| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68136SS | |||
| Azide and BSA Free | NBP2-80973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47254SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56883SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86048-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37446-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13464-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38650-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89639-25ul | |||
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Intracellular-NB110-68136-img0010.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].")
![<b>Biological Strategies Validation. </b>Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-BSA-Free-Flow-Cytometry-NB110-68136-img0016.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0008.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0007.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0011.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0013.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0012.jpg "Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0009.jpg)
![Immunohistochemistry-Paraffin: Gremlin 1 Antibody [NB100-56883] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Gremlin-1-Antibody-Immunohistochemistry-Paraffin-NB100-56883-img0002.jpg "Immunohistochemistry-Paraffin: Gremlin 1 Antibody [NB100-56883] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml.")
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0006.jpg "Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0004.jpg)
![Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Flow-Cytometry-NBP2-67216-img0001.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0003.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining in human thyroid gland and skeletal muscle tissues . Corresponding CTSB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-86048-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining in human thyroid gland and skeletal muscle tissues . Corresponding CTSB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cathepsin B Antibody [NBP1-86048] - Staining of human cell line A-431 shows localization to nucleoli & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86048-img0010.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human thyroid gland shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-86048-img0018.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human cerebral cortex shows granular cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-86048-img0015.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human lung shows cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-86048-img0016.jpg)
![Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cathepsin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-86048-img0017.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Western-Blot-NBP3-16524-img0003.jpg "Western Blot: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry of paraffin-embedded mouse liver using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Immunohistochemistry-Paraffin-NBP3-16524-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry of paraffin-embedded human liver cancer using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Immunohistochemistry-Paraffin-NBP3-16524-img0001.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded mouse kidney tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512432081.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded rat brain tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512435344.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded human liver tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-722025124532.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded human liver cancer tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512435325.jpg)
![Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Western-Blot-NBP2-37446-img0003.jpg "Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37446-img0004.jpg)
![Immunohistochemistry-Paraffin: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Analysis of ovarian cancer tissues using HAS2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Immunohistochemistry-NBP2-37446-img0005.jpg)
![Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Western-Blot-NBP2-37446-img0002.jpg)
![ELISA: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-ELISA-NBP2-37446-img0001.jpg)
![Immunohistochemistry-Paraffin: TOX2 Antibody [NBP2-13464] - Staining in human lymph node and skeletal muscle tissues.. Corresponding TOX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-13464-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TOX2 Antibody [NBP2-13464] - Staining in human lymph node and skeletal muscle tissues.. Corresponding TOX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TOX2 Antibody [NBP2-13464] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TOX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13464-img0003.jpg)
![Immunohistochemistry-Paraffin: TOX2 Antibody [NBP2-13464] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/TOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-13464-img0006.jpg)
![Immunohistochemistry-Paraffin: TOX2 Antibody [NBP2-13464] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/TOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-13464-img0004.jpg)
![Immunohistochemistry-Paraffin: TOX2 Antibody [NBP2-13464] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/TOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-13464-img0005.jpg)
![Western Blot: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Western blot analysis of Drebrin 1 on different lysates with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: AD Mouse brain tissue lysate (low expression) Lane 6: Rat brain tissue lysate Lane 7: PC-12 cell lysate Lane 8: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 110 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-278202415242654.jpg "Western Blot: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Western blot analysis of Drebrin 1 on different lysates with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: AD Mouse brain tissue lysate (low expression) Lane 6: Rat brain tissue lysate Lane 7: PC-12 cell lysate Lane 8: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 110 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32279) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-29820241435255.jpg)
![Immunocytochemistry/ Immunofluorescence: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Immunocytochemistry analysis of SH-SY5Y cells labeling Drebrin 1 with Rabbit anti-Drebrin 1 1 antibody (NBP3-32279) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-29202410484899.jpg)
![Flow Cytometry: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Flow cytometric analysis of SH-SY5Y cells labeling Drebrin 1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32279, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-392024884613.jpg)
![Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody [NBP2-20315] - Human gastric cancer, using SERPINE2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SERPINE2-Antibody-Immunohistochemistry-Paraffin-NBP2-20315-img0003.jpg "Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody [NBP2-20315] - Human gastric cancer, using SERPINE2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Serpin E2/PN1 Antibody [NBP2-20315] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-Western-Blot-NBP2-20315-img0010.jpg)
![Immunohistochemistry-Paraffin: Drebrin 1 Antibody [NBP2-38650] -Analysis in human cerebral cortex and liver tissues using NBP2-38650 antibody. Corresponding DBN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-38650_rabbit-polyclonal-drebrin-1-antibody-immunohistochemistry-paraffin-2110202412327..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Drebrin 1 Antibody [NBP2-38650] -Analysis in human cerebral cortex and liver tissues using NBP2-38650 antibody. Corresponding DBN1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Drebrin 1 Antibody [NBP2-38650] - Staining of human cerebral cortex, kidney, pancreas and testis using Anti-DBN1 antibody NBP2-38650 (A) shows similar protein distribution across tissues to independent antibody NBP2-38813 (B).](http://images.novusbio.com/fullsize/Drebrin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38650-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Drebrin 1 Antibody [NBP2-38650] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.](http://images.novusbio.com/fullsize/Drebrin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38650-img0004.jpg)
![Immunohistochemistry-Paraffin: Drebrin 1 Antibody [NBP2-38650] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Drebrin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38650-img0005.jpg)
![Immunohistochemistry-Paraffin: Drebrin 1 Antibody [NBP2-38650] - Staining of human testis shows strong membranous positivity in spermatogonia.](http://images.novusbio.com/fullsize/antibody/nbp2-38650_rabbit-polyclonal-drebrin-1-antibody-immunohistochemistry-paraffin-21102024123355..jpg)
![Immunohistochemistry-Paraffin: Drebrin 1 Antibody [NBP2-38650] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp2-38650_rabbit-polyclonal-drebrin-1-antibody-immunohistochemistry-paraffin-21102024123459..jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody [NBP1-89639] - Staining of human hippocampus shows distinct positivity in neuropil.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-89639-img0002.jpg "Immunohistochemistry-Paraffin: Tenascin C Antibody [NBP1-89639] - Staining of human hippocampus shows distinct positivity in neuropil.")