| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30027SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71765 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32026-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31974-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87423-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89061-25ul | |||
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30027-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-30027-img0015.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0019.jpg "Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunocytochemistry-Immunofluorescence-H00002629-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunohistochemistry-Paraffin-H00002629-M01-img0014.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - GBA monoclonal antibody (M01), clone 2E2 Analysis of GBA expression in MCF-7.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0016.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0017.jpg)
![ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-ELISA-H00002629-M01-img0018.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0011.jpg "Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.")
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0008.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0002.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0009.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0003.jpg)
![Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Sandwich-ELISA-H00055743-M01-img0010.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0009.jpg "Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunocytochemistry-Immunofluorescence-NBP2-01787-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: GADD34 Antibody (OTI3D12) [NBP2-01787] - SAHA/JQ1 treatment promotes UPR target oscillation. To demonstrate that YAP1 suppresses UPR in vivo, tumor sections were stained from allografts expressing control (scr) and Rela shRNA as well as KP, KPY, and SAHA/JQ1-treated KP tumors for the UPR target Gadd34, which was found to be upregulated in Yap1/NF-kappaB-deficient tumors. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1142-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-NBP2-01787-img0012.jpg)
![Flow Cytometry: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Flow-Cytometry-NBP2-01787-img0010.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0011.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0005.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0006.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - All lanes: Western blot analysis of GBA with anti-GBA antibody [JM10-76 at 1:1,000 dilution.Lane 1: Wild-type HEK293T whole cell lysate (20 ug).Lane 2: GBA knockout HEK293T whole cell lysate (20 ug). NBP2-66871was shown to specifically react with GBA in wild-type HEK293T cells. No band was observed when GBA knockout sample was tested. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0011.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0015.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Western blot analysis of Glucosylceramidase/GBA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0009.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0001.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human liver tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0003.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat liver tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0014.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Immunohistochemistry-Paraffin-NBP1-32271-img0008.jpg "Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Western-Blot-NBP1-32271-img0013.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-91020242013402.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-1610202419414552.jpg)
![Glucosylceramidase/GBA antibody [C1C3] detects Glucosylceramidase/GBA protein by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glucosylceramidase/GBA stained by Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-immunocytochemistry-immunofluorescence-141120251833710.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-western-blot-1411202518431318.jpg)
![Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GADD34-Antibody-Western-Blot-NB100-778-img0004.jpg "Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0007.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunohistochemistry-Paraffin-NB100-778-img0003.jpg)
![Flow Cytometry: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-Flow-Cytometry-NB100-778-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0006.jpg)
![Western Blot: PLC-beta 3 Antibody [NBP2-32026] - Analysis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar pattern to independent antibody NBP2-31974 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-32026-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PLC-beta 3 Antibody [NBP2-32026] - Analysis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar pattern to independent antibody NBP2-31974 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PLC-beta 3 Antibody [NBP2-32026] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32026-img0007.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human duodenum.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0012.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody [NBP2-32026] - Staining of human small intestine shows moderate cytoplasmic and luminal membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-NBP2-32026-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human cerebral cortex, duodenum, lymph node and testis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar protein distribution across tissues to independent antibody NBP2-31974 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0008.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0011.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human testis.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0013.jpg)
![Western Blot: FHIT Antibody [NBP1-89062] - Analysis in control (vector only transfected HEK293T lysate) and fHIT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FHIT-Antibody-Western-Blot-NBP1-89062-img0005.jpg "Western Blot: FHIT Antibody [NBP1-89062] - Analysis in control (vector only transfected HEK293T lysate) and fHIT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89062] - Staining of human colon.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89062-img0009.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89062] - Staining of human kidney shows strong cytoplasmic positivity in tubule cells.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89062-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89062] - Staining of human colon, kidney, lymph node and testis using Anti-FHIT antibody NBP1-89062 (A) shows similar protein distribution across tissues to independent antibody NBP1-89061 (B).](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89062-img0006.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89062] - Staining of human lymph node.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89062-img0007.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89062] - Staining of human kidney.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89062-img0008.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89062] - Staining of human testis.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89062-img0010.jpg)
![Western Blot: PLC-beta 3 Antibody [NBP2-31974] - Analysis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar pattern to independent antibody NBP2-32026 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-31974-img0015.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PLC-beta 3 Antibody [NBP2-31974] - Analysis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar pattern to independent antibody NBP2-32026 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PLC-beta 3 Antibody [NBP2-31974] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31974-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human testis.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0018.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody [NBP2-31974] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-NBP2-31974-img0008.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0011.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining in human small intestine and cerebral cortex tissues using anti-PLCB3 antibody. Corresponding PLCB3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human cerebral cortex, duodenum, lymph node and testis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar protein distribution across tissues to independent antibody NBP2-32026 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0014.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human duodenum.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0016.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0017.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Staining of human colon, kidney, liver and pancreas using Anti-MUT antibody NBP1-87423 (A) shows similar protein distribution across tissues to independent antibody NBP2-34024 (B).](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP1-87423-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Staining of human colon, kidney, liver and pancreas using Anti-MUT antibody NBP1-87423 (A) shows similar protein distribution across tissues to independent antibody NBP2-34024 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Western-Blot-NBP1-87423-img0006.jpg)
![Western Blot: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Western-Blot-NBP1-87423-img0005.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP1-87423-img0002.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Staining of human pancreas.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP1-87423-img0008.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Staining of human colon.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP1-87423-img0009.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP1-87423] - Staining of human liver.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP1-87423-img0011.jpg)
![Western Blot: FHIT Antibody [NBP1-89061] - Analysis in control (vector only transfected HEK293T lysate) and FHIT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FHIT-Antibody-Western-Blot-NBP1-89061-img0006.jpg "Western Blot: FHIT Antibody [NBP1-89061] - Analysis in control (vector only transfected HEK293T lysate) and FHIT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human testis.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0010.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human kidney shows strong cytoplasmic positivity in tubules and weak staining in glomeruli.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human colon, kidney, lymph node and testis using Anti-FHIT antibody NBP1-89061 (A) shows similar protein distribution across tissues to independent antibody NBP1-89062 (B).](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0007.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human colon.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0008.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human kidney.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0009.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human lymph node.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0011.jpg)