| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51691 | |||
| BSA Free | NBP1-51691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77106-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| BSA Free | NBP2-60735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83826-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - Carbonic Anhydrase IX mouse antibody against Hela (1) and A549 (2) cell lysates. Bands were detected at a molecular weight of approximately 50 kDa in both cell lines. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Azide-and-BSA-Free-Western-Blot-NBP2-80611-img0001.jpg "Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - Carbonic Anhydrase IX mouse antibody against Hela (1) and A549 (2) cell lysates. Bands were detected at a molecular weight of approximately 50 kDa in both cell lines. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - Mouse choroid stained with anti-Carbonic Anhydrase IX antibody. Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80611-img0003.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - Paraffin-embedded lung tissues (left) and colonic tissues (right) using Carbonic Anhydrase IX mouse antibody with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80611-img0004.jpg)
![Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - A surface stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 NBP1-51691 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80611-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CA9 (2D3) [NBP1-51691] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80611-img0002.jpg)
![Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - Azide and BSA Free [NBP2-80611] - Analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80611-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue with LXR-A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunohistochemistry-Paraffin-NBP1-77106-img0002.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue lysate at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Rat liver tissue with LXR-A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunofluorescence-NBP1-77106-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419541563.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419313242.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419553048.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419432221.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NB100-1471-img0005.jpg "Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GADD34-Antibody-Western-Blot-NB100-778-img0004.jpg "Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0007.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunohistochemistry-Paraffin-NB100-778-img0003.jpg)
![Flow Cytometry: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-Flow-Cytometry-NB100-778-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0006.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0007.jpg "Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color")
![Immunohistochemistry: CPT1B Antibody [NBP1-59576] - searcher: Dr. Pankaj Kumar Singh, UNMC, Omaha, NE Application: IHC Species+tissue/cell type:Species+tissue/cell type: Human Capan1 cells Primary antibody dilution: 1:300 Secondary antibody: Anti-rabbit Alexa Fluor 488 Secondary antibody dilution:1:200](http://images.novusbio.com/fullsize/CPT1B-Antibody-Immunohistochemistry-NBP1-59576-img0004.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0003.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0006.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Western Blot: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell lysateLane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysateLane 5: human lung carcinoma tissue lysate.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Western-Blot-NBP2-67766-img0007.jpg "Western Blot: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell lysateLane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysateLane 5: human lung carcinoma tissue lysate.")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (SD20-92) [NBP2-67766] - Staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunocytochemistry-Immunofluorescence-NBP2-67766-img0005.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded mouse colon tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0017.jpg)
![Flow Cytometry: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Flow-Cytometry-NBP2-67766-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human liver carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0008.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human prostate carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0009.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human skin tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0010.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0011.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human stomach carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0012.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0014.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human thyroid tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0015.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human small intestine tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0016.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Western-Blot-H00051703-M01-img0012.jpg "Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Immunohistochemistry-Paraffin-H00051703-M01-img0009.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody (M01), clone 5H8.Lane 1: ACSL5 transfected lysate(82.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Western-Blot-H00051703-M01-img0013.jpg)
![Immunoprecipitation: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 transfected lysate using anti-ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Immunoprecipitation-H00051703-M01-img0010.jpg)
![Sandwich ELISA: ACSL5 Antibody (5H8) [H00051703-M01] - Detection limit for recombinant GST tagged ACSL5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Sandwich-ELISA-H00051703-M01-img0011.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Representative western blotting (n = 3) for U87Empty, U87WT, and U87Mut cell protein extracts. The membranes were blotted with ACSL5 (H00051703-M01). The relative quantification of the bands is shown under the line. Blot was stripped and reprobed with anti-beta actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35574033/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00051703-m01_mouse-monoclonal-acsl5-antibody-5h8-25520231539511.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Effects of IGF1R localization over cell metabolism. (A) U87Empty, U87WT, & U87Mut cells were cultured 24 h in serum-free medium or with 50-nM IGF1 stimulation (IGF1). To test the specificity of the response, pre-incubation (1 h) with 0.5 μM OSI906 was also performed (IGF1+ OSI). (A, B) GLUT1 mRNA expression was calculated by rqPCR by the relative quantitation method. (A) Values are presented as fold change compared to control conditions (serum-free). (B) IGF1 stimuli comparison between cell lines. The results are presented as fold change due to IGF1 stimulation over basal conditions (serum-free). Values are expressed as mean ± SD of three independent experiments performed in triplicates (*p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001, ANOVA, Tukey’s post-test). (C) LDH enzyme activity was measured & normalized to DNA content (mg). The results are expressed as mean ± SD of three independent experiments (**p < 0.005, ANOVA, Tukey’s post-test). (D) Representative western blotting (n = 3) for U87Empty, U87WT, & U87Mut cell protein extracts. The membranes were blotted with anti-pPDC (line 1), anti-FAS (line 3), anti-ACSL5 (line 5), & anti-pS6K (line 7). Each blot was stripped & reprobed with anti-beta actin (lines 2, 4, & 6) or antitotal-S6K (line 8). The relative quantification of the bands is shown under each line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35574033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00051703-m01_mouse-monoclonal-acsl5-antibody-5h8-310202415482271.jpg)
![Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Western-Blot-NBP2-80831-img0001.jpg "Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80831-img0002.jpg)
![Immunohistochemistry: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80831-img0003.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Simple-Western-NBP2-80831-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80831-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: OGFOD1 Antibody [NBP1-83826] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/OGFOD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83826-img0017.jpg "Immunocytochemistry/Immunofluorescence: OGFOD1 Antibody [NBP1-83826] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: OGFOD1 Antibody [NBP1-83826] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/OGFOD1-Antibody-Immunohistochemistry-Paraffin-NBP1-83826-img0019.jpg)
![Immunohistochemistry-Paraffin: OGFOD1 Antibody [NBP1-83826] - Staining of human skeletal muscle shows moderate to strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/OGFOD1-Antibody-Immunohistochemistry-Paraffin-NBP1-83826-img0018.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)