| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91920-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87915-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43662-25ul | |||
| Azide and BSA Free | NBP3-44322 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44492 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43810 | |||
| BSA Free | NBP2-46636-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-50056-20ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05204-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34808-0.1mg | |||
![Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GAT3-Antibody-Western-Blot-NBP1-91920-img0006.jpg "Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebellum shows strong positivity in processes in cells in molecular layer.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0007.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0008.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87915-img0006.jpg "Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Analysis in human testis and liver tissues. Corresponding ENAH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human endometrium, liver, pancreas and tonsil using Anti-AGXT antibody (A) NBP1-87915 shows similar protein distribution across tissues to independent antibody NBP1-87914 (B).](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0021.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0023.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0018.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0019.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87915-img0020.jpg)
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GAPDH Antibody (CL3266) [NBP2-59779] - Staining of A549 cells using the Anti-GAPDH monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunocytochemistry-Immunofluorescence-NBP2-59779-img0013.jpg)
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0008.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human kidney shows cytoplasmic and nuclear immunoreactivity in renal tubules and glomerular cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human fallopian tube shows cytoplasmic and nuclear positivity in the epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human liver shows cytoplasmic and nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0004.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human pancreas shows nuclear and cytoplasmic immunoreactivity in both endocrine and exocrine cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0005.jpg)
![Western Blot: CNPase Antibody (2872) [NBP2-46636] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Western-Blot-NBP2-46636-img0009.jpg "Western Blot: CNPase Antibody (2872) [NBP2-46636] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2872) [NBP2-46636] - CNPase Antibody (2872) [NBP2-46636] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0007.jpg)
![Western Blot: CNPase Antibody (2872) [NBP2-46636] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Western-Blot-NBP2-46636-img0008.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of rat cerebral cortex shows moderate immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0001.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of mouse striatum shows strong positivity in oligodendrocytes and myelinated neural fibers.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0002.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2872) [NBP2-46636] - CNPase Antibody (2872) [NBP2-46636] - Staining of human cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0003.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of rat cerebral cortex shows strong immunoreactivity in a single oligodendrocyte.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0004.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of mouse cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0005.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2872) [NBP2-46636] - CNPase Antibody (2872) [NBP2-46636] - Staining of human cerebellum shows strong immunoreactivity in oligodendrocytes and myelinated neural processes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0006.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NB100-60944-img0011.jpg "Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-60944-img0013.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.02 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NB100-60944-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-60944-img0012.jpg)
![Western Blot: TRPM2 Antibody [NBP1-59618] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/TRPM2-Antibody-Western-Blot-NBP1-59618-img0006.jpg "Western Blot: TRPM2 Antibody [NBP1-59618] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-59618] - Human Heart Tissue, 10-20 ug/ml antibody concentration.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunohistochemistry-Paraffin-NBP1-59618-img0005.jpg)
![Western Blot: TRPM2 Antibody [NBP1-59618] - Human Fetal Brain Lysate, concentration 1ug/ml.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Western-Blot-NBP1-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-59618] - human LCL and mouse brains incubated for 5 minutes in CDP-Star for 5 minutes tissue.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunohistochemistry-Paraffin-NBP1-59618-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0014.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0002.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0003.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0005.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0006.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0008.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0009.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific OrangeTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0010.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) biotin.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0011.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of human peripheral blood with anti-CD16 (3G8) FITC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0012.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0013.jpg)
![Western Blot: MBP Antibody (7D2) [NBP1-05204] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05204, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord. Bands at 21.5kDa and 18.5kDa are the two larger transcripts from the MBP gene, showing that the epitope of this antibody depends on the sequence encoded by exon 2. Note that monoclonal NBP1-05203 binds all four rat MBP transcripts running at 21.5kDa, 18.5kDa, 17kDa and 14kDa, so that this antibody binds to the core sequence of human and rodent MBP.](http://images.novusbio.com/fullsize/MBP-Antibody-7D2-Western-Blot-NBP1-05204-img0002.jpg "Western Blot: MBP Antibody (7D2) [NBP1-05204] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05204, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord. Bands at 21.5kDa and 18.5kDa are the two larger transcripts from the MBP gene, showing that the epitope of this antibody depends on the sequence encoded by exon 2. Note that monoclonal NBP1-05203 binds all four rat MBP transcripts running at 21.5kDa, 18.5kDa, 17kDa and 14kDa, so that this antibody binds to the core sequence of human and rodent MBP.")
![Immunohistochemistry: MBP Antibody (7D2) [NBP1-05204] - Analysis rat brain hippocampal section stained with mouse mAb to myelin basic protein (MBP), NBP1-05204, dilution 1:5,000 in green, and costained with rabbit pAb to NF-M, RPCA-NF-M, dilution 1:2,000, in red. The MBP antibody stains myelin sheathes around axons, while the NF-M antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/MBP-Antibody-7D2-Immunocytochemistry-Immunofluorescence-NBP1-05204-img0001.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Western-Blot-NBP2-32896-img0003.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Immunofluorescence of skin innervation using anti-PGP9.5. Antibody at dilution 1:500. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Immunocytochemistry-Immunofluorescence-NBP2-32896-img0002.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB300-264-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB300-264-img0008.jpg)
![Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunoprecipitation-NB300-264-img0013.jpg)