| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55376SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89113-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22164SS | |||
| Azide and BSA Free | NBP2-80954 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62566-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56351SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47254SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78162SS | |||
| Azide and BSA Free | NBP2-81010 | |||
![Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Total protein from HeLa, MCF7 and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TARDBP in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Western-Blot-NB110-55376-img0012.jpg "Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Total protein from HeLa, MCF7 and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TARDBP in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with TDP-43/TARDBP Antibody conjugated to Alexa Fluor 488 (NB110-55376AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-TARDBP at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0011.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody [NB110-55376] - Analysis of FFPE human placenta using TDP-43 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunohistochemistry-Paraffin-NB110-55376-img0010.jpg)
![Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in HeLa WCE.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Western-Blot-NB110-55376-img0004.jpg)
![Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Western-Blot-NB110-55376-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in MCF-7 cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Analysis using the DyLight 550 conjugate of NB110-55376. Staining of Human iPS derived neurons, fixed in 4% formaldehyde solution. NB110-55376R was diluted 1:200. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Blue dots in TDP staining is Hoechst.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Subcellular distribution of TDP-43 and CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55376-img0013.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues . Corresponding FUS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0027.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues . Corresponding FUS RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: FUS Antibody [NBP1-89113] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-FUS antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NBP1-89113-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody [NBP1-89113] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89113-img0028.jpg)
![Western Blot: FUS Antibody [NBP1-89113] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NBP1-89113-img0011.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human cerebellum shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0022.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0023.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0024.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human liver shows moderate nuclear positivity in bile duct cells while hepatocytes are negative.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0025.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0026.jpg)
![Western Blot: NMDAR2A Antibody [NB300-105] - GluN2B to GluN2A NMDAR switch and Ppt1-/--induced synaptic deficits are recapitulated in primary cortical neurons. (B) Representative immunoblot (top) and quantification of GluN2A levels (bottom) in WT and Ppt1-/- neurons at DIV7, 10, and 18. (D) Representative immunoblot (top) and quantification of the GluN2A/2B ratio (bottom) in WT and Ppt1-/- neurons at DIV7, 10, and 18. For all experiments in Figure 5, Ppt1-/- and WT were compared (n = 2 independent experiments with two repetitions/group) at each time point using t-test and the significance indicated as follows: *p<0.05 where indicated. Error bars represent s.e.m. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/40316) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-Western-Blot-NB300-105-img0006.jpg "Western Blot: NMDAR2A Antibody [NB300-105] - GluN2B to GluN2A NMDAR switch and Ppt1-/--induced synaptic deficits are recapitulated in primary cortical neurons. (B) Representative immunoblot (top) and quantification of GluN2A levels (bottom) in WT and Ppt1-/- neurons at DIV7, 10, and 18. (D) Representative immunoblot (top) and quantification of the GluN2A/2B ratio (bottom) in WT and Ppt1-/- neurons at DIV7, 10, and 18. For all experiments in Figure 5, Ppt1-/- and WT were compared (n = 2 independent experiments with two repetitions/group) at each time point using t-test and the significance indicated as follows: *p<0.05 where indicated. Error bars represent s.e.m. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/40316) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: NMDAR2A Antibody [NB300-105] - Hyperpalmitoylation of Fyn kinase and GluN2B is reversed in Ppt1-/- primary cortical neurons by palmitoylation inhibitor treatment. Representative post-APEGS immunoblot of GluN2A (NMDAR2A) with beta-actin loading control. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/40316), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-Western-Blot-NB300-105-img0007.jpg)
