| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90264-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73362 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70074 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38542-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86121-25ul | |||
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0007.jpg "Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-32781] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32781-img0009.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781] - Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32781-img0006.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:20000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0005.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781] - HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [C1C3]. Western blot analysis was performed using Aconitase 2 antibody [C1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP1-32781-img0011.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-31435-img0005.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP1-31435] - Analysis of HaLa, using NBP1-31435 at 1:200 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31435-img0003.jpg)
![Immunohistochemistry: PRPS2 Antibody [NBP1-31435] - Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 x 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 x 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 x 1 = 1. Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-NBP1-31435-img0007.jpg)
![Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-31435] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-Paraffin-NBP1-31435-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PRPS2 Antibody [NBP1-31435] - The effects of PRPS2 knockdown confirmed by qRT-PCR (top) and Western blotting (bottom). Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Knockdown-Validated-NBP1-31435-img0006.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 decreased prostate cancer cell growth. (A) The effects of PRPS2 knockdown confirmed by qRT-PCR (top) & Western blotting (bottom). (B) In the CCK‑8 assay, cell viability was decreased in si‑PRPS2 compared with NC. (C) In the plate colony formation assay, colony formation was obviously decreased in si-PRPS2 cells transfected. ***P < 0.001. NC: negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202415345324.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 inhibits xenograft tumor growth in vivo. (A) PRPS2 shRNA effectively blocked its protein expression in PC-3 cells. Cells were stably transfected with scramble (sh-Ctrl) or PRPS2 shRNA (sh-PRPS2). PRPS2 protein levels were normalized to alpha -Tubulin. (B) Gross observation of xenograft tumor size in NOD/SCID mice. (C & D) Silencing of PRPS2 inhibited the tumor growth, including tumor volume (P < 0.001) & weight (P = 0.028, n = 6). (E) H&E‑stained paraffin‑embedded sections obtained from xenografts of PC-3 cells. (F) Top: Immunohistochemical analysis of Ki-67 in the xenografts; Bottom: The apoptosis in tumor tissues was evaluated by TUNEL assay (×200). Graphical illustrated the quantification of Ki-67 & TUNEL positive cells percentage. ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202416163792.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - PRPS2 expression in prostate cancer tissues & prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), & with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, & in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 & its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 & its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 & its total protein expression score is 1 × 1 = 1. (B) Western blotting & real-time qPCR analysis of PRPS2 protein & mRNA expression in prostate cancer cell lines & normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-3102024161890.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-90264] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90264-img0008.jpg "Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-90264] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining in human heart muscle and liver tissues using NBP1-90264 antibody. Corresponding ACO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0018.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0015.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0017.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human duodenum shows strong strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0014.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human kidney shows strong strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0016.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-310202416113522.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-310202416121946.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-31020241612192.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0012.jpg "Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse intestine. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0011.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Aconitase 2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0005.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0007.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse urinary bladder. Aconitase 2 antibody [N1N3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0008.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse ovary. Aconitase 2 antibody [N1N3] dilution: 1:500](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0010.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245] - Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [N1N3] . Western blot analysis was performed using Aconitase 2 antibody [N1N3] .EasyBlot anti-Rabbit IgG was used as a secondary reagent](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP2-15245-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody [NBP2-15245] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (NBP2-15245) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-910202420134052.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP2-15245 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-9102024205635.jpg)
![Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] (NBP2-15245) diluted at 1:1000.Red: MitoTrackerR Red CMXRos, a mitochondria tracker.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-immunocytochemistry-immunofluorescence-2611202518384020.jpg)
![Western Blot: PGM3 Antibody (1A11) [NBP2-03734] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Western-Blot-NBP2-03734-img0009.jpg "Western Blot: PGM3 Antibody (1A11) [NBP2-03734] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human prostate tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0008.jpg)
![Flow Cytometry: PGM3 Antibody (1A11) [NBP2-03734] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGM3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Flow-Cytometry-NBP2-03734-img0001.jpg)
![Western Blot: PGM3 Antibody (1A11) [NBP2-03734] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Western-Blot-NBP2-03734-img0002.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0003.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0004.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human Kidney tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0005.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human liver tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0006.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human pancreas tissue using anti-PGM3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-03734-img0007.jpg)
![Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Western-Blot-NBP2-02241-img0001.jpg "Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase 2.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02241-img0006.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02241-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] - Immunofluorescent staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02241-img0002.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02241-img0003.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02241-img0004.jpg)
![Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PGM3-Antibody-1E2-1B12-Western-Blot-H00005238-M01-img0012.jpg "Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/PGM3-Antibody-1E2-1B12-Immunohistochemistry-Paraffin-H00005238-M01-img0007.jpg)
![Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi ( Cat # H00005238-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # H00005238-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PGM3-Antibody-1E2-1B12-Western-Blot-H00005238-M01-img0009.jpg)
![Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - PGM3 monoclonal antibody (M01), clone 1E2-1B12 Analysis of PGM3 expression in HeLa.](http://images.novusbio.com/fullsize/PGM3-Antibody-1E2-1B12-Western-Blot-H00005238-M01-img0011.jpg)
![Immunoprecipitation: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1E2-1B12-Immunoprecipitation-H00005238-M01-img0008.jpg)
![Sandwich ELISA: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Detection limit for recombinant GST tagged PGM3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-1E2-1B12-Sandwich-ELISA-H00005238-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining in human parathyroid gland and liver tissues using anti-GPD2 antibody. Corresponding GPD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining in human parathyroid gland and liver tissues using anti-GPD2 antibody. Corresponding GPD2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP2-38542 (A) shows similar protein distribution across tissues to independent antibody NBP1-86121 (B).](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GPD2 Antibody [NBP2-38542] - Analysis using Anti-GPD2 antibody NBP2-38542 (A) shows similar pattern to independent antibody NBP1-86121 (B).](http://images.novusbio.com/fullsize/GPD2-Antibody-Western-Blot-NBP2-38542-img0010.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0015.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0006.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human colon.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0011.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human skin.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0012.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human liver using Anti-GPD2 antibody NBP2-38542.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0013.jpg)
![Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human parathyroid gland using Anti-GPD2 antibody NBP2-38542.](http://images.novusbio.com/fullsize/GPD2-Antibody-Immunohistochemistry-Paraffin-NBP2-38542-img0014.jpg)
![Western Blot: GPD2 Antibody [NBP1-86121] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP1-86121 (A) shows similar protein distribution across tissues to independent antibody NBP2-38542 (B).](http://images.novusbio.com/fullsize/GPD2-Antibody-Western-Blot-NBP1-86121-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GPD2 Antibody [NBP1-86121] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP1-86121 (A) shows similar protein distribution across tissues to independent antibody NBP2-38542 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: GPD2 Antibody [NBP1-86121] - Analysis using Anti-GPD2 antibody NBP1-86121 (A) shows similar pattern to independent antibody NBP2-38542 (B).](http://images.novusbio.com/fullsize/GPD2-Antibody-Western-Blot-NBP1-86121-img0011.jpg)
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0005.jpg "Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-5220251242556.jpg)
![Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunofluorescence analysis of NIH-3T3 cells using Aconitase 2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-5220251242512.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-52202511383784.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Aconitase 2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16426_rabbit-aconitase-2-mab-0p3h3-52202511361218.jpg)
![Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70074-img0001.jpg "Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0003.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0002.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0004.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0006.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0005.jpg "Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52015] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52015-img0002.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Aconitase 2 (aa541-555) in Heart lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Human (A), Mouse (B) and Rat (C) Adipose lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0004.jpg)