| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33555-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92907-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12906-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61781-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61894-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61843-0.025ml | |||
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024941471.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415939.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024942549.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249393563.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415961.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249371590.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249355523.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0014.jpg "Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.")
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse olfactory bulb shows immunoreactivity in processes in external plexiform layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0011.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0013.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebral cortex shows positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human hippocampus shows moderate immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0006.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebellum shows moderate nuclear and cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0015.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebellum shows immunoreactivity in granular cell layer and in white matter.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0007.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebral cortex shows labeling of neuronal processes in motor cortex and in corpus callosum.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0008.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse dorsal tenia tecta shows positivity in neuronal processes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0009.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse hippocampus shows positivity in neuronal processes in the CA1 layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0010.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Staining of human (A), mouse (B) and rat (C) liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NB100-787-img0001.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Staining of human (A), mouse (B) and rat (C) liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-787_goat-polyclonal-aldehyde-dehydrogenase-1-a1-aldh1a1-antibody-12920231134475.jpg)
![Western Blot: CSN1 Antibody [NBP2-92907] - Analysis of extracts of various cell lines, using CSN1 .Exposure time: 90s.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NBP2-92907-img0005.jpg "Western Blot: CSN1 Antibody [NBP2-92907] - Analysis of extracts of various cell lines, using CSN1 .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: CSN1 Antibody [NBP2-92907] - Immunofluorescence analysis of U2OS cells using CSN1 Rabbit pAb (NBP2-92907) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92907-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CSN1 Antibody [NBP2-92907] - Immunofluorescence analysis of C6 cells using CSN1 Rabbit pAb (NBP2-92907) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92907-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: CSN1 Antibody - BSA Free [NBP2-92907] - Immunofluorescence analysis of NIH/3T3 cells using CSN1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-42202514275224.jpg)
![Immunohistochemistry: CSN1 Antibody - BSA Free [NBP2-92907] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CSN1 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-4220251431816.jpg)
![Immunohistochemistry: CSN1 Antibody - BSA Free [NBP2-92907] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using CSN1 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-42202514251852.jpg)
![Immunohistochemistry: CSN1 Antibody - BSA Free [NBP2-92907] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CSN1 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-4220251429067.jpg)
![Western Blot: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Western blot analysis of extracts of various cell lines, using VAMP-2 Rabbit mAb (NBP3-16362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Western-Blot-NBP3-16362-img0003.jpg "Western Blot: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Western blot analysis of extracts of various cell lines, using VAMP-2 Rabbit mAb (NBP3-16362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Immunofluorescence analysis of mouse eye using VAMP-2 Rabbit mAb (NBP3-16362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Immunohistochemistry-NBP3-16362-img0002.jpg)
![Western Blot: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Western blot analysis of extracts of RD cells, using VAMP-2 Rabbit mAb (NBP3-16362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Western-Blot-NBP3-16362-img0004.jpg)
![Immunohistochemistry: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Immunofluorescence analysis of rat eye using VAMP-2 Rabbit mAb (NBP3-16362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Immunohistochemistry-NBP3-16362-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Confocal imaging of paraffin-embedded Rat eye using VAMP-2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16362_rabbit-vamp-2-mab-8x9d9-52202511411424.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Confocal imaging of paraffin-embedded Mouse brain using VAMP-2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16362_rabbit-vamp-2-mab-8x9d9-52202511365148.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Confocal imaging of paraffin-embedded Mouse eye using VAMP-2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16362_rabbit-vamp-2-mab-8x9d9-52202511383720.jpg)
![Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Western-Blot-NBP2-12906-img0008.jpg "Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunocytochemistry-Immunofluorescence-NBP2-12906-img0011.jpg)
![Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunohistochemistry-NBP2-12906-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Human hippocampal tissues.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunocytochemistry-Immunofluorescence-NBP2-12906-img0004.jpg)
![Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunohistochemistry-NBP2-12906-img0009.jpg)
![Western Blot: GABA-A R alpha 6 Antibody [NB300-196] - Analysis of mouse forebrain lysates from Wild Type (Control) and a6-knockout (a6-K/O) animals showing specific immunolabeling of the ~57k a6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a6-knockout animals.](http://images.novusbio.com/fullsize/GABA-A-Receptor-alpha-6-Antibody-Western-Blot-NB300-196-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GABA-A R alpha 6 Antibody [NB300-196] - Analysis of mouse forebrain lysates from Wild Type (Control) and a6-knockout (a6-K/O) animals showing specific immunolabeling of the ~57k a6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a6-knockout animals.")
![<b>Genetic Strategies Validation. </b>Western Blot: GABA A Receptor alpha 6 Antibody [NB300-196] - Detection of GABA-A alpha 6 receptor in wildtype cerebellum (5-7 ug/lane).](http://images.novusbio.com/fullsize/GABA-A-Receptor-alpha-6-Antibody-Western-Blot-NB300-196-img0001.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.](http://images.novusbio.com/fullsize/Kv3.1-[p-Ser503]-Antibody-Immunohistochemistry-NB300-279-img0001.jpg "Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.")
![Western Blot: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-Western-Blot-NBP2-61781-img0006.jpg "Western Blot: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunocytochemistry/Immunofluorescence: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-Immunocytochemistry-Immunofluorescence-NBP2-61781-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis of bladder cancer tissues using HTR3A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-Immunohistochemistry-NBP2-61781-img0005.jpg)
![Western Blot: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-Western-Blot-NBP2-61781-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis of cervical cancer tissues using HTR3A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-Immunohistochemistry-NBP2-61781-img0004.jpg)
![ELISA: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-ELISA-NBP2-61781-img0001.jpg)
![ELISA: 5-HT3A Antibody (3B11C10) [NBP2-61781] - Analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-HT3A-Antibody-3B11C10-ELISA-NBP2-61781-img0002.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0007.jpg "Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0005.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0006.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mouse mAb against NIH/3T3 (1), U251 (2), C6 (3), A549 (4), and MCF-7 (5) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0004.jpg)
![ELISA: Synapsin I Antibody (7B1D9) [NBP2-61894] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-ELISA-NBP2-61894-img0001.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0005.jpg "Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunocytochemistry-Immunofluorescence-NBP2-61843-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of cervical cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunohistochemistry-NBP2-61843-img0004.jpg)
![Flow Cytometry: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0002.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0006.jpg)
![ELISA: mGluR3 Antibody (7A5A6) [NBP2-61843] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0001.jpg)