Neuroscience reagents: Novus Biologicals

NB300-326

GAPDH Antibody

(1 Review)

(3 Publications)

0.1 ml

NB300-326

Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.

Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.

Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-28635

Dtk/TYRO3 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-28635

Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Intracellular): Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-28635AF350
Alexa Fluor 405	NBP1-28635AF405
Alexa Fluor 488	NBP1-28635AF488
Alexa Fluor 532	NBP1-28635AF532
Alexa Fluor 594	NBP1-28635AF594
Alexa Fluor 647	NBP1-28635AF647
Alexa Fluor 700	NBP1-28635AF700
Alexa Fluor 750	NBP1-28635AF750
Allophycocyanin	NBP1-28635APC
Allophycocyanin/Cy7	NBP1-28635APCCY7
Biotin	NBP1-28635B
CoraFluor 1	NBP1-28635CL1
DyLight 350	NBP1-28635UV
DyLight 405	NBP1-28635V
DyLight 488	NBP1-28635G
DyLight 550	NBP1-28635R
DyLight 594	NBP1-28635DL594
DyLight 650	NBP1-28635C
DyLight 680	NBP1-28635FR
DyLight 755	NBP1-28635IR
FITC	NBP1-28635F
HRP	NBP1-28635H
Janelia Fluor 525	NBP1-28635JF525
Janelia Fluor 549	NBP1-28635JF549
Janelia Fluor 646	NBP1-28635JF646
Janelia Fluor 669	NBP1-28635JF669
PE	NBP1-28635PE
PE/Atto594	NBP1-28635PEATT594
PE/Cy5.5	NBP1-28635PECY55
PE/Cy7	NBP1-28635PECY7
PerCP	NBP1-28635PCP
mFluor Violet 450 SE	NBP1-28635MFV450
mFluor Violet 500 SE	NBP1-28635MFV500
mFluor Violet 610 SE	NBP1-28635MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-28635SS

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NBP2-29455

CD34 Antibody (ICO-115)

(1 Review)

(8 Publications)

0.1 mg

NBP2-29455

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-114A

Alt Catalog# ABX-114A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33076AF350
Alexa Fluor 405	NBP2-33076AF405
Alexa Fluor 488	NBP2-33076AF488
Alexa Fluor 532	NBP2-33076AF532
Alexa Fluor 594	NBP2-33076AF594
Alexa Fluor 647	NBP2-33076AF647
Alexa Fluor 700	NBP2-33076AF700
Alexa Fluor 750	NBP2-33076AF750
Allophycocyanin	NBP2-33076APC
Allophycocyanin/Cy7	NBP2-33076APCCY7
Biotin	NBP2-33076B
CoraFluor 1	NBP2-33076CL1
DyLight 350	NBP2-33076UV
DyLight 405	NBP2-33076V
DyLight 488	NBP2-33076G
DyLight 550	NBP2-33076R
DyLight 594	NBP2-33076DL594
DyLight 650	NBP2-33076C
DyLight 680	NBP2-33076FR
DyLight 755	NBP2-33076IR
FITC	NBP2-33076F
HRP	NBP2-33076H
Janelia Fluor 525	NBP2-33076JF525
Janelia Fluor 549	NBP2-33076JF549
Janelia Fluor 646	NBP2-33076JF646
Janelia Fluor 669	NBP2-33076JF669
PE	NBP2-33076PE
PE/Atto594	NBP2-33076PEATT594
PE/Cy5.5	NBP2-33076PECY55
PE/Cy7	NBP2-33076PECY7
PerCP	NBP2-33076PCP
mFluor Violet 450 SE	NBP2-33076MFV450
mFluor Violet 500 SE	NBP2-33076MFV500
mFluor Violet 610 SE	NBP2-33076MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33076

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NB300-186

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

(2 Publications)

0.1 ml

NB300-186

$Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.$

Simple Western: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Simple Western lane view shows a specific band for SynCAM in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND35 male (top) and female (middle) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group. Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05, **p<0.01, ***p<0.001 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-186SS

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NBP2-43765

Calnexin Antibody (1563) - Azide and BSA Free

(5 Publications)

0.1 ml

NBP2-43765

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765] - Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis.Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calnexin stained by Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:500.Blue: Fluoroshield with DAPI .

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:6000.

Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Calnexin stained by Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:500.Red: Calnexin, stained by Calnexin antibody [C3], C-term diluted at 1:500.Blue: Hoechst 33342 staining.

