| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-40256SS | |||
| Azide and BSA Free | NBP2-80583 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75478SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71765 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70524 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77251-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33277-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84142-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86010-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76675-0.025mg | |||
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/antibody/ATF6-Antibody-70B1413.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 in mouse liver tissue using 3 ug/ml of ATF6 antibody and 0.25 ug/ml of GAPDH antibody. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Western-Blot-NBP1-40256-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody (70B1413.1) [NBP1-40256] - Untreated HeLa cells were fixed in -20C methanol for 10 min, air dried and rehydrated in PBS at room temperature for 5 minutes. Cells were incubated with anti-ATF6 (1:20) for one hour at room temperature. ATF6 reactivity (green) was detected with anti-mouse Dylight-488 secondary antibody. Nuclei were counterstained with DAPI (blue). Note the ER localization of ATF6.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Immunocytochemistry-Immunofluorescence-NBP1-40256-img0014.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 antibody on Human placenta. Fixed paraffin-embedded sections. Antibody dilution 1:50. Incubated overnight in 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Immunohistochemistry-Paraffin-NBP1-40256-img0017.jpg)
![Flow (Intracellular): ATF6 Antibody (70B1413.1) [NBP1-40256] - An intracellular stain was performed on HeLa cells with ATF6 Antibody (70B1413.1) NBP1-40256F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Flow-Intracellular-NBP1-40256-img0016.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody and visualized with PicoTect Western Blot Chemiluminescence Substrate (10087K). Film was exposed for 1 min. The top arrow corresponds to the 90 kDa form of ATF6 described as full-length in the literature. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Western-Blot-NBP1-40256-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Human placenta, followed by biotinylated horse anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Dilution 10ug/ml](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Immunohistochemistry-Paraffin-NBP1-40256-img0001.jpg)
![Flow Cytometry: ATF6 Antibody (70B1413.1) [NBP1-40256] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using ATF6 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Flow-Cytometry-NBP1-40256-img0009.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lysate of DU145 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-imgenex-img-273-western-blot-672023151028..jpg)
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lysate of PC-3 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-imgenex-img-273-western-blot-672023151236..jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - The ISR is active in the retinas of rd16 mice.Western blots & corresponding graphs of ISR markers in the retinas of rd16 mice at P15 (a) & P20 (b). At P15, p-eIF2 alpha , ATF4, CHOP, & TRB3 are significantly elevated. At P20, levels of p-eIF2 alpha , ATF4, CHOP, & GADD34 are increased. Markers of the UPR are elevated in rd16 retinas. BiP & cleaved (~50 kD) ATF6 are upregulated in the retinas of rd16 mice at P15 (c) & P20 (d). Relative density measurements correspond to the intensities of the immunoblotting bands or lanes normalized to an internal control. Data are shown as mean ± SEM. a.u. arbitrary units. P15 n = 3. P20 n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29706649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202415171347.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Expression of ER stress-associated transcription factors in NASH, NAFL & normal liver tissues beta -Actin was used as an internal control. Horizontal lines represent means of densitometry signals from the western blot analyses for all tissue groups. #, significant differences in signals between NASH & normal liver tissues (P < 0.05). Data for NAFL tissues were not used for statistical comparisons because of limited sample number (n = 2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28968997), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202415394523.