Neuroscience reagents: Novus Biologicals

NBP1-92156

Myosin 5a Antibody - BSA Free

(10 Publications)

0.1 ml

NBP1-92156

Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human kIdney shows moderate memberanous positivity in cells in tubules.

Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebral cortex shows strong membranous positivity in neuropil.

Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human hippocampus shows strong cytoplasmic positivity in pyramidal cells.

Staining of human liver shows no positivity in hepatocytes as expected.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-MYO5A antibody. Corresponding MYO5A RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-92156-25ul

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NB120-13249

Bassoon Antibody (SAP7F407) - BSA Free

(1 Review)

(15 Publications)

0.05 mg

NB120-13249

Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-13249AF350
Alexa Fluor 532	NB120-13249AF532
Alexa Fluor 594	NB120-13249AF594
Alexa Fluor 750	NB120-13249AF750
Biotin	NB120-13249B
CoraFluor 1	NB120-13249CL1
DyLight 550	NB120-13249R
DyLight 594	NB120-13249DL594
DyLight 650	NB120-13249C
FITC	NB120-13249F
HRP	NB120-13249H
Janelia Fluor 525	NB120-13249JF525
Janelia Fluor 549	NB120-13249JF549
Janelia Fluor 646	NB120-13249JF646
Janelia Fluor 669	NB120-13249JF669
mFluor Violet 450 SE	NB120-13249MFV450
mFluor Violet 500 SE	NB120-13249MFV500
mFluor Violet 610 SE	NB120-13249MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB120-13249-0.2mg
BSA Free	NB120-13249-0.025mg

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NBP1-51841

Mitofusin 1 Antibody - BSA Free

(1 Review)

(10 Publications)

0.1 ml

NBP1-51841

Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.

Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-51841AF350
Alexa Fluor 405	NBP1-51841AF405
Alexa Fluor 488	NBP1-51841AF488
Alexa Fluor 532	NBP1-51841AF532
Alexa Fluor 594	NBP1-51841AF594
Alexa Fluor 647	NBP1-51841AF647
Alexa Fluor 700	NBP1-51841AF700
Alexa Fluor 750	NBP1-51841AF750
Allophycocyanin	NBP1-51841APC
Biotin	NBP1-51841B
CoraFluor 1	NBP1-51841CL1
DyLight 350	NBP1-51841UV
DyLight 405	NBP1-51841V
DyLight 488	NBP1-51841G
DyLight 550	NBP1-51841R
DyLight 594	NBP1-51841DL594
DyLight 650	NBP1-51841C
DyLight 680	NBP1-51841FR
DyLight 755	NBP1-51841IR
FITC	NBP1-51841F
HRP	NBP1-51841H
Janelia Fluor 525	NBP1-51841JF525
Janelia Fluor 549	NBP1-51841JF549
Janelia Fluor 646	NBP1-51841JF646
Janelia Fluor 669	NBP1-51841JF669
PE	NBP1-51841PE
PerCP	NBP1-51841PCP
mFluor Violet 450 SE	NBP1-51841MFV450
mFluor Violet 500 SE	NBP1-51841MFV500
mFluor Violet 610 SE	NBP1-51841MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-51841SS

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NB300-529

SERCA2 ATPase Antibody (IID8) - BSA Free

(8 Publications)

100uL

NB300-529

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Heart tissue.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Liver tissue.

Reactivity:

Hu, Mu, Rt, Po, Am, Bv, Ca, Gp, Ha, Pm, Rb, Sh, V-Vi

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Gp, Ha, Pm, Rb, Sh, V-Vi

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-529AF350
Alexa Fluor 405	NB300-529AF405
Alexa Fluor 488	NB300-529AF488
Alexa Fluor 532	NB300-529AF532
Alexa Fluor 594	NB300-529AF594
Alexa Fluor 647	NB300-529AF647
Alexa Fluor 700	NB300-529AF700
Alexa Fluor 750	NB300-529AF750
Allophycocyanin	NB300-529APC
Biotin	NB300-529B
CoraFluor 1	NB300-529CL1
DyLight 350	NB300-529UV
DyLight 405	NB300-529V
DyLight 488	NB300-529G
DyLight 550	NB300-529R
DyLight 594	NB300-529DL594
DyLight 650	NB300-529C
DyLight 680	NB300-529FR
DyLight 755	NB300-529IR
FITC	NB300-529F
HRP	NB300-529H
Janelia Fluor 525	NB300-529JF525
Janelia Fluor 549	NB300-529JF549
Janelia Fluor 646	NB300-529JF646
Janelia Fluor 669	NB300-529JF669
PE	NB300-529PE
mFluor Violet 450 SE	NB300-529MFV450
mFluor Violet 500 SE	NB300-529MFV500
mFluor Violet 610 SE	NB300-529MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-529-0.025ml

