| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74569SS | |||
| Azide and BSA Free | NBP2-80758 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97417SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13249-0.2mg | |||
| BSA Free | NB120-13249-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56583SS | |||
| Azide and BSA Free | NBP2-80872 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2682SS | |||
![Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - HMGB1/HMG-1 expression became up-regulated in thyroid cancer and was associated with clinicopathologic features. Immunohistochemical staining of HMGB1/HMG-1 was performed for different tissues. TC, thyroid cancer; TA, thyroid adenoma; SG, simple goiter; N, normal thyroid; PTC, papillary thyroid carcinoma; FTC, follicular thyroid carcinoma. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-019-1328-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-Western-Blot-H00003146-M08-img0017.jpg "Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - HMGB1/HMG-1 expression became up-regulated in thyroid cancer and was associated with clinicopathologic features. Immunohistochemical staining of HMGB1/HMG-1 was performed for different tissues. TC, thyroid cancer; TA, thyroid adenoma; SG, simple goiter; N, normal thyroid; PTC, papillary thyroid carcinoma; FTC, follicular thyroid carcinoma. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-019-1328-3) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-Immunocytochemistry-Immunofluorescence-H00003146-M08-img0012.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-Immunohistochemistry-Paraffin-H00003146-M08-img0014.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of HMGB1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-Western-Blot-H00003146-M08-img0015.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M08), clone 2F6. Lane 1: HMGB1 transfected lysatE (25 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-Western-Blot-H00003146-M08-img0016.jpg)
![ELISA: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Detection limit for recombinant GST tagged HMGB1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-ELISA-H00003146-M08-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - HMGB1/HMG-1 regulated autophagy in thyroid cancer cells. FTC-133/TPC-1 cells were transfected with HMGB1/HMG-1 shRNA and control shRNA and then starved by HBSS for 2 h. And LC3-I/II (NB600-1384) level was assayed by Western blot. Image collected and cropped by CiteAb from the following publication (www.jeccr.biomedcentral.com/articles/10.1186/s13046-019-1328-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-2F6-Knockdown-Validated-H00003146-M08-img0018.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - HMGB1 regulated autophagy in thyroid cancer cells (a) FTC-133/TPC-1 cells were transfected with HMGB1 shRNA & control shRNA & then starved by HBSS for 2 h. & LC3-I/II level was assayed by Western blot; (b) FTC-133/TPC-1 cells were transfected with HMGB1 shRNA & control shRNA & then pre-treated for 1 h with pepstatin A (PA, 10 μM) & E64D (10 μM) as indicated. Cells were subsequently treated for 3 h with HBSS in continuous presence or absence PA/E64D inhibitors. LC3-I/II, Beclin1 & p62 levels were assayed by Western blot; (c) Ultrastructural features in FTC-133/TPC-1 cells transfected with HMGB1 shRNA & control shRNA after a 3-h treatment of HBSS. More autophagosomes were seen in control shRNA plus HBSS-treated cells than in cells treated with HMGB1 shRNA plus HBSS. Arrows indicated autophagosomes Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31331356), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00003146-m08_mouse-monoclonal-hmgb1-hmg-1-antibody-2f6-31020241548361.jpg)
![Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Western-Blot-NB110-74569-img0006.jpg "Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.")
![Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Immunohistochemistry-NB110-74569-img0004.jpg)
![Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Flow-Cytometry-NB110-74569-img0007.jpg)
![Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Western-Blot-NB110-74569-img0005.jpg)
![Western Blot: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - Analysis of TRPV1 in following cell lysates: (1) Ntera2, (2) HepG2, (3) MCF7, and (4) Cos7.](http://images.novusbio.com/fullsize/TRPV1-Antibody-Western-Blot-NBP1-97417-img0002.jpg "Western Blot: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - Analysis of TRPV1 in following cell lysates: (1) Ntera2, (2) HepG2, (3) MCF7, and (4) Cos7.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - TRPV1 Antibody [NBP1-97417] - TRPV1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/TRPV1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97417-img0003.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0004.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0003.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0011.jpg "Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.")