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Restoration of Plk3 induction rescues ATF2 phosphorylation & ER stress resolution in Lrh-1LKO mice, & loss or gain of ATF2 transcriptional activity also alters ER stress resolution capacity.(A) Primary hepatocytes prepared from Lrh-1f/fand Lrh-1LKO mice & transduced with Ad-Plk3 or Ad-control at a MOI of 100. Cells treated 36 hr later with vehicle or tunicamycin (TM) (0.01 µg/ml) & doxycycline (1 µg/ml) to induce Plk3 or LacZ control. Nuclear protein was obtained at timepoints indicated & immunoblotted for UPR transcription factors, with TBP as a loading control. Results representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/01694), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202415293316.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - The ISR is active in the retinas of rd16 mice.Western blots & corresponding graphs of ISR markers in the retinas of rd16 mice at P15 (a) & P20 (b). At P15, p-eIF2 alpha , ATF4, CHOP, & TRB3 are significantly elevated. At P20, levels of p-eIF2 alpha , ATF4, CHOP, & GADD34 are increased. Markers of the UPR are elevated in rd16 retinas. BiP & cleaved (~50 kD) ATF6 are upregulated in the retinas of rd16 mice at P15 (c) & P20 (d). Relative density measurements correspond to the intensities of the immunoblotting bands or lanes normalized to an internal control. Data are shown as mean ± SEM. a.u. arbitrary units. P15 n = 3. P20 n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29706649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-31020241530425.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - APPswe induction of OB-senescence via ER stress.a Heat map of differentially expressed ER stress or anti-stress related genes identified by RNA-seq in control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 Td+ OB-progenitors (detail analysis was described in Methods). b RT-PCR analysis of ER stress-related genes Grp78, Atf6, Hsp90b1, Eif2ak3, Ern1, Hsp90aa1, & Hspa2 & anti-stress related gene Sirt3 gene expression in purified Td+ BMSCs from 6-MO control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 mice, *p < 0.05, **p < 0.01, ***p < 0.001, mean ± SD, n = 3, Mann–Whitney U test. c Western blot analysis of indicated protein expression in BMSCs from mice with indicated genotypes (at 6-MO). GAPDH was used as a loading control. d Quantification of data in c, *p < 0.05, **p < 0.01. mean ± SD, n = 4, Student’s t test. e Western blot analysis of indicated protein expression in BMSCs from 6-MO control & TgAPPsweOCN with or without 0.25 mM 4-PBA (4-Phenylbutyric acid) treatment. f Quantification analyses of the data in e, *p < 0.05, n = 3. g SA-beta -gal staining of 6-MO control & TgAPPsweOCN BMSCs with vehicle (Veh)(PBS) & 4-PBA treatment, respectively, scale bar, 20 µm. h Quantification of SA-beta -gal+ cell densities in g (mean ± SD; n = 5, **p < 0.01, ***p < 0.001). Two-way analysis of variance test was used in f & h. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34824365), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416154625.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Disturbance of ER homeostasis in ADTKD-UMOD.(A) ADTKD-UMOD is characterized by maturation & trafficking defect of mutant UMOD & intracellular accumulation of UMOD in TAL cells. UMOD immunolocalization revealed a diffuse cytoplasmic staining with enforcement of the luminal membrane in TAL cells of a wild-type mouse. In contrast, TAL cells of an UmodC93F mutant mouse displayed a strong paranuclear immunopositivity for UMOD. Wild-type: Umodwt mouse; UmodC93F: homozygous UmodC93F mutant mouse. Age of mice analysed: four months. Chromogen: DAB, nuclear staining: haemalum. (B) Heat map of relative expression values (z scores) showed differential abundance of several proteins localized in the ER. (C) In the outer medulla of Umod mutant mice of both mouse lines, a strong accumulation of immature UMOD was present. (D) Protein abundances of BiP, phospho-eIF2 alpha , eIF2 alpha , ATF4, both full-length (§) & cleaved activated (#) ATF6, & CHOP were increased in Umod mutant mice compared to wild-type mice. Signal intensities were corrected for GAPDH signal intensities of the same PVDF-membrane, which was stripped several times to facilitate the detection of multiple proteins. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. Data are shown as means ± SD. One-way ANOVA with Newman-Keuls’s post hoc test: p vs. wild-type, *p < 0.05; **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep42970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416171439.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - The effect of Ezetimibe on unfolded protein response (UPR) gene expression in THP-1 cells exposed to ischemia-reperfusion (IR). (a) The mRNA expression of activating transcription factor 6 (ATF6) & CCAAT-enhancer-binding protein homologous protein (CHOP). (b) Representative Western blot analyses for the indicated proteins. (c,d) The average quantification of ATF6 & CHOP obtained by the densitometric analysis of three independent experiments. mRNA was analyzed by quantitative real-time PCR; normalized gene expression levels are given as the ratio between the mean value for the target gene & that for beta -actin in each sample. Data are expressed as mean ± SD. * p < 0.01 vs. control (up-regulation); ** p < 0.01 vs. IR; §p < 0.01 vs. control (down-regulation). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32340270), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416171424.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - APPswe induction of OB-senescence via ER stress.a Heat map of differentially expressed ER stress or anti-stress related genes identified by RNA-seq in control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 Td+ OB-progenitors (detail analysis was described in Methods). b RT-PCR analysis of ER stress-related genes Grp78, Atf6, Hsp90b1, Eif2ak3, Ern1, Hsp90aa1, & Hspa2 & anti-stress related gene Sirt3 gene expression in purified Td+ BMSCs from 6-MO control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 mice, *p < 0.05, **p < 0.01, ***p < 0.001, mean ± SD, n = 3, Mann–Whitney U test. c Western blot analysis of indicated protein expression in BMSCs from mice with indicated genotypes (at 6-MO). GAPDH was used as a loading control. d Quantification of data in c, *p < 0.05, **p < 0.01. mean ± SD, n = 4, Student’s t test. e Western blot analysis of indicated protein expression in BMSCs from 6-MO control & TgAPPsweOCN with or without 0.25 mM 4-PBA (4-Phenylbutyric acid) treatment. f Quantification analyses of the data in e, *p < 0.05, n = 3. g SA-beta -gal staining of 6-MO control & TgAPPsweOCN BMSCs with vehicle (Veh)(PBS) & 4-PBA treatment, respectively, scale bar, 20 µm. h Quantification of SA-beta -gal+ cell densities in g (mean ± SD; n = 5, **p < 0.01, ***p < 0.001). Two-way analysis of variance test was used in f & h. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34824365), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416154629.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Effect of rapamycin on markers of the UPR pathways(A) Cells were incubated in medium alone (0.05% FBS), with 3 nM IGF-1 or with both 3 nM IGF-1 & 10 nM rapamycin (IGF-1 + rapamycin). Total protein extracts prepared from cells incubated for 24 h in those conditions were subjected to Western Blot analysis. Protein expression levels were assessed for phosphorylated or total forms of PERK, eIF2 alpha & for CHOP, BiP, XBP1-s & XBP1-u proteins. Efficiency of rapamycin to inhibit mTORC1 pathway was also checked by immunoblot with phosphorylated & total forms of p70S6K1 & 4E-BP1. alpha -tubulin was used as internal control. (B & C) Cells were incubated with 10nM rapamycin for 1 h (B) or 24 h (C). Immunoblots for phosphorylated & total forms of PERK, eIF2 alpha , p70S6K1 & 4E-BP1 or for ATF4, CHOP, BiP, XBP1-s & XBP1-u protein expression were performed. alpha -tubulin was used as internal control. Blots of P-p70S6K1, p70S6K1, P-PERK, PERK, & alpha -tubulin of Figure 4B & blots of Figure 4C have been performed on the same electrophoresis gel, but cut & reconstituted. (D) Cells were incubated in medium alone (Ctrl) or with 10 nM rapamycin for 24 h. Nuclear localization of ATF6 was assessed using immunofluorescence with ATF6-antibody (red) & Hoechst dye. Magnification ×1000. (E) Bar graphs were obtained by quantification of ATF6 nuclear staining. Results are representative of at least 3 experiments. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416161822.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Activation of the UPR during hypoxia or glucose depletionSTC-1 & GluTag cells were subjected to hypoxia (1%) or cultivated with decreasing concentration of glucose i.e. 25, 5 or 1 mM, for 24 h. (A) & (C) Protein expression level of phosphorylated & total forms of PERK, eIF2 alpha & ATF4, CHOP, BiP & C-Caspase 3 protein expression was examined using immunoblots, during hypoxia (A) & glucose depletion (C). alpha -tubulin was used as internal control. Blots of Figure 2A have been performed on the same electrophoresis gel, but cut & reconstituted. (B) & (D) XBP1 mRNA splicing was analyzed by RT-PCR after Pst1 digestion: XBP1-u, unspliced; XBP1-h, hybrid; XBP1-s, spliced variant of XBP1; *, XBP1-u mRNA fragments after Pst1 digestion. (E) ATF6 nuclear localization was assessed in STC-1 cells using immunofluorescence with anti-ATF6 antibody & hoechst dye. Magnification x1000. Results are presentative of at least 3 independent experiments (A–E). (F) Bar graphs were obtained by quantification of ATF6 nuclear staining (*P < 0.05 versus control). Results are presentative of 3 independent experiments (A–E) or the mean ± S.E.M. of an experimental n = 3 (F). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-31020241616379.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - UPR status in STC-1 & GluTag cell lines & effect of UPR inducers on markers of the UPR pathwaysSTC-1 & GluTag cells were incubated in medium (Ctrl) or ER stress-inducing agents thapsigargin (Tg, 300 nM), tunicamycin (Tn, 0.05 μg/mL) & brefeldin A (Bref A, 3 μM) for 4 h, 16 h & 8 h respectively. (A) Protein expression level of phosphorylated or total forms of PERK, eIF2 alpha & CHOP, BiP & cleaved-caspase 3 (C-Caspase 3) protein expression was examined using Western Blot analysis. alpha -tubulin was used as internal control (B) Densitometric quantification of P-PERK/PERK, P-eIF2 alpha /eIF2 alpha & BiP/ alpha -tubulin ratios analysis in STC-1 or GluTag cell lines (*P < 0.05 versus control). (C–D) The effect of Tg, Tn & Bref A on ATF6 nuclear localization was assessed by immunofluorescence in STC-1 cells (C) & GluTag cells (D) using anti-ATF6 antibody & Hoechst dye. Magnification ×1000. (E) Bar graphs obtained by quantification of ATF6 nuclear staining (*P < 0.05 versus control). (F) XBP1 mRNA splicing was analyzed by RT-PCR after Pst1 digestion: XBP1-h, hybrid, XBP1-u, unspliced; XBP1-s, spliced variant of XBP1; *, XBP1-u mRNA fragments after Pst1 digestion. Results are presentative of 3 independent experiments (A, C, D, F) or the mean ± S.E.M. of an experimental n = 3 (B, E). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416173539.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Tm‐induced UPR increases expression of RANKL in cultured osteoblastic & osteocytic cells. (A) Western blotting of cell lysates obtained from neonatal calvaria‐derived osteoblastic cells (Calvaria Ob) treated with 2.2 µg/mL Tm for the indicated times. (B‐D) Gene expression as determined by qRT‐PCR in (B) calvaria‐derived osteoblastic cells (n = 3/group) (C) Osteoblastic UAMS‐32 cells (UAMS‐32 Ob) (n = 4/group) or (D) osteocytic MLO‐Y4 cells (MLO‐Y4 Ot) (n = 4/group),maintained in presence of vehicle (, 0.1% DMSO) or 2.2 µg/ml Tm () for 4 hours. (E) Western blot of RANKL protein in cell lysates obtained from calvaria‐derived osteoblastic cells as described in A in a separate study. Data shown are the mean & SD with individual data points. *P < .05 vs vehicle by Student's t‐test Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32259048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-310202416154623.jpg)
![Immunocytochemistry/Immunofluorescence: Mouse Monoclonal ATF6 Antibody (70B1413.1) [IMGENEX: IMG-273] [NBP1-40256] - Mice hepatocytes were stained for ATF6. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-imgenex-img-273-immunocytochemistry-immunofluorescence-25112024103118..png)
![Western Blot: Mouse Monoclonal ATF6 Antibody (70B1413.1) [IMGENEX: IMG-273] [NBP1-40256] - Western blot of the mouse liver homogenates from control (1) and alcohol-fed mice (2). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-imgenex-img-273-western-blot-25112024103253..png)
![Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.")