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NBP1-22444

Cav3.2 Antibody (S55/10) - BSA Free

(1 Review)

(10 Publications)

0.1 mg

NBP1-22444

Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot using rat brain membranes and NBP1-22444 to target Cav3.2 channels visible at about 260 kDa.

Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.1111/acel.13134) licensed under a CC-BY license.

Immunohistochemistry-Frozen: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.

$Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Age attenuates the role of CaV3.2 channels in Ca2+ spark generation & decreases CaV3.2 protein expression in VSMC. (a), Ca2+ fluorescence images of a Fluo‐4‐AM–loaded VSMC from a young mouse & time course of Ca2+ fluorescence changes in the cellular ROI (upper panel). Cell boundary is marked with dashed line. (b), same as (a) but in the presence of Ni2+ (50 µM). (c), same as (a) but in a VSMC from an old mouse. (d), same as (c) but in the presence of Ni2+ (50 µM). (e, f), summary of the results. Ca2+ spark frequency (e) & fraction of cells producing Ca2+ sparks (f) in VSMCs from young mice (n = 102), in VSMCs from young mice cells incubated with Ni2+ (n = 85), in VSMCs from aged mice (n = 129), & in VSMCs from aged mice cells incubated with Ni2+ (n = 127). Cells were isolated from 4 mice in each group; 25–40 cells were recorded & analyzed from each mouse. VSMC, vascular smooth muscle cell. (g), Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. n.s., not significant Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32187825), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-22444AF350
Alexa Fluor 405	NBP1-22444AF405
Alexa Fluor 488	NBP1-22444AF488
Alexa Fluor 532	NBP1-22444AF532
Alexa Fluor 594	NBP1-22444AF594
Alexa Fluor 647	NBP1-22444AF647
Alexa Fluor 700	NBP1-22444AF700
Alexa Fluor 750	NBP1-22444AF750
Allophycocyanin	NBP1-22444APC
Biotin	NBP1-22444B
CoraFluor 1	NBP1-22444CL1
DyLight 350	NBP1-22444UV
DyLight 405	NBP1-22444V
DyLight 488	NBP1-22444G
DyLight 550	NBP1-22444R
DyLight 594	NBP1-22444DL594
DyLight 650	NBP1-22444C
DyLight 680	NBP1-22444FR
DyLight 755	NBP1-22444IR
FITC	NBP1-22444F
HRP	NBP1-22444H
Janelia Fluor 525	NBP1-22444JF525
Janelia Fluor 549	NBP1-22444JF549
Janelia Fluor 646	NBP1-22444JF646
Janelia Fluor 669	NBP1-22444JF669
PE	NBP1-22444PE
PerCP	NBP1-22444PCP
mFluor Violet 450 SE	NBP1-22444MFV450
mFluor Violet 500 SE	NBP1-22444MFV500
mFluor Violet 610 SE	NBP1-22444MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-22444SS

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NBP1-85641

Chorein Antibody - BSA Free

(10 Publications)

