| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-318SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-317SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89762-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74215 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91920-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88948-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59311-25ug | |||
![Dual RNAscope ISH-IHC: xCT Antibody [NB300-318] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB300-318) at 0.25ug/mL with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.](http://images.novusbio.com/fullsize/xCT-Antibody-Dual-RNAscope-ISH-IHC-NB300-318-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: xCT Antibody [NB300-318] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB300-318) at 0.25ug/mL with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NB300-318-img0013.jpg)
![Western Blot: xCT Antibody [NB300-318] - Western blot analysis of protein isolated from subcutaneous tumors derived from in vivo growth of the clones relative to WT-derived tumors revealed that xCT levels remained low, phospho-STAT5 (p-STAT5) levels remained high, and phospho-STAT3 (p-STAT3) levels remained unchanged in the absence of SH-4-54. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0161202), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-318-img0015.jpg)
![Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.](http://images.novusbio.com/fullsize/xCT-Antibody-Immunohistochemistry-NB300-318-img0006.jpg)
![Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with NB300-318AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-318-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: xCT Antibody [NB300-318] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/xCT-Antibody-Immunocytochemistry-Immunofluorescence-NB300-318-img0005.jpg)
![Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with xCT Antibody NB300-318AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-318-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: xCT Antibody [NB300-318] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the increase in xCT expression with treatment.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-318-img0007.jpg)
![Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa with xCT Antibody NB300-318 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-318-img0008.jpg)
![Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/mL in HepG2 cell lysates and antibody at 25 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 63 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NB300-318-img0014.jpg)
![Western Blot: xCT Antibody - BSA Free [NB300-318] - Subcutaneous injection into nude mice revealed that (A) MDA-MB-231 SH-4-54-resistant clone #2 proliferated at a slower rate than its wild-type (WT) counterpart in vivo. (B) qPCR demonstrated that xCT mRNA levels were lower in tumours isolated from animals injected with clone #2 relative to WT cells (2 animals per treatment group). (C) Western blot analysis of protein isolated from subcutaneous tumours derived from in vivo growth of the clones relative to WT-derived tumours revealed that xCT levels remained low, phospho-STAT5 (p-STAT5) levels remained high, & phospho-STAT3 (p-STAT3) levels remained unchanged in the absence of SH-4-54. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0161202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-318_rabbit-polyclonal-xct-antibody-310202415392520.jpg)
![Western Blot: xCT Antibody - BSA Free [NB300-318] - A link between KSHV latency & the MC1-R signaling axis in skin-derived cell lines. (a) Western blot analysis of phosphorylated NF-kappa B p65, MC1-R, & TRP-1 in total cell lysates extracted from MeWo (a) or Mel1700 (b) cells either uninfected (control) or acutely infected with KSHV for 0.3 h, 1 h, 3 h, or 6 h. GAPDH was used as loading control. (c) ImageJ quantitation of the p65 band intensities in (a) & (b) relative to GAPDH controls. (d) Mel1700 cells were infected in 6-well plates with increasing volumes (mL/well) of concentrated supernatant containing infectious KSHV, & total RNA from infected cells was subjected to RT-PCR using primer sets for host anti-inflammatory MC1R, POMC, & SLC7A11. (e) Equal aliquots from the same RNA used in (d) were subjected to RT-PCR analysis for select viral latency & cell growth control genes (i.e., GPCR, LANA, & v-FLIP). (f) Western blot analysis of the melanoma cell marker, Melan A, & anti-inflammatory genes MC1-R, TRP1, & SLC7A11 in total cell lysates of uninfected (−) or chronically infected (+) long-term cultures of MeWo-KSHV & Mel1700-KSHV cells. GAPDH was used as an internal control for both the RT-PCR (e) & western blot assays. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24701351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-318_rabbit-polyclonal-xct-antibody-31020241621818.jpg)
![Western Blot: xCT Antibody - BSA Free [NB300-318] - A link between KSHV latency & the MC1-R signaling axis in skin-derived cell lines. (a) Western blot analysis of phosphorylated NF-kappa B p65, MC1-R, & TRP-1 in total cell lysates extracted from MeWo (a) or Mel1700 (b) cells either uninfected (control) or acutely infected with KSHV for 0.3 h, 1 h, 3 h, or 6 h. GAPDH was used as loading control. (c) ImageJ quantitation of the p65 band intensities in (a) & (b) relative to GAPDH controls. (d) Mel1700 cells were infected in 6-well plates with increasing volumes (mL/well) of concentrated supernatant containing infectious KSHV, & total RNA from infected cells was subjected to RT-PCR using primer sets for host anti-inflammatory MC1R, POMC, & SLC7A11. (e) Equal aliquots from the same RNA used in (d) were subjected to RT-PCR analysis for select viral latency & cell growth control genes (i.e., GPCR, LANA, & v-FLIP). (f) Western blot analysis of the melanoma cell marker, Melan A, & anti-inflammatory genes MC1-R, TRP1, & SLC7A11 in total cell lysates of uninfected (−) or chronically infected (+) long-term cultures of MeWo-KSHV & Mel1700-KSHV cells. GAPDH was used as an internal control for both the RT-PCR (e) & western blot assays. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24701351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-318_rabbit-polyclonal-xct-antibody-310202416212337.jpg)
