| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72759 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52533-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2911SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73226 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52532-0.025mg | |||
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16550_rabbit-polyclonal-fxr-nr1h4-antibody-western-blot-1220249182..jpg)
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Biochemical measurements & the mRNA & protein expression of top 5 NRs. A, Results of biochemical parameter measurements. Among the serum indicators, ALT in HFD group higher than it in NC group (P = .0004) & CSP group (P = .0222); the AST in HFD group higher than it in NC group (P = .0006) & CSP group (P = .0149); ALP in HFD group higher than it in NC group (P < .0001) & CSP group (P = .0013), & it lower in NC group than in CSP group (P = .0017); in HFD group, the TC level higher than it in NC group (P < .0001) & CSP group (P = .0003), & in NC group, it lower than it in CSP group (P < .0001); in HFD group, the TG level higher than it in NC group (P = .0011) & CSP group (P = .0476); in HFD group, the GLU level higher than it in NC group (P < .0001) & CSP group (P = .0002); & in HFD group, the HDL‐c level higher than it in NC group (P < .0001) & CSP group (P = .0022), & in NC group, it lower than it in CSP group (P < .0001) (n = 10). Among the liver indicators, in NC group, the TC level lower than it in HFD group (P = .0276) & CSP group (P = .0427); in NC group, the TG level lower than it in HFD group (P = .0427) & CSP group (P = .0448) (n = 6). B, The mRNA expression of top 5 NRs in liver. PPAR gamma in HFD group expressed at a higher level than it in NC group (P = .0072) & CSP group (P = .0489); FXR in HFD group expressed at a lower level than it in NC group (P = .0222) & CSP group (P = .0331); PPAR alpha in HFD group expressed lower than NC group (P = .0143) & CSP group (P = .0082); & RAR alpha in HFD group expressed at a higher level than it in NC group (P = .0001) & CSP group (P = .0012) (n = 10). C, Expression of top 5 NRs in liver measured by automatic WB analysis Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32189432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16550_rabbit-polyclonal-fxr-nr1h4-antibody-310202416113545.jpg)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Western Blot: Monoglyceride Lipase Antibody (OTI2B11) [NBP2-00735] - Analysis of extracts (10ug) from HeLa cell line, dilution(1:200).](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-OTI2B11-Western-Blot-NBP2-00735-img0024.jpg "Western Blot: Monoglyceride Lipase Antibody (OTI2B11) [NBP2-00735] - Analysis of extracts (10ug) from HeLa cell line, dilution(1:200).")
![Immunocytochemistry/Immunofluorescence: Monoglyceride Lipase Antibody (OTI2B11) [NBP2-00735] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunocytochemistry-Immunofluorescence-NBP2-00735-img0011.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Human prostate tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0021.jpg)
![Flow Cytometry: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Monoglyceride Lipase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Flow-Cytometry-NBP2-00735-img0012.jpg)
![Western Blot: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Monoglyceride Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Monoglyceride Lipase.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Western-Blot-NBP2-00735-img0022.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0013.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0014.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0015.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0016.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0017.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Human Kidney tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0018.jpg)
![Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody (2B11) [NBP2-00735] - Staining of paraffin-embedded Human pancreas tissue using anti-Monoglyceride Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Monoglyceride-Lipase-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-00735-img0019.jpg)
![Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mouse mAb against NIH/3T3 (1), Jurkat (2), A431 (3), and Hela (4) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Western-Blot-NBP2-52533-img0009.jpg "Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mouse mAb against NIH/3T3 (1), Jurkat (2), A431 (3), and Hela (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of Hela cells using PLCG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Immunofluorescence-NBP2-52533-img0002.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of paraffin-embedded esophagus tissues using PLCG1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Immunohistochemistry-Paraffin-NBP2-52533-img0004.jpg)
![Flow Cytometry: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of Raji cells using PLCG1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Flow-Cytometry-NBP2-52533-img0001.jpg)
![Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Western-Blot-NBP2-52533-img0006.jpg)
![Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Western-Blot-NBP2-52533-img0005.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of paraffin-embedded breast cancer tissues using PLCG1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-Immunohistochemistry-Paraffin-NBP2-52533-img0003.jpg)
![ELISA: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3H1C10-ELISA-NBP2-52533-img0008.jpg)
![Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Western-Blot-NB100-2911-img0003.jpg "Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.")
![Immunocytochemistry/Immunofluorescence: Ceramide Kinase Antibody [NB100-2911] - Ceramide Kinase antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2911-img0004.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Western-Blot-NBP2-02082-img0025.jpg "Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-02082-img0016.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0027.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0014.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0017.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0019.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0022.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0024.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0015.jpg)
![Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis using PLCG1 mouse mAb against Hela (1), A431 (2), C6 (3), NIH/3T3 (4), COS7 (5), and HCT116 (6) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Western-Blot-NBP2-52532-img0007.jpg "Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis using PLCG1 mouse mAb against Hela (1), A431 (2), C6 (3), NIH/3T3 (4), COS7 (5), and HCT116 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of HeLa cells using PLCG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Immunofluorescence-NBP2-52532-img0002.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded bladder cancer tissues using PLCG1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Immunohistochemistry-Paraffin-NBP2-52532-img0004.jpg)
![Flow Cytometry: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of Jurkat cells using PLCG1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Flow-Cytometry-NBP2-52532-img0001.jpg)
![Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Western blot analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Western-Blot-NBP2-52532-img0006.jpg)
![Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Western-Blot-NBP2-52532-img0005.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded cervical cancer tissues using PLCG1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-Immunohistochemistry-Paraffin-NBP2-52532-img0003.jpg)
![ELISA: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-1-Antibody-3C2D11-ELISA-NBP2-52532-img0009.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0005.jpg "Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.")
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM5.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0006.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis (surface staining) of human peripheral blood with anti-human CD38 (HIT2) Pacific Blue(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0012.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis using the PE conjugate of NB500-510. Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0002.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0003.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) FITC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0004.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0007.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PerCP.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0008.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0009.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0010.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Pacific Orange(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0011.jpg)
![Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NB600-920-img0004.jpg "Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.")