| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34808-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-154SS | |||
| Azide and BSA Free | NBP2-80871 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54544-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46852SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08431 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87103-25ul | |||
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0002.jpg "Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse brain tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0001.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 µg/Lane)Lane 2: Human liver tissue lysate (20 µg/Lane)Lane 3: Mouse heart tissue lysate (20 µg/Lane)Lane 4: Rat heart tissue lysate (20 µg/Lane)Lane 5: Mouse kidney tissue lysate (20 µg/Lane)Lane 6: Rat kidney tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-28620249199..jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/1,000 dilution.Lane 1: A431 cell lysate (no heat) (15 µg/Lane)Lane 2: Mouse kidney tissue lysate (no heat) (20 µg/Lane)Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)Lane 4: Rat kidney tissue lysate (no heat) (20 µg/Lane)Lane 5: Zebrafish tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-286202492022..jpg)
![Flow Cytometry-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of A431 cells labeling beta 2 Adrenergic Receptor.Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-flow-cytometry-28620249222..jpg)
![ICC/IF-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of A431 cells labeling beta 2 Adrenergic Receptor with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-immunocytochemistry-immunofluorescence-286202492524..jpg)
![Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Western-Blot-NBP2-67410-img0005.jpg "Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410] - ICC staining of BDNF in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunocytochemistry-Immunofluorescence-NBP2-67410-img0006.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0001.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse brain tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0002.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0003.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Western-Blot-NBP2-32896-img0003.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Immunofluorescence of skin innervation using anti-PGP9.5. Antibody at dilution 1:500. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Immunocytochemistry-Immunofluorescence-NBP2-32896-img0002.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27b-Immunohistochemistry-Paraffin-NB300-154-img0002.jpg "Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.")
![Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Western-Blot-NBP2-53358-img0004.jpg "Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (15B8) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunocytochemistry-Immunofluorescence-NBP2-53358-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunohistochemistry-Paraffin-NBP2-53358-img0001.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0011.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46852-img0006.jpg "Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46852] - Immunohistochemical analysis of RCAN1 in human fetal brain.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46852-img0003.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46852] - Analysis of RCAN1 in human fetal brain.](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46852-img0004.jpg)
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0011.jpg "Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on HeLa cell. Antibody concentration 35 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunocytochemistry-Immunofluorescence-H00005058-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunohistochemistry-Paraffin-H00005058-M02-img0008.jpg)
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0001.jpg)
![Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunoprecipitation-H00005058-M02-img0009.jpg)
![Sandwich ELISA: PAK1 Antibody (4D1) [H00005058-M02] - Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Sandwich-ELISA-H00005058-M02-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S GFAP antibody (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).](http://images.novusbio.com/fullsize/GFAP-Antibody-ASTRO-1974R-Immunocytochemistry-Immunofluorescence-NBP3-07877-img0003.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).](http://images.novusbio.com/fullsize/GFAP-Antibody-ASTRO-1974R-Immunohistochemistry-Paraffin-NBP3-07877-img0002.jpg)
![Flow Cytometry: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/GFAP-Antibody-ASTRO-1974R-Flow-Cytometry-NBP3-07877-img0001.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0016.jpg "Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0015.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0013.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0014.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422625.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374983.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374923.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422673.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249393552.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-212024941465.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249415991.jpg)