Neuroscience reagents: Novus Biologicals

NBP1-28635

Dtk/TYRO3 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-28635

Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Intracellular): Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-28635AF350
Alexa Fluor 405	NBP1-28635AF405
Alexa Fluor 488	NBP1-28635AF488
Alexa Fluor 532	NBP1-28635AF532
Alexa Fluor 594	NBP1-28635AF594
Alexa Fluor 647	NBP1-28635AF647
Alexa Fluor 700	NBP1-28635AF700
Alexa Fluor 750	NBP1-28635AF750
Allophycocyanin	NBP1-28635APC
Allophycocyanin/Cy7	NBP1-28635APCCY7
Biotin	NBP1-28635B
CoraFluor 1	NBP1-28635CL1
DyLight 350	NBP1-28635UV
DyLight 405	NBP1-28635V
DyLight 488	NBP1-28635G
DyLight 550	NBP1-28635R
DyLight 594	NBP1-28635DL594
DyLight 650	NBP1-28635C
DyLight 680	NBP1-28635FR
DyLight 755	NBP1-28635IR
FITC	NBP1-28635F
HRP	NBP1-28635H
Janelia Fluor 525	NBP1-28635JF525
Janelia Fluor 549	NBP1-28635JF549
Janelia Fluor 646	NBP1-28635JF646
Janelia Fluor 669	NBP1-28635JF669
PE	NBP1-28635PE
PE/Atto594	NBP1-28635PEATT594
PE/Cy5.5	NBP1-28635PECY55
PE/Cy7	NBP1-28635PECY7
PerCP	NBP1-28635PCP
mFluor Violet 450 SE	NBP1-28635MFV450
mFluor Violet 500 SE	NBP1-28635MFV500
mFluor Violet 610 SE	NBP1-28635MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-28635SS

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NBP2-19711

Pax6 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP2-19711

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody (NBP2-19711) diluted at 1:250.

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:200.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Samples: Paraffin-Embedded mouse retina.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 um.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-15052

OCT4 Antibody (486) - Azide and BSA Free

(1 Review)

0.1 ml

NBP2-15052

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.

Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.

<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.A. 30 ug human ESC whole cell lysate/extract10% SDS-PAGE (NBP2-15052) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rb, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rb, Pm, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB300-186

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

(2 Publications)

0.1 ml

NB300-186

$Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.$

Simple Western: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Simple Western lane view shows a specific band for SynCAM in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND35 male (top) and female (middle) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group. Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05, **p<0.01, ***p<0.001 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-186SS

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NBP2-57308

MCT8/SLC16A2 Antibody - BSA Free

(6 Publications)

100 ul

NBP2-57308

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cerebral cortex shows moderate positivity in neuropil.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human liver shows moderate to strong membranous positivity in hepatocytes.

Expression of MCT8 and OATP1C1 in the cerebral barriers of the human and macaque motor cortex. Representative brightfield (A,E,I,M) and fluorescence confocal microscope (B–D,F–H,J–L,N–T) photomicrographs showing immunostaining for MCT8 (A–H,Q–T) and OATP1C1 (I–P) in blood vessels of human and macaque motor cortex. (A,E) show strong MCT8 staining in the endothelial layer of small vessels and capillaries (white arrowheads) in humans and macaques, respectively. (B–D,F–H) show colocalization of MCT8 (green) with the endothelial markers UEA-I (red) and ENG (red). (I,M) show weak OATP1C1 staining in small vessels (white arrowheads) and venules (black arrowheads). The red arrowhead in M points to a glial cell positive for OATP1C1 with processes surrounding the capillary. (J–L,N–P) show colocalization of OATP1C1 (green) with UEA-I (red) and endoglin (red) in a few blood vessels of the human and macaque motor cortex, respectively. White arrowheads point to vessels. In both human and macaque brain tissues, MCT8-immunopositive capillaries are much more abundant than OATP1C1-immunopositive capillaries, which can only be seen occasionally. Moreover, (Q–T) show MCT8-expressing pericytes (white arrowheads) in the human motor cortex that in addition to express MCT8 (green), also show the pericyte biomarker PDGFR-beta (pink), but not the endothelial marker UEA-I (red). Counterstaining with DAPI (blue) shows nuclei of all cells. ENG: endoglin, UEA-I: Ulex Europaeus Agglutinin-I. PDGFR-beta : platelet-derived growth factor receptor beta. Scale bar = 100 μm (A,E,I,M), 25 μm (B–D), 50 μm (J–L), 120 μm (F–H,N–P), and 12 μm (Q–T). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of MCT8 in pyramidal neurons of the human and macaque motor cortex. Representative brightfield photomicrographs show immunostaining for MCT8 in layers III (A), V (B) and VI (C) of the human motor cortex, and layers II (G), III (H), and V (I) of the macaque motor cortex. White arrowheads point to pyramidal neurons with immunopositive signal in the soma, apical and basal dendrites. Black arrowheads point to smaller cells with the morphology of interneurons. (D–F) (human) and (J–L) (macaque) show confocal microscope images from double-stained sections for MCT8 (green) and the pyramidal neuron markers SMI-32 (red) for 200 kDa neurofilament protein or neurogranin/RC3 (red), respectively. White arrowheads point to pyramidal neurons. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans, the MCT8 signal is located mainly in the pyramidal cell membrane, while in macaques it is located in the membrane and the cytoplasm. Scale bar = 50 μm (A), 120 μm (B,I), 25 μm (C–F), 60 μm (G), 62.5 μm (H), and 43.5 μm (J–L). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of MCT8 and OATP1C1 in human and macaque motor cortex astrocytes. Representative brightfield (A–E,I) photomicrographs of sections immunostained for MCT8 (A,C) and OATP1C1 (B,D,E,I) in cortical layer I (A–D) and subjacent white matter (E,I). Confocal microscope images of human (F–H) and macaque (J–O) sections double-labeled for OATP1C1 (green) and GFAP (red). Counterstaining with DAPI (blue) shows nuclei of all cells. Note that there is full colocalization of both markers. White and red arrowheads point to astrocytes. Blue arrowheads point to a blood vessel. GFAP: glial fibrillary acidic protein. Scale bar = 50 μm (A–D,J–L), 110 μm (E,I), 25 μm (F–H), and 78 μm (M–O). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57308-25ul

