| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40877SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87243-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87244-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-99585SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89271-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89270-25ul | |||
![Western Blot: Perilipin-2/ADFP Antibody - BSA Free [NB110-40877] - Western blot images (A) & quantifications (B–I) of muscle samples from WT & UCHL1 smKO mice for lipase ATGL (B), HSL (C), & MAGL (D), the fatty acid transport protein CD36 (E), the key lipid synthesis enzyme DGAT2 (F), perilipin 3 (G), perilipin 2 (H), & UCHL1 (I). n = 4-5 per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35464088), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40877_rabbit-polyclonal-perilipin-2-adfp-antibody-310202415171328.jpg)
![Western Blot: Perilipin-2/ADFP Antibody - BSA Free [NB110-40877] - Western blot images (A) & quantifications (B–G) of cell lysates from C2C12 myotubes with control or UCHL1 siRNA knockdown for perilipin 2 (B), perilipin 5 (C), CD36 (D), HSL (E), MAGL (F), & SDHB (G). n = 4 per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35464088), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40877_rabbit-polyclonal-perilipin-2-adfp-antibody-310202415384159.jpg)
![Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody - BSA Free [NB110-40877] - Lack of MT4-MMP in patrolling monocytes leads to the accumulation of Mafb+AIM+ macrophages in incipient atherosclerotic plaques. a Representative images of transverse sections of aortic sinus from Ldlr–/– mice transplanted with MT4-MMP+/+ (MT4+/+) or MT4-MMP–/– (MT4–/–) BM cells & fed a HFD for 1 week; sections were labeled for Mac3 (green), Mafb (red), & AIM (white), & with Hoechst (blue; nuclei); scale bar, 10 µm. b Number of Mac3+ cells (left), Mac3+Mafb+ cells (middle), & Mac3+Mafb+AIM+ cells (right) in the plaques of BM-transplanted Ldlr–/– mice fed a HFD for 1 week. c Representative images of transverse sections of aortic sinus from BM-transplanted Ldlr–/– mice fed a HFD for 1 week; sections were labeled for Mac3 (green), Mafb (red), & adipophilin (white), & with Hoechst (blue; nuclei); scale bar, 10 µm. d Relative % of adipophilin-positive & adipophilin-negative cells within the Mac3+Mafb+ population of BM-transplanted Ldlr–/– mice (1 week on HFD); n = 7 mice per genotype in two independent experiments. Data were tested by two-tailed Student’s t-test in b & by Fisher’s exact test in d. Results are expressed as mean ± SEM. *p < 0.05 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03351-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40877_rabbit-polyclonal-perilipin-2-adfp-antibody-310202416235522.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0011.jpg "Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.")
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0008.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0002.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0009.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0003.jpg)
![Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Sandwich-ELISA-H00055743-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87243 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87243 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human colon, kidney, lymph node and urinary bladder using Anti-NAPRT antibody NBP1-87243 (A) shows similar protein distribution across tissues to independent antibody NBP1-87244 (B).](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NAPRT1 Antibody [NBP1-87243] - Analysis using Anti-NAPRT antibody NBP1-87243 (A) shows similar pattern to independent antibody NBP1-87244 (B).](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Western-Blot-NBP1-87243-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NAPRT1 Antibody [NBP1-87243] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87243-img0002.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0015.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0012.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0013.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0014.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![Western Blot: ESCO2 Antibody [NB100-87021] - Detection of Human ESCO2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ESCO2-Antibody-Western-Blot-NB100-87021-img0007.jpg "Western Blot: ESCO2 Antibody [NB100-87021] - Detection of Human ESCO2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ESCO2 Antibody [NB100-87021] - Detection of human ESCO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 (lot 2) used for IP at 6 ug per reaction. ESCO2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-ESCO2 antibody from Company B. For blotting immunoprecipitated ESCO2, NB100-87021 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ESCO2-Antibody-Immunoprecipitation-NB100-87021-img0008.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87244 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87244-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87244 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human colon, kidney, lymph node and urinary bladder using Anti-NAPRT antibody NBP1-87244 (A) shows similar protein distribution across tissues to independent antibody NBP1-87243 (B).](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87244-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NAPRT1 Antibody [NBP1-87244] - Analysis using Anti-NAPRT antibody NBP1-87244 (A) shows similar pattern to independent antibody NBP1-87243 (B).](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Western-Blot-NBP1-87244-img0011.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87244-img0021.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87244-img0018.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87244-img0019.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87244] - Staining of human placenta shows moderate cytoplasmic positivity in tropoblastic cells.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87244-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Polyclonal NAPRT1 Antibody [NBP1-87244] - Immunoblot using primary cultured rat cardiomyocyte with NAPRT1 knockdown using the siRNA. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-87244_rabbit-polyclonal-naprt1-antibody-knockdown-validated-20122023112954.png)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")
![Western Blot: Importin-13 Antibody [NBP1-31508] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE Importin 13 antibody [C3], C-term dilution: 1:1000](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP1-31508-img0012.jpg "Western Blot: Importin-13 Antibody [NBP1-31508] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE Importin 13 antibody [C3], C-term dilution: 1:1000")