![Immunohistochemistry: NMDAR2A Antibody [NB300-105] - Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-Immunohistochemistry-NB300-105-img0004.jpg)
![Western Blot: NMDAR2A Antibody [NB300-105]](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-Western-Blot-NB300-105-img0005.jpg)
![Western Blot: NMDAR2A Antibody [NB300-105] - NMDAR subunit composition is immature in whole lysates from Ppt1-/- visual cortex.(A) Representative immunoblots of the GluN2A in whole lysates across age & genotype as indicated (top) & quantification of band density (bottom) normalized to beta -actin loading control within lane. (B) Representative immunoblots of the GluN2B in whole lysates across age & genotype as indicated (top) & quantification of band density (bottom) normalized to beta -actin loading control within lane. (C) Representative immunoblots of GluN2A & GluN2B (top) from whole lysates across age & genotype & quantification of the ratio of GluN2A/GluN2B band density within animal (bottom). (D) Representative immunoblots of GluN1 in whole lysates across age & genotype as indicated (top) & quantification of band density (bottom) normalized to beta -actin loading control within lane. (E) Representative immunoblot from whole lysates of PPT1 across age & genotype as indicated (top) & protein expression level (bottom) normalized to beta -actin. For experiments in Figure 2—figure supplement 1A–C, Ppt1-/- & WT were compared (n = 4 independent experiments/animals with two repetitions/group) at each age using t-test & the significance is indicated: *p<0.05. In Figure 2D, WT expression levels at each age were compared (n = 4 independent experiments/animals with two repetitions/group) by ANOVA followed by Tukey’s post-hoc test. Significance between ages is indicated: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Error bars represent s.e.m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30946007), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-105_rabbit-polyclonal-nmdar2a-antibody-310202416203711.jpg)
![Western Blot: NMDAR2A Antibody [NB300-105] - GluN2B to GluN2A NMDAR switch & Ppt1-/--induced synaptic deficits are recapitulated in primary cortical neurons.(A) Representative immunoblot (top) & quantification of GluN2B levels in WT & Ppt1-/- neurons at DIV7, 10, & 18. (B) Representative immunoblot (top) & quantification of GluN2A levels (bottom) in WT & Ppt1-/- neurons at DIV7, 10, & 18. (C) Representative immunoblot (top) & quantification of PSD-95 levels (bottom) in WT & Ppt1-/- neurons at DIV7, 10, & 18. (D) Representative immunoblot (top) & quantification of the GluN2A/2B ratio (bottom) in WT & Ppt1-/- neurons at DIV7, 10, & 18. For all experiments in Figure 5, Ppt1-/- & WT were compared (n = 2 independent experiments with two repetitions/group) at each time point using t-test & the significance indicated as follows: *p<0.05 where indicated. Error bars represent s.e.m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30946007), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-105_rabbit-polyclonal-nmdar2a-antibody-31020241620374.jpg)
![Western Blot: NMDAR2A Antibody [NB300-105] - NMDAR subunit composition is immature in whole lysates from Ppt1-/- visual cortex.(A) Representative immunoblots of the GluN2A in whole lysates across age & genotype as indicated (top) & quantification of band density (bottom) normalized to beta -actin loading control within lane. (B) Representative immunoblots of the GluN2B in whole lysates across age & genotype as indicated (top) & quantification of band density (bottom) normalized to beta -actin loading control within lane. (C) Representative immunoblots of GluN2A & GluN2B (top) from whole lysates across age & genotype & quantification of the ratio of GluN2A/GluN2B band density within animal (bottom). (D) Representative immunoblots of GluN1 in whole lysates across age & genotype as indicated (top) & quantification of band density (bottom) normalized to beta -actin loading control within lane. (E) Representative immunoblot from whole lysates of PPT1 across age & genotype as indicated (top) & protein expression level (bottom) normalized to beta -actin. For experiments in Figure 2—figure supplement 1A–C, Ppt1-/- & WT were compared (n = 4 independent experiments/animals with two repetitions/group) at each age using t-test & the significance is indicated: *p<0.05. In Figure 2D, WT expression levels at each age were compared (n = 4 independent experiments/animals with two repetitions/group) by ANOVA followed by Tukey’s post-hoc test. Significance between ages is indicated: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Error bars represent s.e.m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30946007), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-105_rabbit-polyclonal-nmdar2a-antibody-310202416205181.jpg)