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NBP1-87781

Myelin PLP Antibody - BSA Free

(1 Review)

(6 Publications)

0.1 ml

NBP1-87781

Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87781-25ul

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NBP1-85814

Vimentin Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-85814

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85814-25ul

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NBP3-11863

TET1 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP3-11863

Immunohistochemistry-Paraffin: TET1 Antibody [NBP3-11863] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TET1 antibody (NBP3-11863). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on NIH3T3 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - HeLa cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - NIH3T3 cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on A431 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11863AF350
Alexa Fluor 405	NBP3-11863AF405
Alexa Fluor 488	NBP3-11863AF488
Alexa Fluor 532	NBP3-11863AF532
Alexa Fluor 594	NBP3-11863AF594
Alexa Fluor 647	NBP3-11863AF647
Alexa Fluor 700	NBP3-11863AF700
Alexa Fluor 750	NBP3-11863AF750
Allophycocyanin	NBP3-11863APC
Biotin	NBP3-11863B
CoraFluor 1	NBP3-11863CL1
DyLight 350	NBP3-11863UV
DyLight 405	NBP3-11863V
DyLight 488	NBP3-11863G
DyLight 550	NBP3-11863R
DyLight 594	NBP3-11863DL594
DyLight 650	NBP3-11863C
DyLight 680	NBP3-11863FR
DyLight 755	NBP3-11863IR
FITC	NBP3-11863F
HRP	NBP3-11863H
Janelia Fluor 525	NBP3-11863JF525
Janelia Fluor 549	NBP3-11863JF549
Janelia Fluor 646	NBP3-11863JF646
Janelia Fluor 669	NBP3-11863JF669
PE	NBP3-11863PE
PerCP	NBP3-11863PCP
mFluor Violet 450 SE	NBP3-11863MFV450
mFluor Violet 500 SE	NBP3-11863MFV500
mFluor Violet 610 SE	NBP3-11863MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-11863SS

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NB100-938

LRRK2 Antibody Pack

1 Pack

NB100-938

Immunohistochemistry: LRRK2 Antibody Pack [NB100-938] - Staining of neurons and glia using NB110-55289 at 2.5ug/ml. Mouse brain, 40x.

Western Blot: LRRK2 Antibody Pack [NB100-938] - Detection of murine LRRK2 in mouse brain lysate using NB110-55289.

Reactivity:

Hu, Mu, Bv, Ce, I, Pl, Hu(-)

Species Glossary

Reactivity: Hu, Mu, Bv, Ce, I, Pl, Hu(-)

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit

Host: Rabbit

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NBP1-47977

VMAT2 Antibody (OTI9E11)

(1 Review)

(1 Publication)

0.1 ml

NBP1-47977

Immunohistochemistry-Paraffin: VMAT2 Antibody (9E11) [NBP1-47977] - Staining of paraffin-embedded Human pancreas tissue using anti-VMAT2 mouse monoclonal antibody.

Flow Cytometry: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VMAT2 antibody, and then analyzed by flow cytometry.

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMAT2.

