| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-540SS | |||
| Azide and BSA Free | NBP2-80677 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-140-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87679-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81007-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92886-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61855-0.025ml | |||
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Flow-Intracellular-NB200-540-img0005.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Flow-Cytometry-NB200-540-img0006.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0003.jpg)
![Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - Analysis of different tissue lysates using alpha-Internexin, dilution 1:10000 (Red): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66kDa, with some variability across species.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Western-Blot-NB300-140-img0005.jpg "Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - Analysis of different tissue lysates using alpha-Internexin, dilution 1:10000 (Red): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66kDa, with some variability across species.")
![Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140] - Hippocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB300-140 and GFAP (green), using NB300-141.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Immunocytochemistry-Immunofluorescence-NB300-140-img0003.jpg)
![Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140] - Section of rat facial nucleus 7 days following axotomy. These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate alpha-Internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of alpha-Internexin. Both findings suggest that alpha-Internexin has a role in axonal regeneration.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Immunohistochemistry-NB300-140-img0006.jpg)
![Simple Western: alpha-Internexin Antibody (2E3) [NB300-140] - Simple Western lane view shows a specific band for alpha Internexin in 0.5 mg/ml of SH-SY5Y lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Simple-Western-NB300-140-img0004.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0027.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human brain tissue.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP1-87679-img0017.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679] - Staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0029.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebellum shows moderate to strong membranous positivity in astrocytes, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0030.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0031.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human liver shows no positivity in hepatocytes, as expected.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0032.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0033.jpg)
![Immunohistochemistry-Frozen: Aquaporin-4 Antibody [NBP1-87679] - Mouse trigeminal ganglion stained with Aquaporin-4 antibody at 1:5000. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Frozen-NBP1-87679-img0036.jpg)
![Induction of atrogin 1 mRNA and protein expression by rotator cuff tear (RCT). (a) Expression of transcripts encoding the E3 ubiquitin-protein ligases atrogin 1, MuRF1, and UBR2 in injured muscle after RCT (n = 12 per group). (b) Atrogin 1 protein expression in muscle isolated at different stages after RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (c) Immunofluorescence (IF) microscopy analyses of muscle isolated at different stages after RCT. The red signal represents atrogin 1 protein. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). (d) Loss of AQP4 protein by overexpression of atrogin 1. C2C12 cells were treated with an adenovirus expressing atrogin 1 [Ad-atrogin 1; multiplicity of infection (MOI) = 50, 100, 200] for 48 h, and western blot analysis was performed with each designated antibody. (e) Monomeric ubiquitin protein expression in muscle cells isolated at different stages after RCT. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87679_rabbit-polyclonal-aquaporin-4-antibody-western-blot-13220261319313.jpg)
![Increased levels of HMGB1 protein in muscle injured by rotator cuff tear (RCT). (a) Expression level of HMGB1 protein in muscle isolated at different stages after RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (b) Immunofluorescence (IF) microscopy analyses of muscle cells isolated at different stages after RCT. The green signal represents HMGB1 protein. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). (c) Expression of atrogin 1 mRNA in C2C12 myotubes treated with 200 ng/mL recombinant mouse HMGB1 for the indicated time periods. (d) Atrogin 1, ubiquitin, and AQP4 protein levels in C2C12 myotubes treated with 200 ng/mL HMGB1 and an adenovirus expressing atrogin 1 [Ad-atrogin 1; multiplicity of infection (MOI) = 100] for 48 h. (e) Ubiquitination of AQP4 caused by HMGB1 and atrogin 1. C2C12 myotubes were treated with 200 ng/mL HMGB1 and Ad-atrogin 1 (MOI = 100) with or without MG132. After 48 h of incubation, cell lysates were prepared for a ubiquitination assay using anti-AQP4 and anti-ubiquitin antibodies. (f) Expression of atrogin 1 mRNA in C2C12 myotubes exposed to 5 μM SB203580 (a p38 MAPK inhibitor), LY294002 (a PI3K inhibitor), BAY 11-7082 (an NF-kappa B inhibitor), PD98059 (an MEK inhibitor), or SP600125 (a JNK inhibitor). Cells were exposed to each inhibitor for 1 h prior to treatment with 200 ng/mL HMGB1 for 24 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87679_rabbit-polyclonal-aquaporin-4-antibody-western-blot-132202613153820.jpg)
![Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunofluorescence-NB300-139-img0006.jpg "Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.")
