-treated & Rabenosyn-5 knock-down cells. Error bars denote standard deviation & p-values were determined by independent two-tailed t-test, with significance derived from consensus p-values from the 3 experiments. Micrographs are representative orthogonal projections from three independent experiments, with 10 sets of z-stacks collected for each treatment per experiment. Bar, 10 μm. **p < 0.00001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32966336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-64808_mouse-monoclonal-lrp-1-antibody-a2mr-alpha-2-31020241682149.jpg)
![Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Western-Blot-NBP2-00532-img0009.jpg "Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Immunocytochemistry-Immunofluorescence-NBP2-00532-img0008.jpg)
![Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Immunohistochemistry-Paraffin-NBP2-00532-img0007.jpg)
![Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Flow-Cytometry-NBP2-00532-img0003.jpg)
![Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Western-Blot-NBP2-00532-img0004.jpg)
![Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Immunohistochemistry-Paraffin-NBP2-00532-img0005.jpg)
![Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Immunohistochemistry-Paraffin-NBP2-00532-img0006.jpg)
![Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Flow-Cytometry-NBP2-00532-img0001.jpg)
![Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/BCAR1-Antibody-1A9-Flow-Cytometry-NBP2-00532-img0002.jpg)
![Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Western-Blot-NBP1-22439-img0015.jpg "Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0018.jpg)
![Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (NBP1-22439) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunohistochemistry-NBP1-22439-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0019.jpg)
![Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunohistochemistry-NBP1-22439-img0016.jpg)
![Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - ICC staining of Phospho-JAK2(Y1007+Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor (TM) 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Western-Blot-NBP2-67429-img0006.jpg "Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - ICC staining of Phospho-JAK2(Y1007+Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor (TM) 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Staining Phospho-JAK2(Y1007+Y1008) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunocytochemistry-Immunofluorescence-NBP2-67429-img0001.jpg)
![Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Analysis of paraffin-embedded mouse kidney tissue using anti-Jak2 [p Tyr1007, p Tyr1008] antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0007.jpg)
![Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0002.jpg)
![Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Human tonsil tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0003.jpg)
![Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Human kidney tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0004.jpg)
![Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Jak2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0005.jpg)