| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33066 | |||
| IHC-Prediluted | NBP2-44365 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90080-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47946-0.1mg | |||
| IHC-Prediluted | NBP2-48095 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87334-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59320-25ug | |||
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0017.jpg "Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of human hepatoma. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of mouse skin. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of rat hind brain. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0003.jpg)
![Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Glutamine synthetase antibody [1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [1055] diluted at 1:500. Blue: DAPI](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Frozen-NBP2-43646-img0009.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-261120251820022.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-2611202518255016.jpg)
![Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-immunohistochemistry-frozen-2611202518333613.jpg)
![Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-western-blot-261120251826138.jpg)
![Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (NBP2-43646) diluted at 1:250.Red: GFAP, stained by GFAP antibody diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43646_mouse-monoclonal-glutamine-synthetase-antibody-1055-immunocytochemistry-immunofluorescence-2611202519365.jpg)
![Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Western-Blot-NBP2-29439-img0011.jpg "Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0010.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Mouse Monoclonal Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0012.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin paraffin breast cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-paraffin ovarian cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0004.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0008.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0009.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - U87 xenograft, using GSTP1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16756-img0014.jpg)
![GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSTP1 protein stained by GSTP1 antibody (NBP2-16756) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16756_rabbit-polyclonal-glutathione-s-transferase-pi-1-gstp1-antibody-immunocytochemistry-immunofluorescence-2611202518283519.jpg)
![Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-89125-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Nrf1 Antibody [NBP1-89125] - The SIAH2-NRF1 axis facilitates hypoxia-induced metabolic reprogramming. Wild-type or K230R stably expressed MDA-MB-231 cells were cultured under normoxia or hypoxia for 36 h. Cells were harvested and analyzed by western blotting with indicated antibodies. Right: densitometric quantification of the indicated proteins. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-08618-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-89125-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP1-89125] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89125-img0005.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0011.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0009.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human cerebral cortex shows moderate positivity.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0013.jpg)
![Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0014.jpg)
![Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0012.jpg)
![Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0015.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Fetal Brain Lane A: Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/Lane Gel Concentration: 0.12](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0015.jpg "Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Fetal Brain Lane A: Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/Lane Gel Concentration: 0.12")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59632] - Human alveolar cell.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59632-img0011.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0012.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - WB analysis of GLT1 in Hep3B WCL. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0013.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Human Fetal Brain lysates.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0014.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59632] - Human Brain, cortex tissue at an antibody concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59632-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: EDNRB/Endothelin R Type B Antibody [NLS54] - Analysis of anti-EDNRB / Endothelin B Receptor antibody with transfected cells expressing Endothelin B Receptor at 4 ug/ ml.](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0003.jpg "Immunocytochemistry/Immunofluorescence: EDNRB/Endothelin R Type B Antibody [NLS54] - Analysis of anti-EDNRB / Endothelin B Receptor antibody with transfected cells expressing Endothelin B Receptor at 4 ug/ ml.")
![Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Brain, Alzheimer's senile plaque](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: EDNRB/Endothelin R Type B Antibody [NLS54] - Analysis of anti-EDNRB / Endothelin B Receptor antibody with cells expressing vector only using this antibody at 4 ug/ ml.](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0002.jpg)
![Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Analysis of anti-EDNRB / Endothelin B Receptor antibody with adrenal, medulla at 4 ug/ ml.](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0001.jpg)
![Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Analysis of anti-EDNRB / Endothelin B Receptor antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0004.jpg)
![Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Human Lung, Small Cell Carcinoma of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0005.jpg)
![Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Brain, Dementia, Senile Plaque](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0006.jpg)
![Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Artery, smooth muscle](http://images.novusbio.com/fullsize/EDNRB-Endothelin-R-Type-B-Antibody-Immunohistochemistry-Paraffin-NLS54-img0007.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0007.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33134-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0004.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Breast cancer tissue stained for Cytokeratin 19 (red) counter stained with DAPI (blue). The Janelia-549 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-33134-img0001.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0002.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0003.jpg)
![Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Western-Blot-NBP2-44827-img0012.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (KRT19/800) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunocytochemistry-Immunofluorescence-NBP2-44827-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0008.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87334-img0028.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining in human cerebral cortex and liver tissues . Corresponding UCHL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0027.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0017.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NBP1-87334-img0018.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons and in neuropil.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0022.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0023.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human liver shows no positivity in hepatocytes, as expected.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0024.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0025.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglion and peripheral nerves.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunohistochemistry-Paraffin-NBP1-87334-img0026.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0007.jpg "Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-59320_mouse-monoclonal-sur1-antibody-s289-16-310202416114773.jpg)