| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-93479SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92006-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37245-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95251-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88655-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95253-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13813-25ul | |||
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were subjected to a glycolysis stress test as described under](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were subjected to a glycolysis stress test as described under")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] antibody (1:250) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93479-img0006.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator. Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0003.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle. Samples were resolved by electrophoresis, transferred and probed by antibodies and representative immunoblots and normalization of pPDH are presented. The following comparisons were made: within a genotype, fed versus fasted; across genotypes, fed versus fed and fasted versus fasted. Bars that do not share a common symbol differ significantly (P < 0.05). Data presented as mean +/- SEM (n = 6). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0124204), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0008.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free [NB110-93479] - PDK4 expression during syncytialization of human placental trophoblasts. (A) Changes of the RPKM values for PDK family members in trophoblasts before (red column, 3 hours) & after (blue column, 48 hours) syncytialization. (B) Changes in PDK4 mRNA (black column, n = 4) & protein (white column, n = 4) abundance during syncytialization. (C) Changes in the phosphorylation of PDHE1 alpha during syncytialization n = 4. (D) Representative images showing intense staining of PDK4 (red) in the cytotrophoblast layer & weak staining of PDK4 in the syncytial layer of human chorionic villi at early gestation. The syncytial & cytotrophoblast layers were labeled with beta -hCG (green) & SPINT1 (green) respectively. Nuclei were counterstained with DAPI (blue). n = 3; *P < 0.05; **P < 0.01; ***P < 0.001 against 3 hours; n.s., not significant. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-09163-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93479_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-antibody-310202416212313.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0015.jpg "Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33527-img0002.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Rat colon. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0013.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGEHPRT antibody dilution: 1:500](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0009.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0011.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Human endometrial cancer. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0005.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Colon cancer. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0006.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Rat brain. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0007.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Mouse colon. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0012.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.HPRT stained by HPRT antibody (NBP1-33527) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33527_rabbit-polyclonal-hprt-antibody-910202420115025.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.HPRT stained by HPRT antibody (NBP1-33527) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33527_rabbit-polyclonal-hprt-antibody-910202420104479.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HPRT Antibody [NBP1-33527] - Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (10 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33527_rabbit-polyclonal-hprt-antibody-1610202419414546.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0020.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Analysis in human cell lines HeLa and HEK293 using anti-B4GALT1 antibody. Corresponding B4GALT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Western-Blot-NBP1-88654-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88654-img0007.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0023.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0022.jpg)
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0007.jpg "Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0004.jpg)
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Hela cell using anti-HPRT antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: A431Lane 3: MCF-7 Lane 4: 293Lane 5: Rat kidney Lane 6: Rat brainLane 7: Mouse testis Lane 8: Mouse colon](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0005.jpg)
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0006.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0001.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0002.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human kidney tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0003.jpg)
![Western Blot: HPRT Antibody [NBP1-92006] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-92006-img0007.jpg "Western Blot: HPRT Antibody [NBP1-92006] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-92006] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92006-img0008.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human testis shows very strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0019.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0016.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0017.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0018.jpg)
![Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Western-Blot-NBP2-37245-img0004.jpg "Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.")
![Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) [NBP2-37245] - Analysis of kidney tissues using HPRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Immunohistochemistry-NBP2-37245-img0006.jpg)
![Flow (Intracellular): HPRT Antibody (5F11A7) [NBP2-37245] - An intracellular stain was performed on HeLa cells with HPRT Antibody (5F11A7) NBP2-37245APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Flow-Intracellular-NBP2-37245-img0007.jpg)
![Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against human HPRT1 (AA: FULL(1-218)) recombinant protein. (Expected MW is 50.5 kDa)](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Western-Blot-NBP2-37245-img0002.jpg)
![Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Western-Blot-NBP2-37245-img0003.jpg)
![Flow Cytometry: HPRT Antibody (5F11A7) [NBP2-37245] - Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Flow-Cytometry-NBP2-37245-img0005.jpg)
![ELISA: HPRT Antibody (5F11A7) [NBP2-37245] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-ELISA-NBP2-37245-img0001.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Western-Blot-NBP2-33922-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - PDHA2 Antibody [NBP2-33922] - Staining of human cell line HeLa shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33922-img0005.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] -Staining of human heart muscle shows strong cytoplasm granular positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-174202494855..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Staining of human kidney shows strong cytoplasm granular positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-17420249518..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Staining of human testis shows strong cytoplasm granular positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-174202495223..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-33922] - Staining of human duodenum shows moderate cytoplasm granular positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33922_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-174202495338..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Analysis of extracts of various cell lines, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Western-Blot-NBP2-95251-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Analysis of extracts of various cell lines, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-95251-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-95251-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95251] - Immunofluorescence analysis of NIH/3T3 cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95251_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-5220251640291.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88655 (A) shows similar protein distribution across tissues to independent antibody NBP1-88654 (B).](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88655-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88655 (A) shows similar protein distribution across tissues to independent antibody NBP1-88654 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88655-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88655-img0014.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88655-img0010.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88655-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88655] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88655-img0013.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-western-blot-241202412935..jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] -Analysis of paraffin-embedded rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121118..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121229..jpg)
![Immunocytochemistry/Immunofluorescence: PRPS1 Antibody [NBP2-13813] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13813-img0003.jpg "Immunocytochemistry/Immunofluorescence: PRPS1 Antibody [NBP2-13813] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP2-13813] - Staining of human prostate shows weak nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunohistochemistry-Paraffin-NBP2-13813-img0007.jpg)
![Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP2-13813] - Staining of human endometrium shows moderate nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunohistochemistry-Paraffin-NBP2-13813-img0004.jpg)
![Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP2-13813] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunohistochemistry-Paraffin-NBP2-13813-img0005.jpg)
![Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP2-13813] - Staining of human pancreas shows low positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PRPS1-Antibody-Immunohistochemistry-Paraffin-NBP2-13813-img0006.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-288202412442923.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32787) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-298202414115418.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling Pyruvate Dehydrogenase E1-alpha subunit with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-2920241114815.jpg)