0.1 ml

NBP1-85641

Immunohistochemistry-Paraffin: Chorein Antibody [NBP1-85641] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Chorein Antibody [NBP1-85641] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Western blotting analysis for epididymal sperm and testis lysates was performed to compare the protein levels of ChAcDel/Del mice (n = 5) with those of WT mice (n = 5). (A) Proteins were visualized using the MemCode Reversible Protein Stain Kit after separation by SDS-PAGE and transfer to PVDF membranes. The contrast of the sperm lysates image was increased by a factor of two to enhance visibility. Sperm lysates exhibited different patterns of protein expression between WT and ChAcDel/Del mice. Testis lysates exhibited similar patterns between both groups. (B) Representative results of Western blotting analysis are shown. (C) Relative quantification of Western blotting analysis is presented with white and black bars indicating WT and ChAc mice, respectively. The Mann–Whitney U test was performed for statistical analysis. WT sperm lysates exhibited significantly higher levels of IDH3A and PGK2 than ChAcDel/Del sperm lysates. Interestingly, WT mice exhibited significantly lower levels of TOM20 than ChAcDel/Del mice. No differences were observed between WT and ChAcDel/Del testis lysates. * p 2 repeats. Each point is the mean value from 3 replicates, and error bars represent +/-SD. Some error bars are contained within the point and therefore not visible. (C) Western blot analysis of PLA2G15 (∼43 kDa) and GAPDH (∼36 kDa) expression in whole-cell lysates of HeLa control cells and HeLa PLA2G15 monoclonal KO cells. The cell line showing complete abrogation of PLA2G15 expression (clone 36) was tested for resistance to beta hc. (D) An LDH cytotoxicity assay with HeLa PLA2G15 KO clone 36, demonstrating no effect of knocking out PLA2G15 on susceptibility to beta hc. The results shown are from one representative assay of >2 repeats. Each point is the mean value from 3 replicates, and error bars represent +/-SD. Some error bars are contained within the point and therefore not visible. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35196804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

VPS13A and XK are expressed in human striatum. (A) Domain organization of two splice variants of VPS13A. (B) COS7 cells coexpressing VPS13A(splice variant B)^Halo and GFP-XK, showing no colocalization as expected due to the lack of the PH domain. The Top and Bottom images show the same microscopy field. (C) qPCR quantitation of transcript levels of splice variants A and B of VPS13A, relative to all VPS13A variants, in human caudate samples. Splice variant A is expressed at higher levels than variant B, as opposed to what was reported in mice. VPS13A isoform levels in each case were normalized to housekeeping gene ACTB, with delta Ct for each isoform calculated from the qPCR cycle threshold (Ct) of the VPS13A isoform versus the ACTB Ct. Values were further normalized to the transcript levels of all VPS13A variants (2-delta delta Ct) obtained by PCR amplification of an exon present in all reported VPS13A variants. (D) Violin plots of single nucleus RNA-seq data of human caudate samples showing preferential expression of VPS13A and XK in neurons, including MSNs where VPS13A has the highest expression levels. (E) COS7 cells coexpressing XKKKR→AAA^Twin-Strep, VPS13A^Halo, and GFP-JPH3. The figure, which shows a view in a plane close to the substrate, reveals that both VPS13A^Halo and GFP-JPH3 localize to ER–PM contacts but do not overlap. E′ shows at high magnification the area enclosed by a rectangle in E, and E′′ shows an orthogonal view along the dashed line of E′. (Scale bar, 5 μm.) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35994651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85641-25ul

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NBP1-88219

Secretagogin Antibody - BSA Free

(8 Publications)

0.1 ml

NBP1-88219

Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.

Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human pancreas shows strong cytoplasmic and nuclear positivity in islets of Langerhans.

Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human rectum shows strong cytoplasmic positivity in peripheral nerve / ganglion.

Immunohistochemistry: Secretagogin Antibody [NBP1-88219] - Staining of mouse brain shows positivity in fasciola cinereum.

Immunohistochemistry: Secretagogin Antibody [NBP1-88219] - Staining of mouse hypothalamus shows positivity in the paraventricular hypothalamic nucleus.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-88219-25ul

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NB100-98886

Vanilloid R1/TRPV1 Antibody

(7 Publications)

0.1 ml

NB100-98886

Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um

<b>Biological Strategies Validation. </b>Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Transient receptor potential vanilloid type 1 (Vanilloid R1/TRPV1) protein expression pattern in cortical microglia after rFS. Western blot analysis of total protein (A) and membrane protein expression (B) of Vanilloid R1/TRPV1 in three samples from rFSs group's mice. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-02577

Profilin 1 Antibody (OTI1D5)

(3 Publications)

0.1 ml

NBP2-02577

Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.

Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then analyzed by flow cytometry.

Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.

Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.

Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.

Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73605AF350
Alexa Fluor 405	NBP2-73605AF405
Alexa Fluor 488	NBP2-73605AF488
Alexa Fluor 532	NBP2-73605AF532
Alexa Fluor 594	NBP2-73605AF594
Alexa Fluor 647	NBP2-73605AF647
Alexa Fluor 700	NBP2-73605AF700
Alexa Fluor 750	NBP2-73605AF750
Allophycocyanin	NBP2-73605APC
Biotin	NBP2-73605B
CoraFluor 1	NBP2-73605CL1
DyLight 350	NBP2-73605UV
DyLight 405	NBP2-73605V
DyLight 488	NBP2-73605G
DyLight 550	NBP2-73605R
DyLight 594	NBP2-73605DL594
DyLight 650	NBP2-73605C
DyLight 680	NBP2-73605FR
DyLight 755	NBP2-73605IR
FITC	NBP2-73605F
HRP	NBP2-73605H
Janelia Fluor 525	NBP2-73605JF525
Janelia Fluor 549	NBP2-73605JF549
Janelia Fluor 646	NBP2-73605JF646
Janelia Fluor 669	NBP2-73605JF669
PE	NBP2-73605PE
PerCP	NBP2-73605PCP
mFluor Violet 450 SE	NBP2-73605MFV450
mFluor Violet 500 SE	NBP2-73605MFV500
mFluor Violet 610 SE	NBP2-73605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73605

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NBP1-75522

Orai1 Antibody (3F6H5) - BSA Free

(6 Publications)

0.1 mg

NBP1-75522

Immunohistochemistry: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 5 u/mL.

Immunohistochemistry: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-75522AF350
Alexa Fluor 405	NBP1-75522AF405
Alexa Fluor 488	NBP1-75522AF488
Alexa Fluor 532	NBP1-75522AF532
Alexa Fluor 594	NBP1-75522AF594
Alexa Fluor 647	NBP1-75522AF647
Alexa Fluor 700	NBP1-75522AF700
Alexa Fluor 750	NBP1-75522AF750
Allophycocyanin	NBP1-75522APC
Biotin	NBP1-75522B
CoraFluor 1	NBP1-75522CL1
DyLight 350	NBP1-75522UV
DyLight 405	NBP1-75522V
DyLight 488	NBP1-75522G
DyLight 550	NBP1-75522R
DyLight 594	NBP1-75522DL594
DyLight 650	NBP1-75522C
DyLight 680	NBP1-75522FR
DyLight 755	NBP1-75522IR
FITC	NBP1-75522F
HRP	NBP1-75522H
Janelia Fluor 525	NBP1-75522JF525
Janelia Fluor 549	NBP1-75522JF549
Janelia Fluor 646	NBP1-75522JF646
Janelia Fluor 669	NBP1-75522JF669
PE	NBP1-75522PE
PerCP	NBP1-75522PCP
mFluor Violet 450 SE	NBP1-75522MFV450
mFluor Violet 500 SE	NBP1-75522MFV500
mFluor Violet 610 SE	NBP1-75522MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-75522-0.025mg

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NBP1-87493

Monoamine Oxidase B Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-87493

Western Blot: Monoamine Oxidase B Antibody [NBP1-87493] - Analysis in human liver tissue.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in Leydig cells.

Glutamate-driven astrocytic MAO-B leading to reactive astrogliosis and astrocytic scar formation. a Schematic representation of glutamate-driven astrocytic MAO-B inducing reactive astrogliosis and astrocytic scar formation. b-d Western blot analysis for EAAT1 and MAO-B expression in astrocyte monocultures upon glutamate treatment. Glutamate treatment increased MAO-B expression in astrocytes. Blockage of glutamate transport activity by using glutamate transporter inhibitor (TBOA) decreased MAO-B expression. e, f Reactivity of astrocyte monocultures upon glutamate treatment assessed by immunostaining with GFAP. Inhibiting glutamate transport (TBOA) or MAO-B (KDS2010) decreased GFAP expression. g ELISA for CSPGs deposited from reactive astrocytes. h, i Immunofluorescence images and quantification of GFAP intensity in the mouse tissues. j, k Immunofluorescence images and quantification of CSPGs intensity in the mouse tissues. Quantitative data were presented as means +/- SD (n = 3, unless otherwise noted). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. p values were calculated by two-tailed unpaired Student's t-test for comparisons between two groups, and by one-way ANOVA for multiple comparisons. Scale bars represent 100 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37468961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