![Western Blot: NMDAR2A Antibody [NB300-105] - GluN2B to GluN2A NMDAR switch & Ppt1-/--induced synaptic deficits are recapitulated in primary cortical neurons.(A) Representative immunoblot (top) & quantification of GluN2B levels in WT & Ppt1-/- neurons at DIV7, 10, & 18. (B) Representative immunoblot (top) & quantification of GluN2A levels (bottom) in WT & Ppt1-/- neurons at DIV7, 10, & 18. (C) Representative immunoblot (top) & quantification of PSD-95 levels (bottom) in WT & Ppt1-/- neurons at DIV7, 10, & 18. (D) Representative immunoblot (top) & quantification of the GluN2A/2B ratio (bottom) in WT & Ppt1-/- neurons at DIV7, 10, & 18. For all experiments in Figure 5, Ppt1-/- & WT were compared (n = 2 independent experiments with two repetitions/group) at each time point using t-test & the significance indicated as follows: *p<0.05 where indicated. Error bars represent s.e.m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30946007), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-105_rabbit-polyclonal-nmdar2a-antibody-310202416212371.jpg)
![Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.](http://images.novusbio.com/fullsize/SLC6A3-DAT1-Antibody-mAb16-Western-Blot-NBP2-22164-img0005.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.")
![Immunocytochemistry/Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/DAT1-Antibody-mAb16-Immunocytochemistry-Immunofluorescence-NBP2-22164-img0004.jpg)
![Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/DAT1-Antibody-mAb16-Immunohistochemistry-Paraffin-NBP2-22164-img0002.jpg)
![Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.](http://images.novusbio.com/fullsize/DAT1-Antibody-mAb16-Western-Blot-NBP2-22164-img0003.jpg)
![Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB300-106-img0006.jpg "Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.")
![Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Flow-Cytometry-NB300-106-img0004.jpg)
![Western Blot: NMDAR2B Antibody [NB300-106]](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB300-106-img0005.jpg)
![Western Blot: LRRK2 Antibody [NB110-58771] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human LRRK2 Polyclonal Antibody (Catalog # NB110-58771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Knockout-Validated-NB110-58771-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody [NB110-58771] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human LRRK2 Polyclonal Antibody (Catalog # NB110-58771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody [NB110-58771] - NB110-58771 in increasing amounts of human lymphoblast](http://images.novusbio.com/fullsize/LRRK2-Antibody-Western-Blot-NB110-58771-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB110-58771] - Immunofluorescence of Lrrk2 with NB110-58771 within (A) Substantia nigra neurons (B) Chains of migrating neuroblasts.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58771-img0008.jpg)
![Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771] - DAB Immunostaining in frozen sections of mouse brainstem.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-NB110-58771-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody [NB110-58771] - LRRK2 RNA levels 5 days after shRNA lentiviral transduction. The empty vector was used as the mock control. Lines represent the mean +/- SEM of three to four independent silencing experiments. A representative immunoblot analysis of LRRK2 protein levels 5 days after transduction is also shown. Beta-III-tubulin was the loading control. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Knockdown-Validated-NB110-58771-img0010.jpg)
![Western Blot: LRRK2 Antibody [NB110-58771] - Characterization of iPSC-derived DA neurons with LRRK2 mutations. a Diagram showing the DA differentiation protocol used for neural induction of human iPSC lines. b Temporal gene expression analyzed by qRT-PCR at three time points: induction (3 weeks), expansion (4–5 weeks), & maturation (>6 weeks). Each point represents the mean ± SEM of at least two independent differentiation experiments. c Representative images of mature neuronal cultures showing expression of neuronal ( beta III-tubulin, Tau, & alpha -synuclein) & dopaminergic (TH, NURR1) markers. Nuclei were counterstained with Hoechst. Scale bars: 50 μm. d Quantification of immunostainings. Data are represented as mean ± SEM of counts from at least two different lines for each genotype. e Representative