![Western Blot: xCT Antibody [NB300-317] - Total protein from human HeLa, Huvec, HCT-116 and A549 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-317-img0011.jpg "Western Blot: xCT Antibody [NB300-317] - Total protein from human HeLa, Huvec, HCT-116 and A549 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: xCT Antibody [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ macrophages are round shaped and form clusters in MS patients, whereas in controls, CD68+ cells appear isolated and long shaped. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/xCT-Antibody-Immunohistochemistry-Paraffin-NB300-317-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: xCT Antibody [NB300-317] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the increase in xCT expression with treatment.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-317-img0012.jpg)
![Flow Cytometry: xCT Antibody [NB300-317] - An intracellular stain was performed on HeLa cells with NB300-317G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-317-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: xCT Antibody [NB300-317] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/xCT-Antibody-Immunocytochemistry-Immunofluorescence-NB300-317-img0005.jpg)
![Flow Cytometry: xCT Antibody [NB300-317] - An intracellular stain was performed on HeLa with NB300-317 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-317-img0013.jpg)
![Simple Western: xCT Antibody [NB300-317] - (1) ladder, (2) no lysate + xCT, 100ug/ml, (3) human brain frontal cortex membrane lysate,no primary antibody, (4-7) human brain lysates, 0.03mg/ml, xCT, 100ug/ml.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NB300-317-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. A. Triple immunofluorescence staining for xCT (green), CD68 (red) & Hoechst 33258 (blue) in spinal cord of control (left) & MS patients (right). A high expression of xCT was detected in CD68+ infiltrating macrophages (arrows) associated with blood vessels, which are virtually absent in controls. Note that overall xCT expression is enhanced in MS tissue. B. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ macrophages are round shaped & form clusters in MS patients, whereas in controls, CD68+ cells appear isolated & long shaped. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202415284530.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. A. Triple immunofluorescence staining for xCT (green), CD68 (red) & Hoechst 33258 (blue) in spinal cord of control (left) & MS patients (right). A high expression of xCT was detected in CD68+ infiltrating macrophages (arrows) associated with blood vessels, which are virtually absent in controls. Note that overall xCT expression is enhanced in MS tissue. B. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ macrophages are round shaped & form clusters in MS patients, whereas in controls, CD68+ cells appear isolated & long shaped. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202415392541.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is increased in the CNS of rats with EAE. A. Histogram showing the neurological score during the course of acute EAE induced in Lewis rats by immunization with myelin basic protein. The peak of neurological disability was at day 14 post-immunization, which was selected for obtaining tissue samples. B. xCT mRNA (left) & protein (right) expression in spinal cord from control & acute EAE rats, as assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5-6). C. Double immunofluorescence for xCT (green) & OX-42 (red), a marker of microglia & infiltrating macrophages. OX42+ cells express high xCT levels in acute EAE. Both meninges (asterisk in top) & infiltrating cells (bottom) in inflammatory foci show high levels of xCT in rat spinal cord with EAE as compared to controls. D. Microglial cells (OX42+ cells) of EAE rats have higher xCT levels in spinal cord than controls. Notice the difference between resting microglia in control rats, with ramified morphology (arrows in control) & microglia in EAE showing round shaped morphology, characteristic of its activated state (arrows in EAE). Scale bar = 20 μm.E. xCT mRNA (left) & protein (right) expression in spinal cord from control & chronic EAE mice, assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5). Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202416205190.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is increased in the CNS of rats with EAE. A. Histogram showing the neurological score during the course of acute EAE induced in Lewis rats by immunization with myelin basic protein. The peak of neurological disability was at day 14 post-immunization, which was selected for obtaining tissue samples. B. xCT mRNA (left) & protein (right) expression in spinal cord from control & acute EAE rats, as assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5-6). C. Double immunofluorescence for xCT (green) & OX-42 (red), a marker of microglia & infiltrating macrophages. OX42+ cells express high xCT levels in acute EAE. Both meninges (asterisk in top) & infiltrating cells (bottom) in inflammatory foci show high levels of xCT in rat spinal cord with EAE as compared to controls. D. Microglial cells (OX42+ cells) of EAE rats have higher xCT levels in spinal cord than controls. Notice the difference between resting microglia in control rats, with ramified morphology (arrows in control) & microglia in EAE showing round shaped morphology, characteristic of its activated state (arrows in EAE). Scale bar = 20 μm.E. xCT mRNA (left) & protein (right) expression in spinal cord from control & chronic EAE mice, assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5). Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202416205138.jpg)
![Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Western-Blot-NBP1-20136-img0002.jpg "Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.")
![Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP1-20136-img0015.jpg)
![Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Hippocampal GLT-1 and NR1 expression 3 days after SE with MyD88 inhibition. Sections from the hippocampi of mice in the CP group (A1-A3) and MIP group (B1-B3) 3 days after SE with GLT-1 immunoreactivity in astrocytes and neuronal processes. (A4, B4) Higher magnification of the boxes in (A3) and (B3). Scale bars: (A1-A3, B1-B3) 100 um; (A4, B4) 50 um. SE = status epilepticus, DG=dentate gyrus, hippocampal regions CA3 and CA1, CP = control peptide, MIP = MyD88 inhibitory peptide. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30112701/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT2-GLT1 Antibody - Azide Free-Immunohistochemistry-NBP1-20136-img0016.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0003.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0004.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0005.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0006.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0007.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0008.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0009.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0010.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0011.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0012.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0013.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0014.jpg)
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Frozen-NBP2-20857-img0009.jpg "Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - CL1-5 xenograft, using VGAT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-20857-img0003.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - VIAAT/SLC32A1/VGAT antibody [N1N2], N-term detects VIAAT/SLC32A1/VGAT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VIAAT/SLC32A1/VGAT stained by VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-910202420134040.jpg)
![Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-western-blot-261120251825265.jpg)
![Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-western-blot-261120251826364.jpg)
![VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: VGAT protein stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-immunohistochemistry-frozen-261120251825312.jpg)
![VGAT antibody [N1N2], N-term detects VGAT protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-immunocytochemistry-immunofluorescence-26112025196525.jpg)
![VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGAT protein stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-immunocytochemistry-immunofluorescence-2611202518491317.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Fetal Brain Lane A: Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/Lane Gel Concentration: 0.12](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0015.jpg "Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Fetal Brain Lane A: Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/Lane Gel Concentration: 0.12")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59632] - Human alveolar cell.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59632-img0011.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0012.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - WB analysis of GLT1 in Hep3B WCL. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0013.jpg)
![Western Blot: EAAT2/GLT1 Antibody [NBP1-59632] - Human Fetal Brain lysates.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Western-Blot-NBP1-59632-img0014.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-59632] - Human Brain, cortex tissue at an antibody concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody-Immunohistochemistry-Paraffin-NBP1-59632-img0010.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-212024938316.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249425448.jpg)
![Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249362775.jpg)
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249415958.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (NBP3-25743) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249415994.jpg)
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249422680.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1616] (NBP3-25743) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249362722.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1616) - Azide and BSA Free [NBP3-25743] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (NBP3-25743) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-2120249403895.jpg)
![Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [HL1615] (NBP3-25743) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25743_rabbit-viaat-slc32a1-vgat-mab-hl1616-azide-and-bsa-free-western-blot-141120251832021.jpg)
![Western Blot: SLC7A8 Antibody (7D6) [NBP1-47989] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Western-Blot-NBP1-47989-img0001.jpg "Western Blot: SLC7A8 Antibody (7D6) [NBP1-47989] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.")
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0012.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0003.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0004.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded colon tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded endometrium tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Ovary tissue using anti-SLC7A8mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0009.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded pancreas tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0010.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] Staining of paraffin-embedded prostate tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0011.jpg)
![Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GAT3-Antibody-Western-Blot-NBP1-91920-img0006.jpg "Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebellum shows strong positivity in processes in cells in molecular layer.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0007.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0008.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0010.jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. Beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut3-Antibody-JA50-31-Western-Blot-NBP2-66872-img0007.jpg "Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. Beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.")
![Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of SH-SY5Y cells with Glucose Transporter GLUT3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Glut3-Antibody-JA50-31-Flow-Cytometry-NBP2-66872-img0005.jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/5,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)Lane 2: RAW264.7 cell lysate (20 ug/Lane)Lane 3: C6 cell lysate (20 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66872_rabbit-monoclonal-glut3-antibody-ja50-31-western-blot-6620249415..jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/1,000 dilution.Lane 1: HepG2-si NT cell lysate (10 ug/Lane)Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute; ECL: merk4-20% SDS-PAGE gel.NBP2-66872 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66872_rabbit-monoclonal-glut3-antibody-ja50-31-western-blot-6620249631..jpg)
![Simple Western: xCT Antibody [NBP2-88948] - Simple Western lane view shows a specific band for xCT using NBP2-88948 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NBP2-88948-img0002.jpg "<b>Biological Strategies Validation. </b>Simple Western: xCT Antibody [NBP2-88948] - Simple Western lane view shows a specific band for xCT using NBP2-88948 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: xCT Antibody [NBP2-88948] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of xCT antibody (NBP2-88948). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly cytoplasmic staining.](http://images.novusbio.com/fullsize/xCT-Antibody-Immunohistochemistry-Paraffin-NBP2-88948-img0001.jpg)
![Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (NBP2-59311) at 1:1000.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Western-Blot-NBP2-59311-img0004.jpg "Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (NBP2-59311) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (NBP2-59311) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Immunocytochemistry-Immunofluorescence-NBP2-59311-img0005.jpg)
![Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Western-Blot-NBP2-59311-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SNAT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Immunocytochemistry-Immunofluorescence-NBP2-59311-img0003.jpg)