MAO-B-positive mitovesicles are more numerous in the brain than MAO-A-positive mitovesicles. (A, B) Representative flow cytometry analyses of Fr8 brain EVs (mitovesicle-enriched, second columns) immunolabeled with antibodies against MAO-A (A) and MAO-B (B). The left columns show the same analyses performed on a 1:1:1 v/v mixture of Fr1, Fr2, and Fr3 EVs (microvesicle-enriched) isolated from the same brains (negative control). The right columns show mitovesicles incubated with the secondary antibody only. The gating strategy is shown for all conditions (first rows). Both the y- and the x-axis are in a log10 scale. 20,000 EVs were acquired for each experiment. FSC-A: forward scatter– area. SSC-A: side scatter– area. Ab: antibody. (C) Percentage of mitovesicles positive for either MAO-A or MAO-B. n = 3 mice per group. The percentage of positive EVs was determined on the basis of the secondary antibody control gate of each sample (set to 0.8% maximum). Bars: mean +/- SEM. Statistical test used: two-tailed, unpaired Student’s t-test. MAO-A vs. MAO-B, P = 0.0004. *** P < 0.001 Image collected and cropped by CiteAb from the following open publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00721-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-89370

S100A10 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-89370

Staining of human lung shows strong membranous positivity in macrophages.

<b>Genetic Strategies Validation. </b>Analysis in human lung and skeletal muscle tissues using NBP1-89370 antibody. Corresponding S100A10 RNA-seq data are presented for the same tissues.

Staining of human small intestine shows strong membranous positivity in glandular cells.

Staining of human skin shows strong membranous positivity in squamous epithelial cells.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines Caco-2 and HEK293 using Anti-S100A10 antibody. Corresponding S100A10 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

<b>Genetic Strategies Validation. </b>Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-S100A10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

The AT2-blocker PD123319 induces S100A10 expression in astrocytes with increased proliferation whereas Telmisartan does not affect S100A10 or Ki67 expression. *p < 0.05, **p < 0.01, and ***p < 0.001 compared different experimental groups as marked by horizontal bar; graphs depict mean values +/- standard error of the mean (SEM). A Experimental timeline. Three to five days after subculturing, astrocytes were left unstimulated or treated with 10 uM telmisartan or 10 uM PD123319 for 48 h. They were then exposed to 2 h of oxygen–glucose deprivation (OGD) before they were allowed to recover in regular culture medium for 24 h. Afterward, the cells were used for further experiments. Unstimulated astrocytes, which were exposed to 2 h of OGD, served as controls. B S100A10 marker expression in untreated (unstimulated) or OGD-treated (2 h of OGD) astrocytes with or without the additional preincubation with 10 uM telmisartan or 10 uM PD123319. C Proliferation rate of untreated (unstimulated) and OGD-treated (2 h OGD) astrocytes, with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h as assessed by mRNA-ki67 expression. D ATP concentration of untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h. E Expression of the connexin Cx43 in untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan and 10 uM PD123319 over 48 h. F Representative immunocytochemical stainings of astrocytes with Glia Fibrillary Acidic Protein (GFAP) + (green) and S100A10 + (red) in unstimulated (control), PD123319-preincubated (PD123319), or after 2 h of OGD (OGD + Control). Hoechst stained all cell nuclei blue; scale bars = 50 um Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38926677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89370-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Myosin 5a Antibody - BSA Free

Bassoon Antibody (SAP7F407) - BSA Free

Mitofusin 1 Antibody - BSA Free

SERCA2 ATPase Antibody (IID8) - BSA Free

Cav3.2 Antibody (S55/10) - BSA Free

Chorein Antibody - BSA Free

Secretagogin Antibody - BSA Free

Vanilloid R1/TRPV1 Antibody

Profilin 1 Antibody (OTI1D5)

Orai1 Antibody (3F6H5) - BSA Free

Monoamine Oxidase B Antibody - BSA Free

S100A10 Antibody - BSA Free