western blot analyses of TH, Tau, & GFAP with beta III-tubulin as loading control in iPSC-derived mature neurons. f Representative immunoblots & quantification of LRRK2 expression in mature neuronal cultures. alpha -tubulin was the loading control & data were normalized to control WT neurons. Bars represent the mean ± SEM of at least two different lines per genotype. DIV days in vitro, GEL gelatin, POL poly-ornithine, FBN fibronectin, LMN laminin, N2 N2 supplement, bFGF basic fibroblast growth factor, SAG smoothened agonist, LDN LDN-193189, CHIR CHIR99021, SB SB431542, BDNF brain-derived neurotrophic factor, AA ascorbic acid, B27 B27 supplement, dbcAMP dibutyryl cyclic adenosine monophosphate, TGF beta III transforming growth factor beta III, GDNF glial derived neurotrophic factor. See Additional file 2 for uncropped blots Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58771_rabbit-polyclonal-lrrk2-antibody-31020241623559.jpg)
![Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Western-Blot-NBP2-62566-img0002.jpg "Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.")
![Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Immunohistochemistry-Paraffin-NBP2-62566-img0001.jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-284202314243..jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-672023103423..jpg)
![Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunofluorescence-NB300-139-img0006.jpg "Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.")
![Western Blot: alpha-Internexin Antibody [NB300-139] - Whole tissue lysates using rabbit pAb to alpha-internexin, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0007.jpg)
![Western Blot: alpha-Internexin Antibody [NB300-139] - The expression of a-internexin (INA) was analyzed by Western blotting. White bars are controls, grey bars are enucleated and black bars are anophthalmic mice. Results from V1 (left), V2M (middle) and V2L (right) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159320) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - IF Confocal analysis of SHSY5Y cells using alpha Internexin antibody (NB300-139, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0005.jpg)
![Western Blot: alpha Internexin Antibody [NB300-139] - Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kDa is also seen, apparently resulting from dimerization of alpha-internexin.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin CPCA-Peri (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Polyclonal alpha internexin, NB 300-139 (red) staining neuronal progenitor cells. The green stain shows the fibroblast marker, Plectin (not one of our antibodies).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0004.jpg)
![Western Blot: alpha-Internexin Antibody [NB300-139] - Proteins involved in morphogenesis.The expression of a-internexin (INA), collapsing response mediator protein 2 (CRMP2) & 4 (CRMP4), neurofilament-low (NFL) & tubuline-b2 (Tub beta 2) were analyzed by Western blotting. White bars are controls, grey bars are enucleated & black bars are anophthalmic mice. Results from V1 (left), V2M (middle) & V2L (right) are shown for each protein. * P < 0.05 & ** P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27410964), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-139_rabbit-polyclonal-alpha-internexin-antibody-310202415371950.jpg)
![Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.](http://images.novusbio.com/fullsize/GABA-A-R-delta-Antibody-Western-Blot-NB300-200-img0001.jpg "Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.")
![Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (//journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (//journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Immunohistochemistry-Paraffin-NB100-56351-img0010.jpg)
![Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0012.jpg)
![Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Simple-Western-NB100-56351-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0012.jpg "Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0009.jpg)
![Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/VLDLR-Antibody-6A6-Western-Blot-NBP1-78162-img0010.jpg "Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.")
![Immunocytochemistry/Immunofluorescence: VLDLR Antibody (6A6) [NBP1-78162] - VLDL R Antibody (6A6) [NBP1-78162] - VLDL Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Immunocytochemistry-Immunofluorescence-NBP1-78162-img0006.jpg)
![Immunohistochemistry: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor in mouse kidney.](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Immunohistochemistry-NBP1-78162-img0007.jpg)
![Western Blot: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor expression in mouse kidney.](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Western-Blot-NBP1-78162-img0008.jpg)