![<b>Biological Strategies Validation. </b>Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/ATF6-Antibody-Simple-Western-NBP1-75478-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody - BSA Free [NBP1-75478] - AICAR inhibits ER stress responses. Western analysis of cell lysates of nuclear extracts. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27562249/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0002.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0009.jpg "Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunocytochemistry-Immunofluorescence-NBP2-01787-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: GADD34 Antibody (OTI3D12) [NBP2-01787] - SAHA/JQ1 treatment promotes UPR target oscillation. To demonstrate that YAP1 suppresses UPR in vivo, tumor sections were stained from allografts expressing control (scr) and Rela shRNA as well as KP, KPY, and SAHA/JQ1-treated KP tumors for the UPR target Gadd34, which was found to be upregulated in Yap1/NF-kappaB-deficient tumors. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1142-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-NBP2-01787-img0012.jpg)
![Flow Cytometry: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Flow-Cytometry-NBP2-01787-img0010.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0011.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0005.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0006.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0007.jpg)
![Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Western-Blot-NBP2-01437-img0009.jpg "Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0015.jpg)
![Flow Cytometry: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of Jurkat cells, using anti-CYP2A6 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Flow-Cytometry-NBP2-01437-img0016.jpg)
![Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2A6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2A6.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Western-Blot-NBP2-01437-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human bladder tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human endometrium tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0013.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2A6-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-01437-img0014.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0005.jpg "Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.")
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM5.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0006.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis (surface staining) of human peripheral blood with anti-human CD38 (HIT2) Pacific Blue(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0012.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis using the PE conjugate of NB500-510. Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0002.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0003.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) FITC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0004.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0007.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PerCP.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0008.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0009.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0010.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Pacific Orange(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0011.jpg)
![Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP2-15291-img0016.jpg "Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15291-img0005.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Mouse liver. ADK stained by Adenosine kinase antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunohistochemistry-Paraffin-NBP2-15291-img0014.jpg)
![Western Blot: ADK Antibody [NBP2-15291] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADK antibody (NBP2-15291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-9102024202191.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-9102024205663.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-91020242024612.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ADK Antibody [NBP2-15291] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-161020241940573.jpg)
![Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GADD34-Antibody-Western-Blot-NB100-778-img0004.jpg "Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0007.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunohistochemistry-Paraffin-NB100-778-img0003.jpg)
![Flow Cytometry: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-Flow-Cytometry-NB100-778-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0006.jpg)
![Western Blot: dUTPase Antibody [NBP2-33277] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/dUTPase-Antibody-Western-Blot-NBP2-33277-img0005.jpg "Western Blot: dUTPase Antibody [NBP2-33277] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: dUTPase Antibody [NBP2-33277] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33277-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining in human lymph node and skeletal muscle tissues using anti-DUT antibody. Corresponding DUT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0009.jpg)
![Immunohistochemistry: dUTPase Antibody [NBP2-33277] - Staining of human tonsil shows strong cytoplasmic and nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-NBP2-33277-img0002.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of liver.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0003.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of liver cancer.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0004.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0007.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0008.jpg)
![Western Blot: DAK Antibody [NBP1-84142] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/DAK-Antibody-Western-Blot-NBP1-84142-img0006.jpg "Western Blot: DAK Antibody [NBP1-84142] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: DAK Antibody [NBP1-84142] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/DAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84142-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD38 Antibody [NBP1-86010] - Staining of human cell line A549 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86010-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD38 Antibody [NBP1-86010] - Staining of human cell line A549 shows localization to plasma membrane. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining in human tonsil and kidney tissues using NBP1-86010 antibody. Corresponding CD38 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0021.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0017.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human prostate shows strong membranous/cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0018.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human rectum shows strong membranous/cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0019.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human tonsil shows strong membranous/cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.Green: ATF6 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-271120241918610.gif "Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.Green: ATF6 Antibody Blue: DAPI staining")
![Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-76675] - Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-271120242013111.gif)
![Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-76675] - Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420432366.gif)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420272431.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 ug/ml.Green: ATF6 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420141974.gif)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420115534.jpg)
![Western Blot: ATF6 Antibody [NBP1-76675] - Analysis in EL4 cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-76675-img0005.jpg)