![Western Blot: alpha-Internexin Antibody [NB300-139] - Whole tissue lysates using rabbit pAb to alpha-internexin, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0007.jpg)
![Western Blot: alpha-Internexin Antibody [NB300-139] - The expression of a-internexin (INA) was analyzed by Western blotting. White bars are controls, grey bars are enucleated and black bars are anophthalmic mice. Results from V1 (left), V2M (middle) and V2L (right) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159320) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - IF Confocal analysis of SHSY5Y cells using alpha Internexin antibody (NB300-139, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0005.jpg)
![Western Blot: alpha Internexin Antibody [NB300-139] - Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kDa is also seen, apparently resulting from dimerization of alpha-internexin.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin CPCA-Peri (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Polyclonal alpha internexin, NB 300-139 (red) staining neuronal progenitor cells. The green stain shows the fibroblast marker, Plectin (not one of our antibodies).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0004.jpg)
![Western Blot: alpha-Internexin Antibody [NB300-139] - Proteins involved in morphogenesis.The expression of a-internexin (INA), collapsing response mediator protein 2 (CRMP2) & 4 (CRMP4), neurofilament-low (NFL) & tubuline-b2 (Tub beta 2) were analyzed by Western blotting. White bars are controls, grey bars are enucleated & black bars are anophthalmic mice. Results from V1 (left), V2M (middle) & V2L (right) are shown for each protein. * P < 0.05 & ** P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27410964), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-139_rabbit-polyclonal-alpha-internexin-antibody-310202415371950.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0023.jpg "Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32080-img0019.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Mouse brain. C3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-32080-img0015.jpg)
![Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunoprecipitation-NBP1-32080-img0017.jpg)
![Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Complement C3 antibody [C3], C-term (NBP1-32080) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32080_rabbit-polyclonal-complement-c3-antibody-western-blot-1411202518442224.jpg)
![Western Blot: alpha-Internexin Antibody [NBP1-81007] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NBP1-81007-img0014.jpg "Western Blot: alpha-Internexin Antibody [NBP1-81007] - Analysis in human cell line SH-SY5Y.")
![Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NBP1-81007] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, nuclear membrane & intermediate filaments.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81007-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining in human cerebral cortex and kidney tissues . Corresponding INA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Paraffin-NBP1-81007-img0020.jpg)
![Western Blot: alpha-Internexin Antibody [NBP1-81007] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NBP1-81007-img0004.jpg)
![Western Blot: alpha-Internexin Antibody [NBP1-81007] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NBP1-81007-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining of mouse brain shows moderate positivity in of in the hippocampus.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Paraffin-NBP1-81007-img0015.jpg)
![Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining of mouse brain shows positivity in the cerebral cortex.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Paraffin-NBP1-81007-img0016.jpg)
![Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining of human cerebellum shows positivity in purkinje cells.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Paraffin-NBP1-81007-img0017.jpg)
![Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining of human cerebral cortex shows positivity in neuropil.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Paraffin-NBP1-81007-img0018.jpg)
![Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining of human kidney shows very weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Paraffin-NBP1-81007-img0019.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Capicua Antibody [NBP2-33420] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CIC antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Capicua Antibody [NBP2-33420] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Capicua Antibody [NBP2-33420] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Staining is shown in green.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33420-img0012.jpg)
![Western Blot: Capicua Antibody [NBP2-33420] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0005.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human cerebellum shows strong nuclear immunoreactivity in Purkinje cells, as well as positivity in neurons in granular and molecular layers.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0007.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular stromal cells.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0008.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0009.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0010.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Western Blot-NBP2-92886-img0003.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded mouse brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92886_rabbit-polyclonal-aquaporin-4-antibody-42202514264496.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92886_rabbit-polyclonal-aquaporin-4-antibody-4220251430473.jpg)