AKT acts down-stream of mTOR signaling. To examine if Akt is upstream or downstream of mTOR signaling (A), osteoclast precursors were treated with RANKL (50 ng/ml) for 2–3 days with and without pyruvate (1 mM). (B) The levels of phosphorylated (Ser473) and total Akt in control and pyruvate-supplemented cultures, alpha -tubulin was used as a control for protein loading. (C) Cells cultured without pyruvate were exposed to Akt inhibitor (AI, 5 μM) or rapamycin (1, 10, or 100 nM), and mTOR phosphorylation, total mTOR levels and phosphorylation of p70S6K was assessed, calnexin was used as a protein loading control. The numbers above the blots indicate phospho-S6K levels relative to calnexin. (D) The effect of Akt inhibitor or rapamycin on Akt phosphorylation. The numbers above the blots indicate phospho-Akt levels relative to total Akt. To be noted, all the lanes were parts of the same gel, however, the lane order was changed, resulting in discontinuity. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28573133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-19711

Pax6 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP2-19711

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody (NBP2-19711) diluted at 1:250.

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:200.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Samples: Paraffin-Embedded mouse retina.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 um.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-15052

OCT4 Antibody (486) - Azide and BSA Free

(1 Review)

0.1 ml

NBP2-15052

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.

Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.

<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.A. 30 ug human ESC whole cell lysate/extract10% SDS-PAGE (NBP2-15052) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rb, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rb, Pm, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-77679

IGF-I R/IGF1R Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-77679

Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77679] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77679). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77679AF350
Alexa Fluor 405	NBP1-77679AF405
Alexa Fluor 488	NBP1-77679AF488
Alexa Fluor 532	NBP1-77679AF532
Alexa Fluor 594	NBP1-77679AF594
Alexa Fluor 647	NBP1-77679AF647
Alexa Fluor 700	NBP1-77679AF700
Alexa Fluor 750	NBP1-77679AF750
Allophycocyanin	NBP1-77679APC
Biotin	NBP1-77679B
CoraFluor 1	NBP1-77679CL1
DyLight 350	NBP1-77679UV
DyLight 405	NBP1-77679V
DyLight 488	NBP1-77679G
DyLight 550	NBP1-77679R
DyLight 594	NBP1-77679DL594
DyLight 650	NBP1-77679C
DyLight 680	NBP1-77679FR
DyLight 755	NBP1-77679IR
FITC	NBP1-77679F
HRP	NBP1-77679H
Janelia Fluor 525	NBP1-77679JF525
Janelia Fluor 549	NBP1-77679JF549
Janelia Fluor 646	NBP1-77679JF646
Janelia Fluor 669	NBP1-77679JF669
PE	NBP1-77679PE
PerCP	NBP1-77679PCP
mFluor Violet 450 SE	NBP1-77679MFV450
mFluor Violet 500 SE	NBP1-77679MFV500
mFluor Violet 610 SE	NBP1-77679MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77679SS

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NB100-2381

VEGF Antibody - BSA Free

(7 Publications)

0.1 ml

NB100-2381

HIF-1 alpha protein and HIF-1 alpha target genes are elevated in SCs following retrovirus administration. Expression of mRNA for VP16, HIF-1 alpha , and beta -actin in transfected SCs was confirmed by PCR in VP16 and VP16-HIF SCs (A). Protein expression of VP16, HIF-1 alpha , HIF-2 alpha , VEGF, enolase, and protein loading controls (GFP or beta -actin) in transfected SCs was assessed by western blotting (B). Protein expression was normalized to the loading control. Relative intensity of VP16 expression is shown in C, and the fold change in protein expression relative to control SCs is shown for HIF-1 alpha (D) HIF-2 alpha (E), VEGF (F), and enolase (G). Values are as follows: VP16 (control: 0.0 +/- 0.0; VP16: 0.40 +/- 0.01; VP16-HIF: 0.41 +/- 0.00); HIF-1 alpha (control: 1.0 +/- 0.04; VP16: 1.0 +/- 0.04; VP16-HIF: 5.9 +/- 0.01); HIF-2 alpha (control: 1.0 +/- 0.01; VP16: 1.0 +/- 0.01; VP16-HIF: 1.0 +/- 0.02); VEGF (control: 1.0 +/- 0.01; VP16: 1.02 +/- 0.02; VP16-HIF: 2.2 +/- 0.01); enolase (control: 1.0 +/- 0.01; VP16: 1.0 +/- 0.04; VP16-HIF: 1.7 +/- 0.00); n = 3/group. Mean +/- SEM, *p < 0.0001. Figure Contributions: Veena Kandaswamy generated the VP16-HIF cells. Veena Kandaswamy performed the PCRs. Ying Dai performed the western blottings. Ying Dai and Caitlin Hill analyzed the data. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31488552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch, Eq, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Eq, Gp