![Immunohistochemistry-Paraffin: Importin-13 Antibody [NBP1-31508] - CL1-5 xenograft, using Importin 13 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Immunohistochemistry-Paraffin-NBP1-31508-img0010.jpg)
![Western Blot: Importin-13 Antibody [NBP1-31508] - IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody. The precipitated IPO13 was detected by antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP1-31508-img0007.jpg)
![Western Blot: Importin-13 Antibody [NBP1-31508] - Analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate. B: IPO13 transfected lysate. 7.5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP1-31508-img0008.jpg)
![Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Western-Blot-NB110-99585-img0002.jpg "Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NB110-99585] - ACSL1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-99585-img0004.jpg)
![Simple Western: ACSL1 Antibody [NB110-99585] - Simple Western lane view shows a specific band for ACSL1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Simple-Western-NB110-99585-img0005.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 immunoreactivity and CDS activity localises to mitochondria in rat brain and liver. [A] Rat brain and [B] liver fractionated and analysed by CDS1 immunoreactivity by Western blot and for CDS activity. CDS activity was monitored in triplicate and error bars denote S.E.M. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; CDS, CDP-diacylglycerol synthase. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1388198117302421) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Western-Blot-H00001040-M01-img0009.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 immunoreactivity and CDS activity localises to mitochondria in rat brain and liver. [A] Rat brain and [B] liver fractionated and analysed by CDS1 immunoreactivity by Western blot and for CDS activity. CDS activity was monitored in triplicate and error bars denote S.E.M. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; CDS, CDP-diacylglycerol synthase. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1388198117302421) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01] - Analysis of monoclonal antibody to CDS1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Immunocytochemistry-Immunofluorescence-H00001040-M01-img0005.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 monoclonal antibody (M01), clone 2D10. Analysis of CDS1 expression in human liver.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Western-Blot-H00001040-M01-img0007.jpg)
![ELISA: CDS1 Antibody (2D10) [H00001040-M01] - Detection limit for recombinant GST tagged CDS1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-ELISA-H00001040-M01-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Knockdown of CDS1 with siRNA does not lead to a reduction in the p55 band. Mitochondrial fractions prepared from control, negative siRNA-treated and CDS1 siRNA-treated H9c2 cells were analysed for CDS1 immunoreactivity by Western blot. Image collected and cropped by CiteAb from the following publication (www.sciencedirect.com/science/article/pii/S1388198117302421?via%3Dihub) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Knockdown-Validated-H00001040-M01-img0010.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-31020241548365.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-310202415483622.jpg)
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-310202415482211.jpg)
![Western Blot: ACSL1 Antibody [NBP1-89271] - Analysis using Anti-ACSL1 antibody NBP1-89271 (A) shows similar pattern to independent antibody NBP1-89270 (B).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Western-Blot-NBP1-89271-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ACSL1 Antibody [NBP1-89271] - Analysis using Anti-ACSL1 antibody NBP1-89271 (A) shows similar pattern to independent antibody NBP1-89270 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NBP1-89271] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89271-img0010.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0017.jpg)
![Western Blot: ACSL1 Antibody [NBP1-89271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/ACSL1-Antibody-Western-Blot-NBP1-89271-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human kidney, liver, soft tissues and tonsil using Anti-ACSL1 antibody NBP1-89271 (A) shows similar protein distribution across tissues to independent antibody NBP1-89270 (B).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Analysis in human liver and tonsil tissues. Corresponding ACSL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0013.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human tonsil shows no cytoplasmic positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0014.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human soft tissues shows strong cytoplasmic positivity in adipocytes.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0015.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0016.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89271-img0018.jpg)
![Western Blot: ACSL1 Antibody [NBP1-89270] - Analysis using Anti-ACSL1 antibody NBP1-89270 (A) shows similar pattern to independent antibody NBP1-89271 (B).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Western-Blot-NBP1-89270-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ACSL1 Antibody [NBP1-89270] - Analysis using Anti-ACSL1 antibody NBP1-89270 (A) shows similar pattern to independent antibody NBP1-89271 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NBP1-89270] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89270-img0006.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Staining of human kidney, liver, soft tissues and tonsil using Anti-ACSL1 antibody NBP1-89270 (A) shows similar protein distribution across tissues to independent antibody NBP1-89271 (B).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Analysis in human liver and tonsil tissues. Corresponding ACSL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0012.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Staining of human soft tissues shows moderate to strong cytoplasmic positivity in adipocytes.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0013.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Staining of human tonsil shows no cytoplasmic positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0014.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0015.jpg)
![Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89270] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89270-img0017.jpg)