![Western Blot: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Western blot analysis of extracts of various cell lines, using alpha-Internexin Rabbit mAb (NBP3-16230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-5E5Z3-Western-Blot-NBP3-16230-img0006.jpg "Western Blot: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Western blot analysis of extracts of various cell lines, using alpha-Internexin Rabbit mAb (NBP3-16230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunohistochemistry analysis of alpha-Internexin in paraffin-embedded mouse brain tissue using alpha-Internexin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-7220251245386.jpg)
![Immunohistochemistry: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunohistochemistry analysis of alpha-Internexin in paraffin-embedded human appendix tissue using alpha-Internexin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-72202512432059.jpg)
![Immunohistochemistry: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunohistochemistry analysis of alpha-Internexin in paraffin-embedded rat brain tissue using alpha-Internexin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-7220251245333.jpg)
![Immunohistochemistry: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunohistochemistry analysis of alpha-Internexin in paraffin-embedded human colon tissue using alpha-Internexin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-72202512435377.jpg)
![Immunohistochemistry: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunohistochemistry analysis of alpha-Internexin in paraffin-embedded human brain tissue using alpha-Internexin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-72202512443349.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunofluorescence analysis of paraffin-embedded mouse brain using alpha-Internexin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-8220252010478.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody (5E5Z3) [NBP3-16230] - Immunofluorescence analysis of paraffin-embedded rat brain using alpha-Internexin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16230_rabbit-alpha-internexin-mab-5e5z3-822025209831.jpg)
![Western Blot: Complement C3 Antibody (4C6A8) [NBP2-61855] - Analysis using C3C mAb against human C3C (AA: 1521-1649) recombinant protein. (Expected MW is 40.7 kDa)](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-Western-Blot-NBP2-61855-img0006.jpg "Western Blot: Complement C3 Antibody (4C6A8) [NBP2-61855] - Analysis using C3C mAb against human C3C (AA: 1521-1649) recombinant protein. (Expected MW is 40.7 kDa)")
![Immunocytochemistry/Immunofluorescence: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of Hela cells using C3C mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-Immunocytochemistry-Immunofluorescence-NBP2-61855-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of paraffin-embedded bladder cancer tissues using C3C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-Immunohistochemistry-NBP2-61855-img0005.jpg)
![Flow Cytometry: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of Hela cells using C3C mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-Flow-Cytometry-NBP2-61855-img0002.jpg)
![Western Blot: Complement C3 Antibody (4C6A8) [NBP2-61855] - Analysis using C3C mAb against HEK293 (1) and C3C (AA: 1521-1649)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-Western-Blot-NBP2-61855-img0007.jpg)
![Immunohistochemistry-Paraffin: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of paraffin-embedded ovarian cancer tissues using C3C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-Immunohistochemistry-NBP2-61855-img0004.jpg)
![ELISA: Complement C3c Antibody (4C6A8) [NBP2-61855] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Complement-C3-Antibody-4C6A8-ELISA-NBP2-61855-img0001.jpg)
![ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Samples were spiked with high concentrations of Mouse Complement C3a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70037_mouse-complement-c3a-elisa-kit-colorimetric-13220241626433.jpg "ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Samples were spiked with high concentrations of Mouse Complement C3a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70037_mouse-complement-c3a-elisa-kit-colorimetric-432024941599.jpg)
![Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot analysis of different tissue lysates using chicken pAb to alpha-Internexin, CPCA-a-Int, dilution 1:10,000 in green: (1) protein standard (red), (2) rat brain, (3) rat spinal cord, (4) mouse brain, (5) mouse spinal cord, (6) cow spinal cord and (7) pig spinal cord. CPCA-a-Int antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NBP1-05208-img0006.jpg "Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot analysis of different tissue lysates using chicken pAb to alpha-Internexin, CPCA-a-Int, dilution 1:10,000 in green: (1) protein standard (red), (2) rat brain, (3) rat spinal cord, (4) mouse brain, (5) mouse spinal cord, (6) cow spinal cord and (7) pig spinal cord. CPCA-a-Int antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.")
![Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NBP1-05208] - Mixed neuron glia cultures stain with NBP1-05208 (red) and counterstained with RPCA-Cor1a, EnCor's rabbit polyclonal antibody to Coronin 1a, (green) which is an excellent marker of microglia and lymphocytes. The chicken internexin antibody is an excellent marker of neuronal processes in these cultures.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05208-img0004.jpg)
![Immunohistochemistry Free-Floating: alpha-Internexin Antibody [NBP1-05208] - Analysis of a rat cerebellum section stained with chicken alpha-Internexin pAb , dilution 1:5,000 (red), and costained with mouse MBP mAb, 1:5,000 (Green). Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The alpha-Internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunohistochemistry-Free-Floating-NBP1-05208-img0007.jpg)
![Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot of extract of rat brain stem crude extract stained with NBP1-05208, showing a single strong clean band at approx. 66kDa.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NBP1-05208-img0005.jpg)