| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73226 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38499-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73228 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84556-25ul | |||
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Western-Blot-NBP2-02396-img0001.jpg "Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunocytochemistry-Immunofluorescence-NBP2-02396-img0008.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0007.jpg)
![Flow Cytometry: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells transfected with either Endothelial Lipase Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Flow-Cytometry-NBP2-02396-img0010.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0002.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0003.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0004.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human breast tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0005.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human Kidney tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0006.jpg)
![Immunoprecipitation: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Lane 1: Negative Control Lane 2: NBP2-02378 Lane 3: NBP2-02380 Lane 4:NBP2-02381 Lane 5: NBP2-02382 Lane 6: NBP2-02394 Lane 7: NBP2-02395 Lane 8: NBP2-02396 Lane 9: NBP2-02397](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunoprecipitation-NBP2-02396-img0009.jpg)
![Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Western-Blot-NBP2-02082-img0025.jpg "Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-02082-img0016.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0027.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0014.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0017.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0019.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0022.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0024.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0015.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-118-img0003.jpg "Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-118] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-118-img0002.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-118] - Analysis of Endothelial Lipase in ECV304 cells.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-118-img0004.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Western-Blot-NBP2-75653-img0006.jpg "Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate")
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunocytochemistry-Immunofluorescence-NBP2-75653-img0002.jpg)
![Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunohistochemistry-Paraffin-NBP2-75653-img0004.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Western-Blot-NBP2-75653-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunocytochemistry-Immunofluorescence-NBP2-75653-img0001.jpg)
![Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunohistochemistry-Paraffin-NBP2-75653-img0003.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP1-87353 (A) shows similar protein distribution across tissues to independent antibody NBP2-38499 (B).](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0019.jpg)
![Western Blot: PADI4 Antibody [NBP1-87353] - Analysis in control (vector only transfected HEK293T lysate) and pADI4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP1-87353-img0010.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human testis shows no nuclear positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human kidney shows no nuclear positivity as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human spleen shows strong nuclear positivity in cells in red pulp.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0022.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining in human bone marrow and kidney tissues using anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining in human bone marrow and kidney tissues using anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP2-38499 (A) shows similar protein distribution across tissues to independent antibody NBP1-87353 (B).](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0012.jpg)
![Western Blot: PADI4 Antibody [NBP2-38499] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP2-38499-img0006.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0003.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human liver.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0008.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human testis.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0009.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human spleen.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0010.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow using Anti-PADI4 antibody NBP2-38499.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0011.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Western-Blot-NBP2-46310-img0008.jpg "Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0007.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0001.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0002.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Human Ovary tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0005.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0006.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0003.jpg "Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Confocal imaging of HeLa cells using RXR alpha/NR2B1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-5220251731103.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR alpha/NR2B1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517344269.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 in the representative genomic region surrounding ECH1 gene.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517315534.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR alpha/NR2B1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517352787.jpg)
![Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP1-84556] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84556-img0008.jpg "Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP1-84556] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 2/CES2 Antibody [NBP1-84556] - CES2 Antibody [NBP1-84556] - Staining in human small intestine and pancreas tissues using anti-CES2 antibody. Corresponding CES2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP1-84556-img0011.jpg)
![Immunohistochemistry-Paraffin: CES2 Antibody [NBP1-84556] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP1-84556-img0009.jpg)
![Immunohistochemistry-Paraffin: CES2 Antibody [NBP1-84556] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP1-84556-img0010.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Western-Blot-H00023569-M01-img0001.jpg "Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (4D8) [H00023569-M01] - Analysis of monoclonal antibody to PADI4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Immunocytochemistry-Immunofluorescence-H00023569-M01-img0004.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Detection of enzymatically active peptidyl arginine deiminase (PAD) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA). A, Immunoblotting of albumin‐depleted SF samples from 8 RA patients shows presence of neutrophil elastase (NE), PAD4, & PAD2. B, PAD activity in the SF of patients with RA (n = 12) was significantly higher than that in the SF of patients with osteoarthritis (OA) (n = 9). C, PAD activity was observed in the OA SF & RA SF both at physiologic calcium concentrations & at higher calcium concentrations (5 mM). In B & C, symbols represent individual patients; horizontal bars show the median. D, PAD activity is significantly correlated with DNA levels in the SF of RA patients (n = 22). Data were analyzed using Spearman's rho test for correlation. ∗ = P < 0.05; ∗∗ = P < 0.01, by Mann‐Whitney U test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415482220.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Release of peptidyl arginine deiminases (PADs) into the supernatant during NETosis, & detection of enzymatically active PADs. A, DNA release in peripheral blood neutrophils from healthy donors that were left unstimulated or stimulated with 25 mM phorbol myristate acetate (PMA) was assessed for up to 240 minutes after stimulation. Bars show the mean ± SD of 8 samples per group. B, For isolation of neutrophil extracellular traps, supernatants (SN) of unstimulated (unst.) or stimulated (stim.) neutrophils were collected. Subsequently, the cells were washed 3 times with RPMI medium (W1–W3), & stimulated cells were incubated in the absence or presence of DNase I; unstimulated cells treated with DNase I served as a control. Bars show the mean ± SD DNA concentration (conc) in 7 samples per group. C, Proteins (PAD2, PAD4, & neutrophil elastase [NE]) were precipitated from the same supernatants of unstimulated or stimulated cells as described in B & analyzed by Western blotting. Citrullinated proteins (citr.prot.) were detected using chemical modification & anti–modified citrulline antibody. PAD2 & PAD4 antibodies were tested for cross‐reactivity using human (hu) recombinant PAD4 (rPAD4) (250 ng) & human skeletal muscle tissue lysate (15 μg). One representative blot of 4 independent experiments is shown for each group. D, PAD activity in supernatants of unstimulated & stimulated cells was compared. Symbols represent individual donors (n = 10); bars show the median. ∗ = P < 0.05; ∗∗ = P < 0.01, by Wilcoxon's matched pairs signed rank test. AFU = arbitrary fluorescence units; NS = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415485135.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-Western-Blot-NB100-1466-img0001.jpg "Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody [NB100-1466] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1466-img0002.jpg)
![Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-Flow-Cytometry-NB100-1466-img0003.jpg)
