| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61843-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61894-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38798-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84687-25ul | |||
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0005.jpg "Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunocytochemistry-Immunofluorescence-NBP2-61843-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of cervical cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunohistochemistry-NBP2-61843-img0004.jpg)
![Flow Cytometry: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0002.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0006.jpg)
![ELISA: mGluR3 Antibody (7A5A6) [NBP2-61843] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0001.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0007.jpg "Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0005.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0006.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mouse mAb against NIH/3T3 (1), U251 (2), C6 (3), A549 (4), and MCF-7 (5) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0004.jpg)
![ELISA: Synapsin I Antibody (7B1D9) [NBP2-61894] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-ELISA-NBP2-61894-img0001.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NB300-113-img0005.jpg "Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.")
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord showing specific immunolabeling of the ~48k a1- and a2-subunits of the glycine receptor. The labeling was absent from a Rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NB300-113-img0003.jpg)
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Western blot of 10 ug of rat spinal cord. The antibody is specific for the ~48 kDa a1- and a2-subunitsof the glycine receptor.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NB300-113-img0004.jpg)
![Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: Rat brain tissue lysate](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Western-Blot-NBP2-75497-img0006.jpg "Western Blot: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: Rat brain tissue lysate")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunocytochemistry-Immunofluorescence-NBP2-75497-img0003.jpg)
![Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunohistochemistry-Paraffin-NBP2-75497-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunocytochemistry-Immunofluorescence-NBP2-75497-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunocytochemistry-Immunofluorescence-NBP2-75497-img0002.jpg)
![Immunohistochemistry-Paraffin: GABA-AR alpha 5 Antibody (JB34-19) [NBP2-75497] - Analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GABA-AR-alpha-5-Antibody-JB34-19-Immunohistochemistry-Paraffin-NBP2-75497-img0005.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Western-Blot-NBP2-67580-img0004.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate")
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-Tryptophan Hydroxylase/TPH-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0005.jpg)
![Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Flow-Cytometry-NBP2-67580-img0001.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0003.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody at 1/1,000 dilution.Lane 1: MDA-MB-231-si NT cell lysateLane 2: MDA-MB-231-si TPH1 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 51 kDaObserved band size: 72 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-67580 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, (1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67580_rabbit-monoclonal-tryptophan-hydroxylase-1-tph-1-antibody-sc53-07-western-blot-462024131133..jpg)
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15334-img0001.jpg "Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Cal27 xenograft, using ALDH1A1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15334-img0002.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15334-img0003.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE ALDH1A1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15334-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP2-15334) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15334_rabbit-polyclonal-aldehyde-dehydrogenase-1-a1-aldh1a1-antibody-9102024206330.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunocytochemistry-Immunofluorescence-NBP3-11866-img0005.jpg "Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11866. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunohistochemistry-Paraffin-NBP3-11866-img0002.jpg)
![Flow Cytometry: mGluR5 Antibody (ST51-00) [NBP3-11866] - Flow cytometric analysis of SH-SY-5Y cells with NBP3-11866 at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Flow-Cytometry-NBP3-11866-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunocytochemistry-Immunofluorescence-NBP3-11866-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunocytochemistry-Immunofluorescence-NBP3-11866-img0004.jpg)
![Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using NBP3-11866. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunohistochemistry-Paraffin-NBP3-11866-img0001.jpg)
![Immunohistochemistry-Frozen: GRID2 Antibody [NBP1-46489] - Axolotl Octavolateralis Nuclei. Image from verified customer review.](http://images.novusbio.com/fullsize/GRID2-Antibody-Immunohistochemistry-Frozen-NBP1-46489-img0001.jpg "Immunohistochemistry-Frozen: GRID2 Antibody [NBP1-46489] - Axolotl Octavolateralis Nuclei. Image from verified customer review.")
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp3-52088_mouse-mbp-mab-cl2829-western-blot-273202615204516.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp3-52088_mouse-mbp-mab-cl2829-western-blot-27320261527554.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human cerebellum, kidney, liver and lymph node using Anti-CALB1 antibody NBP2-38798 (A) shows similar protein distribution across tissues to independent antibody NBP1-86664 (B).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0005.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-38798] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-38798-img0002.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human lymph node using Anti-CALB1 antibody NBP2-38798.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0009.jpg)
![Immunohistochemistry: Calbindin D-28K Antibody [NBP2-38798] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-NBP2-38798-img0001.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0003.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0006.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human liver.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0007.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human kidney using Anti-CALB1 antibody NBP2-38798.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0008.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP1-84687-img0005.jpg "Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0020.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0008.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0011.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0012.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0013.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP1-84687 (A) shows similar protein distribution across tissues to independent antibody NBP2-38622 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP1-84687 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0018.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human pancreas shows moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0019.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0021.jpg)