| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03937-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02943-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61856-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42190-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62728-25ul | |||
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).")
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human prostate. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0002.jpg)
![Flow Cytometry: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TRP1-Antibody-Flow-Cytometry-NBP2-62000-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0004.jpg)
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human cortex. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0001.jpg)
![Western Blot: Dishevelled-3 Antibody [NBP3-03068] - Western blot analysis of extracts of various cell lines, using Dishevelled-3 antibody (NBP3-03068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Dishevelled-3 Antibody-Western Blot-NBP3-03068-img0005.jpg "Western Blot: Dishevelled-3 Antibody [NBP3-03068] - Western blot analysis of extracts of various cell lines, using Dishevelled-3 antibody (NBP3-03068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded rat ovary using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0004.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded mouse brain using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0001.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded mouse kidney using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0002.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded rat brain using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0003.jpg)
![Western Blot: KAL1 Antibody [NBP3-03937] - Analysis of extracts of various cell lines, using KAL1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/KAL1-Antibody-Western-Blot-NBP3-03937-img0006.jpg "Western Blot: KAL1 Antibody [NBP3-03937] - Analysis of extracts of various cell lines, using KAL1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/ Immunofluorescence: KAL1 Antibody - Azide and BSA Free [NBP3-03937] - Immunofluorescence analysis of paraffin-embedded rat brain using KAL1 Rabbit pAb (A16389) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03937_rabbit-polyclonal-kal1-antibody-52202519294569.jpg)
![Immunocytochemistry/ Immunofluorescence: KAL1 Antibody - Azide and BSA Free [NBP3-03937] - Immunofluorescence analysis of paraffin-embedded mouse brain using KAL1 Rabbit pAb (A16389) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03937_rabbit-polyclonal-kal1-antibody-52202519302960.jpg)
![Immunocytochemistry/ Immunofluorescence: KAL1 Antibody - Azide and BSA Free [NBP3-03937] - Immunofluorescence analysis of paraffin-embedded mouse brain using KAL1 Rabbit pAb (A16389) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03937_rabbit-polyclonal-kal1-antibody-52202519313487.jpg)
![Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.](http://images.novusbio.com/fullsize/nbp3-02943_rabbit-huc-pab-52202516321480.jpg "Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.")
![Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Immunohistochemistry of paraffin-embedded mouse brain using HuC antibody (NBP3-02943) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HuC-Antibody-Immunohistochemistry-Paraffin-NBP3-02943-img0006.jpg)
![Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Paraffin-embedded rat brain using HuC antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/HuC-Antibody-Immunohistochemistry-Paraffin-NBP3-02943-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: HuC Antibody - Azide and BSA Free [HuC] - Immunofluorescence analysis of paraffin-embedded mouse brain using HuC Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02943_rabbit-huc-pab-52202516321493.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0002.jpg "Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Immunofluorescence analysis of Hep G2 cells using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Immunocytochemistry-Immunofluorescence-NBP3-15454-img0001.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of paraffin-embedded Mouse kidney using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-522025171899.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of Hep G2 cells using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-52202517192951.jpg)
![Western Blot: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis using GRM7 mAb against human GRM7 (AA: 454-590) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/mGluR7-Antibody-7G8D3-Western-Blot-NBP2-61856-img0005.jpg "Western Blot: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis using GRM7 mAb against human GRM7 (AA: 454-590) recombinant protein. (Expected MW is 41.7 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis of SMMC-7721 cells using GRM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/mGluR7-Antibody-7G8D3-Immunocytochemistry-Immunofluorescence-NBP2-61856-img0004.jpg)
![Western Blot: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis using GRM7 mAb against HEK293 (1) and GRM7 (AA: 454-590)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR7-Antibody-7G8D3-Western-Blot-NBP2-61856-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis of Hela cells using GRM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/mGluR7-Antibody-7G8D3-Immunocytochemistry-Immunofluorescence-NBP2-61856-img0003.jpg)
![ELISA: mGluR7 Antibody (7G8D3) [NBP2-61856] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR7-Antibody-7G8D3-ELISA-NBP2-61856-img0001.jpg)
![ELISA: mGluR7 Antibody (7G8D3) [NBP2-61856] - Analysis of SH-SY5Y cells using GRM7 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR7-Antibody-7G8D3-ELISA-NBP2-61856-img0002.jpg)
![Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Western-Blot-NBP2-42190-img0003.jpg "Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Lgi1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Lgi1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0005.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Western Blot: TBR1 Antibody [NBP2-15133] - A: Neuro2A B: GL261 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TBR1-Antibody-Western-Blot-NBP2-15133-img0002.jpg "Western Blot: TBR1 Antibody [NBP2-15133] - A: Neuro2A B: GL261 7.5% SDS PAGE diluted at 1:1000")
![Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-15133] - Paraffin-embedded mouse brain. TBR1 antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-15133-img0003.jpg)
![TBR1 antibody [N2C1], Internal detects TBR1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TBR1 stained by TBR1 antibody [N2C1], Internal (NBP2-15133) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15133_rabbit-polyclonal-tbr1-antibody-immunocytochemistry-immunofluorescence-2611202518402216.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody [NBP2-24617] - Formalin-fixed, paraffin-embedded human cervix tissue stained with Neuropilin-1 antibody [NBP2-24617] at 5 ug/ml.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24617-img0001.jpg "Immunohistochemistry-Paraffin: Neuropilin-1 Antibody [NBP2-24617] - Formalin-fixed, paraffin-embedded human cervix tissue stained with Neuropilin-1 antibody [NBP2-24617] at 5 ug/ml.")
![Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free](http://images.novusbio.com/fullsize/LIM1-Antibody---BSA-Free-Immunohistochemistry-NB110-12933-img0001.jpg "Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free")
![Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Staining of human lymph node.](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-62728-img0007.jpg "Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Staining of human lymph node.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Analysis in human cerebral cortex and kidney tissues using Anti-TBR1 antibody. Corresponding TBR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-62728-img0001.jpg)
![Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-62728-img0002.jpg)
![Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-62728-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Staining of human cerebral cortex, kidney, liver and lymph node using Anti-TBR1 antibody NBP2-62728 (A) shows similar protein distribution across tissues to independent antibody NBP2-62729 (B).](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-62728-img0005.jpg)
![Immunohistochemistry-Paraffin: TBR1 Antibody [NBP2-62728] - Staining of human liver.](http://images.novusbio.com/fullsize/TBR1-Antibody-Immunohistochemistry-Paraffin-NBP2-62728-img0006.jpg)