DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." alt="CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25243 | |||
| BSA Free | NBP2-25237SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-146SS | |||
| Azide and BSA Free | NBP2-80977 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48532-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86177-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89557-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92286-25ul | |||
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # ![Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-45M4F9-Western-Blot-NBP2-25237-img0004.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-45M4F9-Immunohistochemistry-Paraffin-NBP2-25237-img0003.jpg)
![Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.](http://images.novusbio.com/fullsize/Thimet-Oligopeptidase-Antibody-4D6-Western-Blot-NB400-146-img0003.jpg "Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.")
![Immunohistochemistry: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Analysis of Thimet Oligopeptidase in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Thimet-Oligopeptidase-Antibody-4D6-Immunohistochemistry-NB400-146-img0004.jpg)
![Western Blot: Islet-1 Antibody [NBP2-14999] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0002.jpg "Western Blot: Islet-1 Antibody [NBP2-14999] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Islet-1 Antibody [NBP2-14999] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0006.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).](http://images.novusbio.com/fullsize/CD73-Antibody-4G4-Flow-Cytometry-NBP1-60135-img0001.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).")
![Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0012.jpg "Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82487-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0011.jpg)
![ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Samples were spiked with high concentrations of Mouse Caspase-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75014_mouse-caspase-1-elisa-kit-colorimetric-13220241692273.jpg "ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Samples were spiked with high concentrations of Mouse Caspase-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75014_mouse-caspase-1-elisa-kit-colorimetric-4320248582540.jpg)
![ELISA: Human Adropin ELISA Kit (Colorimetric) [NBP2-66433] - Samples were spiked with high concentrations of Human Adropin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66433_human-adropin-elisa-kit-colorimetric-13220241613034.jpg "ELISA: Human Adropin ELISA Kit (Colorimetric) [NBP2-66433] - Samples were spiked with high concentrations of Human Adropin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Adropin ELISA Kit (Colorimetric) [NBP2-66433] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66433_human-adropin-elisa-kit-colorimetric-4320249192872.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48532-img0006.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NBP2-48532] - Staining of human cell line Hep G2 shows localization to lipid droplets. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Western Blot-Perilipin-2/ADFP Antibody [NBP2-48532] -Analysis in human cell lines RT-4 and U-251MG using Anti-PLIN2 antibody. Corresponding PLIN2 RNA-seq data are presented for the same cell lines.](http://images.novusbio.com/fullsize/antibody/nbp2-48532_rabbit-polyclonal-perilipin-2-adfp-antibody-western-blot-285202412135..jpg)
![Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody [NBP1-86177] - Staining of human cell line A-431 shows localization to cytosol & rods & rings.](http://images.novusbio.com/fullsize/IMP-Dehydrogenase-2-IMPDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86177-img0008.jpg "Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody [NBP1-86177] - Staining of human cell line A-431 shows localization to cytosol & rods & rings.")
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Analysis in human liver and pancreas tissues. Corresponding APOE RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Analysis in human liver and pancreas tissues. Corresponding APOE RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human cerebral cortex, liver, pancreas and testis using Anti-APOE antibody NBP2-49450 (A) shows similar protein distribution across tissues to independent antibody NBP2-49565 (B).](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0009.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Analysis in human plasma.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP2-49450-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49450-img0008.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0015.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0012.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0013.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0014.jpg)
![Immunohistochemistry-Paraffin: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Analysis in human duodenum and tonsil tissues using NBP1-92286 antibody. Corresponding PRKAB2 RNA-seq data are presented for the same tissues](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Immunohistochemistry-Paraffin-NBP1-92286-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Analysis in human duodenum and tonsil tissues using NBP1-92286 antibody. Corresponding PRKAB2 RNA-seq data are presented for the same tissues")
![<b>Orthogonal Strategies Validation. </b>Western Blot: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Western-Blot-NBP1-92286-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92286-img0006.jpg)
![Immunohistochemistry-Paraffin: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Immunohistochemistry-Paraffin-NBP1-92286-img0012.jpg)
![Immunohistochemistry-Paraffin: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Immunohistochemistry-Paraffin-NBP1-92286-img0009.jpg)
![Immunohistochemistry-Paraffin: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Immunohistochemistry-Paraffin-NBP1-92286-img0010.jpg)
![Immunohistochemistry-Paraffin: AMPK beta 2/PRKAB2 Antibody [NBP1-92286] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/AMPK-beta-2-PRKAB2-Antibody-Immunohistochemistry-Paraffin-NBP1-92286-img0011.jpg)
