| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76798SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88697-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-517SS | |||
![Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse brain. MEF2C stained by MEF2C antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunohistochemistry-Paraffin-NBP2-17260-img0018.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse brain. MEF2C stained by MEF2C antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse fore brain. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunohistochemistry-NBP2-17260-img0005.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Saos2 xenograft. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunohistochemistry-Paraffin-NBP2-17260-img0007.jpg)
![Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody (NBP2-17260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17260_rabbit-polyclonal-mef2c-antibody-910202420131220.jpg)
![MEF2C antibody immunoprecipitates MEF2C protein in IP experiments.IP samples: K562 whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of MEF2C protein by 4 ug MEF2C antibody (NBP2-17260)5 % SDS-PAGEThe immunoprecipitated MEF2C protein was detected by MEF2C antibody (NBP2-17260) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17260_rabbit-polyclonal-mef2c-antibody-immunoprecipitation-261120251821333.jpg)
![Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-89125-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Nrf1 Antibody [NBP1-89125] - The SIAH2-NRF1 axis facilitates hypoxia-induced metabolic reprogramming. Wild-type or K230R stably expressed MDA-MB-231 cells were cultured under normoxia or hypoxia for 36 h. Cells were harvested and analyzed by western blotting with indicated antibodies. Right: densitometric quantification of the indicated proteins. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-08618-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-89125-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP1-89125] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89125-img0005.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0011.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0009.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0010.jpg)
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19457-img0019.jpg "Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Frozen-NBP2-19457-img0007.jpg)
![N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody (NBP2-19457) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19457_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-2611202518224321.jpg)
![Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Western-Blot-NBP2-75510-img0005.jpg "Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0002.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0001.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0003.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0004.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0006.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Knockout-Validated-NBP1-76798-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76798-img0004.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-76798] - Human brain tissue with op18 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-76798-img0002.jpg)
![Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GNB1-Antibody-Western-Blot-NBP2-16699-img0002.jpg "Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Mouse testis. GNB1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GNB1-Antibody-Immunohistochemistry-Paraffin-NBP2-16699-img0008.jpg)
![Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Human lung papillory adenocarcinoma, using GNB1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/GNB1-Antibody-Immunohistochemistry-Paraffin-NBP2-16699-img0003.jpg)
![Immunoprecipitation: GNB1 Antibody [NBP2-16699] - Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 ug of GNB1 antibody. Western blot analysis was performed using GNB1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/GNB1-Antibody-Immunoprecipitation-NBP2-16699-img0005.jpg)
![Immunohistochemistry Whole-Mount:GNB1 Antibody [NBP2-16699] -Analysis (whole mount) of zebrafish embryo, using GNB1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-16699_rabbit-polyclonal-gnb1-antibody-immunohistochemistry-whole-mount-310202481127..jpg)
![GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNB1 protein stained by GNB1 antibody (NBP2-16699) diluted at 1:500.Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16699_rabbit-polyclonal-gnb1-antibody-immunocytochemistry-immunofluorescence-26112025194810.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy and affects multiple UPR-related TFs. In vitro day 3 B-cells were treated with 10 uM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors +/- standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 uM PF-429242 were evaluated for CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy and affects multiple UPR-related TFs. In vitro day 3 B-cells were treated with 10 uM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors +/- standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 uM PF-429242 were evaluated for CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0011.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Analysis in control (vector only transfected HEK293T lysate) and CREB3L2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0006.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Expression of CREB3L2 in differentiating primary human B-cells. Protein expression of CREB3L2 during in vitro differentiation of primary human B-cells. The day of culture is indicated at the top. Intact and dashed arrows show migration of full-length protein and cleaved C-terminus, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0007.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human kidney shows strong cytoplasmci positivity in cells in tubules.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0009.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0010.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Kinetic & dose analysis of S1P-specific inhibitor PF-429242 during the early phase of ASC generation. (A) In vitro activated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 3 & assessed for CREB3L2 processing at 12 h intervals. Representative results are shown from a total of 7 donors. (B) Samples from part (A) were assessed for cell number at the indicated time points. Displayed are the average cell number ± standard deviation derived from 7 donors. (C) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 3 & assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202415382439.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P-specific inhibitor PF-429242 blocks CREB3L2 processing. (A) Diagram of CREB3L2 structure with proteolytic cleavage sites. Act domain, Activation Domain. bZIP, Basic Leucine Zipper Domain. TM, Transmembrane Domain. (B) In vitro generated human ASCs were treated with 10 μM PF-429242 or vehicle control (dH2O) on the day that appears first (d6, d8, d10) & protein lysates generated on the day that appears subsequently (d8, d10, d13) were analyzed by Western blotting. Intact & dashed arrows show migration of CREB3L2 full-length protein & cleaved C-terminus, respectively. (C) In vitro differentiated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 6 & analyzed subsequently for phenotype on the indicated days. Live cells were evaluated for expression of B-cell markers CD19 & CD20 & ASC markers CD38 & CD138. Percentages of cells are shown within the quadrant gates. The experiment was performed with 4 donors, a representative donor is shown. (D) Samples treated as in part (B) were assessed for cell number using CountBright beads. (E) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 6 & assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control. Supernatants from samples in part (B) were analyzed by ELISA for production of (F) IgM & (G) IgG. Significance was determined by t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202416121935.jpg)
![Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-517-img0005.jpg "Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.")
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western Blot analysis of total lysates from asynchronous HN30 cells and MCF-12a 3 days post-transfection. NT: non-treated, OB: only buffer, EGFP: only pcDNA3.1/CT-GFP vector, NSP 5a3a/EGFP: pcDNA 3.1/CT-GFP and pcDNA 3.0 NSP 5a3a. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-517-img0007.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using NB100-517. 0.5ug/ml dilution, 15 second ECL detection.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-517-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-517-img0003.jpg)
![Immunoprecipitation: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated. NT: non-treated HN30 cells, a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunoprecipitation-NB100-517-img0006.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated & HN30 cells transfected with pcDNA 3.0 NSP 5a3a & pcDNA 3.1/CT-GFP for 3 daysNT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a, & f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-517_rabbit-polyclonal-nsp-5-alpha-3-alpha-antibody-310202415525234.jpg)
![Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NB110-75039-img0001.jpg "Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0002.jpg)