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0012.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified customer review.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0010.jpg)
![Western Blot: iNOS Antibody (K13-A) [NBP1-33780] - Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) & CD86 (B), & M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction in Saline/Sham, Saline/CPIP, Omega-3/CPIP, & Corn oil/CPIP groups. Data are expressed as mean ± SD of 6 animals per group, statistically assessed by the one-way ANOVA followed by Tukey’s test. ##p < 0.01 vs. the Saline/Sham group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35391753), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33780_rabbit-monoclonal-inos-antibody-k13-a-31020241535639.jpg)
![Simple Western: Ki67/MKI67 Antibody [NBP2-19012] - Detection of Ki67/MKI67 by Simple WesternTM. Simple Western lane view shows lysates of HeLa parental cell line and Ki67 knockout (KO) HeLa cell line. A specific band was detected for Ki67/MKI67 at approximately 312 kDa (as indicated) in the parental cell line, but is not detectable in the knockout HeLa cell line using 20 ug/mL of Rabbit Anti-Ki67/MKI67 Polyclonal Antibody (Catalog # NBP2-19012). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Knockout-Validated-NBP2-19012-img0009.jpg "<b>Genetic Strategies Validation. </b>Simple Western: Ki67/MKI67 Antibody [NBP2-19012] - Detection of Ki67/MKI67 by Simple WesternTM. Simple Western lane view shows lysates of HeLa parental cell line and Ki67 knockout (KO) HeLa cell line. A specific band was detected for Ki67/MKI67 at approximately 312 kDa (as indicated) in the parental cell line, but is not detectable in the knockout HeLa cell line using 20 ug/mL of Rabbit Anti-Ki67/MKI67 Polyclonal Antibody (Catalog # NBP2-19012). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP2-19012] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-19012 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19012-img0010.jpg)
![Western Blot: Ki-67/MKI67 Antibody [NBP2-19012] - Analysis of A431 (A), HeLa (B), Ntera2 (C), and HEK293 (D) cell lysate using Ki67 antibody (NBP2-19012) at 2 ug/ml.](http://images.novusbio.com/fullsize/Ki67-Antibody-Western-Blot-NBP2-19012-img0006.jpg)
![Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NBP2-19012] - Human breast tumor stained with Ki-67 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/Ki67-Antibody-Immunohistochemistry-Paraffin-NBP2-19012-img0003.jpg)
![Flow Cytometry: Ki-67/MKI67 Antibody [NBP2-19012] - Expression in actively growing Jurkat cells: Cells were stained with 0.2 ug of Ki-67 antibody (red) and isotype control (green) and positively stained population was identified using PE conjugated goat anti-rabbit IgG secondary antibody. Cells fixed and permeabilized using ice cold 70% ethanol were used in this intracellular staining protocol.](http://images.novusbio.com/fullsize/Ki67-Antibody-Flow-Cytometry-NBP2-19012-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ki-67/MKI67 Antibody [NBP2-19012] - Ki67 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.](http://images.novusbio.com/fullsize/Ki67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19012-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Ki67/MKI67 Antibody [NBP2-19012] - Cell proliferation of BCa cells treated by SIRT1-target-specific-siRNA (B) and negative-control-siRNA (A) was assayed by Ki-67 staining (green). Nuclei (blue) were stained by DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29147649/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19012-img0011.jpg)
![Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Western-Blot-NB120-13249-img0001.jpg "Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunocytochemistry-Immunofluorescence-NB120-13249-img0003.jpg)
![Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunohistochemistry-NB120-13249-img0002.jpg)
![Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0004.jpg "Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-Immunocytochemistry-Immunofluorescence-NB300-222-img0002.jpg)
![Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0003.jpg)
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Western-Blot-NB100-56583-img0004.jpg "Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0008.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0005.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0006.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of NFkB p105/p50 (2J10D7) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nb100-56583_mouse-monoclonal-nfkb-p105-p50-antibody-2j10d7-imgenex-img-5812a-2210202585646.jpg)
![Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Western-Blot-NB300-129-img0009.jpg "Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Analysis of HeLa cells stained with rabbit pAb to ubiquitin, NB300-129, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0008.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB300-129] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunohistochemistry-Paraffin-NB300-129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0001.jpg)
![Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Western-Blot-NB110-41404-img0003.jpg "Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41404-img0006.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-NB110-41404-img0002.jpg)
![Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - SUM159 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. L1CAM Antibody (NB100-2682) primary antibody: 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Western-Blot-NB100-2682-img0006.jpg "Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - SUM159 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. L1CAM Antibody (NB100-2682) primary antibody: 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with 1mM cAMP overnight to induce axon growth. Cells were fixed in 4% paraformaldehyde for 10 minutes and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti- NB100-2682 at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Immunocytochemistry-Immunofluorescence-NB100-2682-img0005.jpg)
![Immunohistochemistry-Frozen: L1CAM Antibody (UJ127.11) [NB100-2682] - Human pancreatic ductal adenocarcinoma (PDAC) tissue section stained with L1CAM antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Immunohistochemistry-Frozen-NB100-2682-img0007.jpg)
![Flow Cytometry: L1CAM Antibody (UJ127.11) [NB100-2682] - A surface stain was performed on HeLa cells with L1CAM Antibody (UJ127.11) NB100-2682 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Flow-Cytometry-NB100-2682-img0001.jpg)
![Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - Total protein from human HeLa, A431 and SHSY-5Y cell lines and human Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-L1CAM (NB100-2682) in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Western-Blot-NB100-2682-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with 1mM cAMP overnight to induce axon growth. Cells were fixed in 4% paraformaldehyde for 10 minutes and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti- NB100-2682 at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Immunocytochemistry-Immunofluorescence-NB100-2682-img0004.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127.11) [NB100-2682] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of L1CAM [UJ127.11] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining as observed in the cerebral cortex](http://images.novusbio.com/fullsize/L1CAM-Antibody-UJ127-11-Immunohistochemistry-Paraffin-NB100-2682-img0003.jpg)