| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-32731SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70204 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87455-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44441 | |||
| BSA Free | NBP2-14787-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89915-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47998-0.1mg | |||
| IHC-Prediluted | NBP2-48229 | |||
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-32731-img0022.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - TRP53-regulated EndMT modulates the M1 and M2 populations of increased TAMs after radiotherapy. Immunofluorescence detection of F4/80, CD31, and Arg1, in KP tumours from WT and EC-p53KO mice, with or without irradiation (23 days after irradiation). Image collected and cropped by Citeab from the following publication (Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization. Nat Commun (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-NBP1-32731-img0033.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Simple Western lane view shows a specific band for ARG1 in human and mouse Liver lysate using ARG1 antibody (NBP1-32731) at 25 ug/ml. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-32731-img0029.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Effects of berberine on the M2 population of macrophages. Representative dot plot of M2 subpopulations in control group and the rats injected with ISO 3 and 7 days later. M2 cells were labelled with F4/80 and Arg1. Image collected and cropped by Citeab from the following publication (Protective role of berberine in isoprenaline-induced cardiac fibrosis in rats. BMC Cardiovasc Disord (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Flow-Cytometry-NBP1-32731-img0032.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Arginase 1 antibody detects Arginase 1 protein. Various whole cell extracts (30ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-32731-img0014.jpg)
![Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Analysis was performed using Arginase 1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent. Arginase 1 protein from HepG2 whole cell extracts using 5 ug of Arginase 1 antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunoprecipitation-NBP1-32731-img0015.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-32731-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 cells with Arginase 1/ARG1/liver Arginase Antibody NBP1-32731AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32731-img0034.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-32731-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Paraformaldehyde-fixed HeLa.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32731-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ARG1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32731-img0027.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Cal27 xenograft, using arginase I antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-32731-img0010.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Tissue section of the mouse liver using 1:200 dilution of ARG1 antibody (NBP1-32731). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-32731-img0026.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Hepatocellular carcinoma stained with Arginase1 1:100, pH9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-32731-img0028.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 with NBP1-32731 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Flow-Cytometry-NBP1-32731-img0025.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on RH30 cells with Arginase 1 Antibody NBP1-32731 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Flow-Cytometry-NBP1-32731-img0030.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Western blot analysis and gel protein densitometry were used for quantification of MARCH1 (NBP1-59758) and Arginase 1 (NBP1-32731) proteins on transduced macrophage D: The gel image indicates the blot results 48 and 72 hr after transduction, compared with the non-transduced cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35656075/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-32731_rabbit-polyclonal-arginase-1-arg1-liver-arginase-antibody-862023835350.jpg)
![Immunocytochemistry/ Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody - BSA Free [NBP1-32731] - Validation of the LCM/MS data from cellular protrusions using microscopy. Immunofluorescence (IF) of proteins identified by LCM/MS (A–D) “expected” or (E–H) “not expected” to be within protrusions based on their known functions/localizations. (A) Cd47 & (B) Anxa2 are found in both hCAD/dCAD protrusions. (C) Tenm2 is found within hCAD protrusions & (D) Cobl in GCs. Interestingly, (E) Grk5 was observed in hCAD protrusions; (F) Hist1h3b protein in hCAD protrusions & GCs (G) Hspa1b in dCAD protrusions & TNTs & (H) Arg1 in dCAD protrusions, GCs, & TNTs. White arrows/arrowheads show punctates within dCAD/hCAD protrusions & GCs or within TNTs, respectively. Scale bars = 10 μm. IF of all 8 proteins corroborate the LCM/MS protein identification for each subtypes of protrusions. (I) 1198 unique proteins were identified by LCM/MS to be in protrusions & 169 were found to be exclusive to protrusions. 904 of the 1198 proteins found in protrusions & 87 out of 169 exclusive proteins had images in the HPA Subcell database. IF images for the proteins identified by LCM/MS in protrusions, present in the HPA Subcell database, were observed & catalogued as being observed or not in protrusions (Figure S2). On average 4.7% of proteins are found in protrusions in the HPA database, 13.6% are found in our LCM isolated protrusions & 23% were found in the “exclusive” LCM protrusions. The fold enrichment increases by 2.9% or 4.8% when we looked at proteins identified by LCM/MS in protrusions or exclusive to protrusions, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32731_rabbit-polyclonal-arginase-1-arg1-liver-arginase-antibody-310202416165649.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NB100-59740] - (0.003ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-59740_goat-polyclonal-arginase-1-arg1-liver-arginase-antibody-1652023918335.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NB100-59740] - (0.003ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western blot results. (A) Representative Western blot and quantitative analysis of protein levels of Iba‐1, iNOS, ARG1, IL‐1 beta , and TNF‐ alpha in PFC tissues, n = 6. (B) Relative level of Iba‐1 protein in PFC (fold change relative to beta ‐actin protein level). (C) The relative level of iNOS protein in PFC (fold change relative to beta ‐actin protein level). (D) The relative level of ARG1 protein in PFC (fold change relative to beta ‐actin protein level). (E) The relative level of IL‐1 beta protein in PFC (fold change relative to beta ‐actin protein level). (F) The relative level of TNF‐ alpha protein in PFC (fold change relative to beta ‐actin protein level). *p < 0.05 versus Con group. ARG1, arginase‐1; IL‐1 beta , interleukin‐1 beta ; iNOS, inducible nitric oxide synthase; PFC, prefrontal cortex; TNF‐ alpha , tumor necrosis factor‐ alpha [Color figure can be viewed at wileyonlinelibrary.com] Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37786559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-59740_goat-polyclonal-arginase-1-arg1-liver-arginase-antibody-western-blot-132202618165113.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0008.jpg "Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining in human liver and kidney tissues . Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Analysis using Anti-ARG1 antibody NBP1-87490 (A) shows similar pattern to independent antibody NBP1-87455 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87490-img0020.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in a subset of hematopoietic cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0015.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human hepatocellular carcinoma shows moderate cytoplasmic and nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0016.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0017.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0018.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:50 dilution on Liver and HepG2 lysate(s).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0009.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0009.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Immunohistochemistry-Paraffin-NBP2-03618-img0006.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-liver Arginase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Flow-Cytometry-NBP2-03618-img0005.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver Arginase.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0007.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0008.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Jurkat cell lysate, concentration 5.0ug/ml.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-54621-img0002.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Jurkat cell lysate, concentration 5.0ug/ml.")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-54621-img0003.jpg)
![Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Genetic deletion of IL-6 results in less fibrosis and increased remodeling at a chronic time point. A) Anti-Arginase 1/ARG1/liver Arginase immunohistochemistry staining results (n = 4-5; ∗P = .0210) and representative slides. The number of Arg1+ cells, a marker for prohealing monocytes and macrophages, was less in the IL-6-/- model. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647566/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-54621_rabbit-polyclonal-arginase-1-arg1-liver-arginase-antibody-20920231412575.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Analysis using Anti-ARG1 antibody NBP1-87455 (A) shows similar pattern to independent antibody NBP1-87490 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87455-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Analysis using Anti-ARG1 antibody NBP1-87455 (A) shows similar pattern to independent antibody NBP1-87490 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining in human liver and pancreas tissues using anti-ARG1 antibody. Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0009.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87455-img0005.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0007.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0008.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Analysis in human liver and skeletal muscle tissues using NBP2-14787 antibody. Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-14787_mouse-monoclonal-arginase-1-arg1-liver-arginase-antibody-cl0186-immunohistochemistry-paraffin-194202491234..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Analysis in human liver and skeletal muscle tissues using NBP2-14787 antibody. Corresponding ARG1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Simple Western: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Simple Western lane view shows a specific band for ARG1 in human and mouse Liver lysate using ARG1 antibody (NBP2-14787) at 25 ug/ml. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-CL0186-Simple-Western-NBP2-14787-img0010.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Lane 1: Marker [kDa], Lane 2: Human liver tissue lysate](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-CL0186-Western-Blot-NBP2-14787-img0009.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Staining of human prostate shows no immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-CL0186-Immunohistochemistry-Paraffin-NBP2-14787-img0005.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-CL0186-Immunohistochemistry-Paraffin-NBP2-14787-img0004.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-CL0186-Simple-Western-NBP2-14787-img0007.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:20 dilution on Liver lysate(s).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-CL0186-Simple-Western-NBP2-14787-img0008.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-14787_mouse-monoclonal-arginase-1-arg1-liver-arginase-antibody-cl0186-immunohistochemistry-paraffin-194202491338..jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14787_mouse-monoclonal-arginase-1-arg1-liver-arginase-antibody-cl0186-immunohistochemistry-paraffin-194202491429..jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14787_mouse-monoclonal-arginase-1-arg1-liver-arginase-antibody-cl0186-immunohistochemistry-paraffin-194202491521..jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14787_mouse-monoclonal-arginase-1-arg1-liver-arginase-antibody-cl0186-immunohistochemistry-paraffin-194202491613..jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A")
![Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70204-img0005.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-liver Arginase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70204-img0004.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0001.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0002.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Western-Blot-NBP2-89915-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0002.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human liver with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89915 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-23520252135123.png)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Analysis of Human ARG1 expression on HepG2 cells. The cells were treated, stained with NBP2-89915, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-235202511545610.png)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Western Blot Analysis of human liver tissue lysate using Arginase 1/ARG1/liver Arginase antibody (ARG1/1125).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Western-Blot-NBP2-44965-img0004.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Western Blot Analysis of human liver tissue lysate using Arginase 1/ARG1/liver Arginase antibody (ARG1/1125).")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Human Hepatocellular Carcinoma stained with ARG1 Monoclonal Antibody (ARG1/1125).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Immunohistochemistry-Paraffin-NBP2-44965-img0001.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis A) Recombinant ARG1 Protein Fragment (B) human Liver Lysate using ARG1 Monoclonal Antibody (ARG1/1125).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Western-Blot-NBP2-44965-img0002.jpg)
![Protein Array: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Protein-Array-NBP2-44965-img0003.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Analysis of Pig (A), Mouse (B) and Rat (C) Liver lysates (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-36936-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Analysis of Pig (A), Mouse (B) and Rat (C) Liver lysates (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Staining of paraffin embedded Human Liver. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-36936-img0005.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-36936-img0002.jpg)