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2381AF350
Alexa Fluor 405	NB100-2381AF405
Alexa Fluor 488	NB100-2381AF488
Alexa Fluor 532	NB100-2381AF532
Alexa Fluor 594	NB100-2381AF594
Alexa Fluor 647	NB100-2381AF647
Alexa Fluor 700	NB100-2381AF700
Alexa Fluor 750	NB100-2381AF750
Allophycocyanin	NB100-2381APC
Biotin	NB100-2381B
CoraFluor 1	NB100-2381CL1
DyLight 350	NB100-2381UV
DyLight 405	NB100-2381V
DyLight 488	NB100-2381G
DyLight 550	NB100-2381R
DyLight 594	NB100-2381DL594
DyLight 650	NB100-2381C
DyLight 680	NB100-2381FR
DyLight 755	NB100-2381IR
FITC	NB100-2381F
HRP	NB100-2381H
Janelia Fluor 525	NB100-2381JF525
Janelia Fluor 549	NB100-2381JF549
Janelia Fluor 646	NB100-2381JF646
Janelia Fluor 669	NB100-2381JF669
PE	NB100-2381PE
PerCP	NB100-2381PCP
mFluor Violet 450 SE	NB100-2381MFV450
mFluor Violet 500 SE	NB100-2381MFV500
mFluor Violet 610 SE	NB100-2381MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2381SS

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NBP2-22139

Sonic Hedgehog/Shh Antibody - BSA Free

(5 Publications)

0.1 ml

NBP2-22139

Immunohistochemistry: Sonic Hedgehog Antibody [NBP2-22139] - IHC analysis of Sonic Hedgehog in mouse kidney.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22139AF350
Alexa Fluor 405	NBP2-22139AF405
Alexa Fluor 488	NBP2-22139AF488
Alexa Fluor 532	NBP2-22139AF532
Alexa Fluor 594	NBP2-22139AF594
Alexa Fluor 647	NBP2-22139AF647
Alexa Fluor 700	NBP2-22139AF700
Alexa Fluor 750	NBP2-22139AF750
Allophycocyanin	NBP2-22139APC
Biotin	NBP2-22139B
CoraFluor 1	NBP2-22139CL1
DyLight 350	NBP2-22139UV
DyLight 405	NBP2-22139V
DyLight 488	NBP2-22139G
DyLight 550	NBP2-22139R
DyLight 594	NBP2-22139DL594
DyLight 650	NBP2-22139C
DyLight 680	NBP2-22139FR
DyLight 755	NBP2-22139IR
FITC	NBP2-22139F
HRP	NBP2-22139H
Janelia Fluor 525	NBP2-22139JF525
Janelia Fluor 549	NBP2-22139JF549
Janelia Fluor 646	NBP2-22139JF646
Janelia Fluor 669	NBP2-22139JF669
PE	NBP2-22139PE
PerCP	NBP2-22139PCP
mFluor Violet 450 SE	NBP2-22139MFV450
mFluor Violet 500 SE	NBP2-22139MFV500
mFluor Violet 610 SE	NBP2-22139MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22139SS

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NBP1-85607

NDUFB5 Antibody - BSA Free

(1 Review)

0.1 ml

NBP1-85607

Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP1-85607] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Frozen: NDUFB5 Antibody [NBP1-85607] - PFA-fixed cryosections (14 um) of adult mouse brain.100x confocal imaging. Mitochondria are labeled. Antigen retrieval was performed in boiling 10mM citric acid (pH6) for 15 min, followed by block in 5% goat serum (PBS/ 0.3% triton) and incubated in primary ab (1:100) overnight at 4C in goat block. Developed with goat-anti-rabbit Alexa 488. Image from verified customer review.

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-85607] - Staining of human heart muscle shows strong positivity in mitochondria in cardiomyocytes.

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-85607] - Staining of human pancreas shows weak positivity in mitochondria in exocrine glandular cells.

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-85607] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85607-25ul

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NBP1-87781

Myelin PLP Antibody - BSA Free

(1 Review)

(6 Publications)

0.1 ml

NBP1-87781

Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87781-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

GAPDH Antibody

Dtk/TYRO3 Antibody - BSA Free

CD34 Antibody (ICO-115)

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

Calnexin Antibody (1563) - Azide and BSA Free

Pax6 Antibody - BSA Free

OCT4 Antibody (486) - Azide and BSA Free

IGF-I R/IGF1R Antibody - BSA Free

VEGF Antibody - BSA Free

Sonic Hedgehog/Shh Antibody - BSA Free

NDUFB5 Antibody - BSA Free

Myelin PLP Antibody - BSA Free