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti SLC18A2 monoclonal antibody.(1:200).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74850AF350
Alexa Fluor 405	NBP2-74850AF405
Alexa Fluor 488	NBP2-74850AF488
Alexa Fluor 532	NBP2-74850AF532
Alexa Fluor 594	NBP2-74850AF594
Alexa Fluor 647	NBP2-74850AF647
Alexa Fluor 700	NBP2-74850AF700
Alexa Fluor 750	NBP2-74850AF750
Allophycocyanin	NBP2-74850APC
Biotin	NBP2-74850B
CoraFluor 1	NBP2-74850CL1
DyLight 350	NBP2-74850UV
DyLight 405	NBP2-74850V
DyLight 488	NBP2-74850G
DyLight 550	NBP2-74850R
DyLight 594	NBP2-74850DL594
DyLight 650	NBP2-74850C
DyLight 680	NBP2-74850FR
DyLight 755	NBP2-74850IR
FITC	NBP2-74850F
HRP	NBP2-74850H
Janelia Fluor 525	NBP2-74850JF525
Janelia Fluor 549	NBP2-74850JF549
Janelia Fluor 646	NBP2-74850JF646
Janelia Fluor 669	NBP2-74850JF669
PE	NBP2-74850PE
PerCP	NBP2-74850PCP
mFluor Violet 450 SE	NBP2-74850MFV450
mFluor Violet 500 SE	NBP2-74850MFV500
mFluor Violet 610 SE	NBP2-74850MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74850

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NBP1-75522

Orai1 Antibody (3F6H5) - BSA Free

(6 Publications)

0.1 mg

NBP1-75522

Immunohistochemistry: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 5 u/mL.

Immunohistochemistry: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-75522AF350
Alexa Fluor 405	NBP1-75522AF405
Alexa Fluor 488	NBP1-75522AF488
Alexa Fluor 532	NBP1-75522AF532
Alexa Fluor 594	NBP1-75522AF594
Alexa Fluor 647	NBP1-75522AF647
Alexa Fluor 700	NBP1-75522AF700
Alexa Fluor 750	NBP1-75522AF750
Allophycocyanin	NBP1-75522APC
Biotin	NBP1-75522B
CoraFluor 1	NBP1-75522CL1
DyLight 350	NBP1-75522UV
DyLight 405	NBP1-75522V
DyLight 488	NBP1-75522G
DyLight 550	NBP1-75522R
DyLight 594	NBP1-75522DL594
DyLight 650	NBP1-75522C
DyLight 680	NBP1-75522FR
DyLight 755	NBP1-75522IR
FITC	NBP1-75522F
HRP	NBP1-75522H
Janelia Fluor 525	NBP1-75522JF525
Janelia Fluor 549	NBP1-75522JF549
Janelia Fluor 646	NBP1-75522JF646
Janelia Fluor 669	NBP1-75522JF669
PE	NBP1-75522PE
PerCP	NBP1-75522PCP
mFluor Violet 450 SE	NBP1-75522MFV450
mFluor Violet 500 SE	NBP1-75522MFV500
mFluor Violet 610 SE	NBP1-75522MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-75522-0.025mg

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NBP2-01926

LIM1 Antibody (OTI2D5)

(4 Publications)

0.1 ml

NBP2-01926

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.

Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.

Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71134AF350
Alexa Fluor 405	NBP2-71134AF405
Alexa Fluor 488	NBP2-71134AF488
Alexa Fluor 532	NBP2-71134AF532
Alexa Fluor 594	NBP2-71134AF594
Alexa Fluor 647	NBP2-71134AF647
Alexa Fluor 700	NBP2-71134AF700
Alexa Fluor 750	NBP2-71134AF750
Allophycocyanin	NBP2-71134APC
Biotin	NBP2-71134B
CoraFluor 1	NBP2-71134CL1
DyLight 350	NBP2-71134UV
DyLight 405	NBP2-71134V
DyLight 488	NBP2-71134G
DyLight 550	NBP2-71134R
DyLight 594	NBP2-71134DL594
DyLight 650	NBP2-71134C
DyLight 680	NBP2-71134FR
DyLight 755	NBP2-71134IR
FITC	NBP2-71134F
HRP	NBP2-71134H
Janelia Fluor 525	NBP2-71134JF525
Janelia Fluor 549	NBP2-71134JF549
Janelia Fluor 646	NBP2-71134JF646
Janelia Fluor 669	NBP2-71134JF669
PE	NBP2-71134PE
PerCP	NBP2-71134PCP
mFluor Violet 450 SE	NBP2-71134MFV450
mFluor Violet 500 SE	NBP2-71134MFV500
mFluor Violet 610 SE	NBP2-71134MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71134

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Dtk/TYRO3 Antibody - BSA Free

Pax6 Antibody - BSA Free

OCT4 Antibody (486) - Azide and BSA Free

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

MCT8/SLC16A2 Antibody - BSA Free

Myelin PLP Antibody - BSA Free

Vimentin Antibody - BSA Free

TET1 Antibody - BSA Free

LRRK2 Antibody Pack

VMAT2 Antibody (OTI9E11)

Orai1 Antibody (3F6H5) - BSA Free

LIM1 Antibody (OTI2D5)